In VitroEffects of Some Drugs on Glucose-6-Phosphate Dehydrogenase Enzyme Purified from Sheep Spleen Tissue

Abstract

In this study, in vitroeffects of some drugs on glucose-6-phosphate dehydrogenase enzyme (G6PD; E.C. 1.1.1.49) purified from sheep spleen tissue were investigated. In the first step of the study, G6PD enzyme was purified from sheep spleen tissue by salting out(ammonium sulfateprecipitation) method and 2', 5' ADP-Sepharose 4B gel affinity chromatography and the purity of the enzyme was checked by SDS-PAGE method. In the second phase of the study, the effects of iveral, lincomycin, gentamicin, amoxicillin, ampicillin, streptomycin sulfate, novamizole, ketogesic, cefuroxime, cefazolin sodium and tylosin drugs on enzyme activity were investigated. Research results showed that iveral, gentamicin, streptomycin sulfate and lincomycin drugs inhibit the G6PD enzyme with IC50values of 0.62 mM, 21.6 mM, 173.2 mM and 231 mM, respectively, amoxicillin, ampicillin, novamizole, ketogenic, cefuroxime sodium, cefazolin sodium and tylosin drugs did not have a significant activation or inhibition effect on the enzyme.

Keywords

• Glucose 6-phosphate dehydrogenase
• Purification
• Drug
• Inhibition
• Iveral
• Gentamicin

Bazı İlaçların Koyun Dalak Dokusundan Saflaştırılan Glukoz-6-Fosfat Dehidrogenaz Enzimi Üzerine İn Vitro Etkileri

Öz

Bu çalışmada bazı ilaçların, koyun dalak dokusundan saflaştırılan glukoz-6-fosfat dehidrogenaz enzimi (G6PD; E.C. 1.1.1.49) üzerine in vitroetkileri araştırıldı. Çalışmanın ilk aşamasında G6PD enzimi koyun dalak dokusundan salting out(amonyum sülfat çöktürmesi) metodu ve 2', 5' ADP-Sepharose 4B afinite kromatografisi ile saflaştırıldı ve enzimin saflık derecesi SDS-PAGE metodu ile kontrol edildi. Çalışmanın ikinci aşamasında iveral, linkomisin, gentamisin, amoksisilin, ampisilin, streptomisin sülfat, novamizol, ketojezik, sefuroksim, sefazolin sodyum ve tylosin ilaçlarının enzim aktivitesi üzerindeki etkileri araştırıldı. Araştırma sonuçları; iveral, gentamisin, streptomisin sülfat ve linkomisin ilaçlarının G6PD enzimini sırasıyla 0,62 mM, 21,6 mM, 173,2 mM ve 231 mM, IC50değerleri ile inhibe ettiğini, amoksisilin, ampisilin, novamizol, ketojezik, sefuroksim sodyum, sefazolin sodyum ve tylosin ilaçlarının ise enzim üzerinde önemli bir aktivasyon ya da inhibisyon etkisine sahip olmadıklarını gösterdi.

Anahtar Kelimeler

• glukoz 6-fosfat dehidrogenaz
• saflaştırma
• ilaç
• inhibisyon
• iveral
• gentamisin

Kaynakça

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