Sıçan Eritrositlerinde Karbon Tetraklorürün(CCl4) ve Kuersetinin Bazı Metabolik Enzim Aktiviteleri Üzerine İn Vivo Etkisinin İncelenmesi

Öz

Çalışmada; Sıçan eritrositlerinde karbon tetraklorür (CCl4) ve Kuersetinin(Ku)'in, Glukoz 6-fosfat dehidrogenaz (G6PD), 6-fosfoglukonat dehidrogenaz (6PGD), glutatyon redüktaz (GR) ve glutatyon S-transferaz (GST) gibi önemli metabolik enzim aktiviteleri üzerine in vivoetkilerinin incelenmesi hedeflendi.Deneysel aşamadasıçanlar 4 gruba ayrıldı. 1.Grup(Kontrol): Bu gruptaki ratlara vücut ağırlıklarına göre belirlenen dozda(1 mL/kg)saf zeytin yağı, 2.Grup(CCl4: 1.0 mL/kg (i.p.)(1:1), 3.Grup(Ku: 25 mg/kg (i.p),4.Grup(CCl4(1.0 ml/kg (i.p.)+ Ku (25 mg/kg (i.p.) enjekte edildi.Çalışma 3günsürdürüldü.Sonuçlar değerlendirildiğinde; G6PD (p˂0,01), 6PGD (p˂0,01) GR (p˂0,001) ve GST (p˃0,05) enzim aktiviteleri kontrol grubuna oranla CCl4grubunda azalmıştır. CCl4+Ku uygulanan gruplarda ise enzim aktivitelerinin CCl4grubuna oranla yüksekolduğu belirlenmiştir.Ku uygulanması, enzim aktivite değerinin yükselmesine neden olmuştur. BudaCCI4’ün neden olduğu inhibisyonun ortadan kalktığının göstergesi olarak kabul edilebilir. Sonuç olarak, CCl4uygulamasına bağlı olarak ortaya çıkan oksidatif hasarı önlemede Ku'nın etkili olabileceği düşünülmektedir.

Anahtar Kelimeler

• CCl4
• ,Ku
• ,G6PD
• ,6PGD
• ,GR

Investigation of In Vivo Effects of Carbon Tetrachloride (CCl4) and Quercetin on Some Metabolic Enzyme Activities in Rat Erythrocyte

Abstract

In the study; the purpose was to investigate the in vivoimpact of carbon tetrachloride (CCl4) and quercetin (Qu) on activities of important metabolic enzymes such as Glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR) and glutathione S-transferase (GST) in rat erythrocytes. At the experimental stage, rats were divided into 4 groups. 1.Group (Control): Pure olive oil at a dose determined according to their body weight (1mL/kg) was given to the rats in this group, 2.Group (CCl4: 1.0 mL/kg (ip)(1:1), 3.Group (Ku: 25) mg/kg (ip), 4.Group (CCl4(1.0 ml/kg (ip)+ Ku (25 mg/kg (ip) was injected. The study was continued for 3 days.The results revealed that the activities of; G6PD (p˂0.01), 6PGD (p˂0.01), GR(p˂0.001) and GST (p˃0.05) enzyme activities were decreased in the CCl4group compared to the control group. It was determined that enzyme activities were higher in CCl4+Qu applied groups compared to CCl4group.The application of Qu caused an increase in the enzyme activity value. This can be accepted as an indication that the inhibition caused by CCI4has disappeared. Consequently; It is thought that Qu may be effective in preventing oxidative damage due to CCl4administration.

Keywords

• Carbon tetrachloride
• Quercetin
• Glucose 6-phosphate dehydrogenase
• 6-phosphogluconate dehydrogenase
• Glutathione reductase
• Glutatyon S-transferaz

Kaynakça

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