Ratlara Malathion ve Rutin Uygulamaları Sonrası Akciğer Dokularında Metalloproteinaz Düzeyleri ile Oksidatif Stres ve Apoptoz Belirteçlerinin Değerlendirilmesi

Öz

Malathion (MLT), organofosfat sınıfında önemli bir çevresel kirleticidir. Rutin (RUT) ise çeşitli toksik ajanlara karşı etkinliği yoğun olarak araştırılan flavonoid aile üyelerinden biridir. Bu çalışmada, MLT ve RUT tedavilerinin sıçanların akciğer dokularında oksidatif stres, apoptoz ve metalloproteinazlar üzerine etkileri araştırıldı. Çalışmada, sıçanlara 28 gün MLT ve/veya RUT tedavisi verildikten sonra akciğer dokularındaki MDA, GSH, Nrf2, HO-1, MMP2, MMP9 ve kaspaz-3 seviyeleri biyokimyasal veya RT-PCR yöntemi ile analiz edildi. Veriler, MLT ile indüklenen MDA seviyelerinin RUT tedavisinden sonra düştüğünü gösterdi. Ayrıca Nrf2 ve HO-1 mRNA transkript düzeyleri ile MLT tarafından baskılanan GSH düzeylerinin RUT tedavisi sonrası kontrol grubu düzeylerine yaklaştığı belirlendi. MLT, akciğer dokularında metalloproteinazların (MMP2 ve MMP9) ekspresyonunu yukarı doğru düzenlerken, RUT bu genlerin ekspresyonunu aşağı regüle etti. Ayrıca MLT'nin kaspaz-3 ekspresyonunu tetiklediği, RUT'nin ise kaspaz-3'ü baskılayarak anti-apoptotik etki gösterdiği gözlendi. Sonuç olarak, MLT'nin oksidatif stres, apoptoz ve metalloproteinazlar yoluyla sıçanların akciğer dokularında toksik etkiler gösterirken, RUT'nin bu toksik etkileri azaltabileceği belirlendi.

Anahtar Kelimeler

• Akciğer
• Apoptoz
• Malathion
• Metalloproteinazlar
• Oksidatif stres
• Rutin

Evaluation of the Levels of Metalloproteinases as well as Markers of Oxidative Stress and Apoptosis in Lung Tissues After Malathion and Rutin Administrations to Rats

Abstract

Malathion (MLT) is an important environmental pollutant in the organophosphate class. Rutin (RUT), on the other hand, is one of the flavonoid family members whose effectiveness against various toxic agents has been extensively studied. In the present study, the effects of MLT and RUT treatments on oxidative stress, apoptosis and metalloproteinases in lung tissues of rats were investigated. In the study, MDA, GSH, Nrf2, HO-1, MMP2, MMP9 and caspase-3 levels in lung tissues were analyzed by biochemical or RT-PCR method after rats received MLT and/or RUT treatment for 28 days. The data showed that MLT-induced MDA levels decreased after RUT treatment. In addition, it was determined that Nrf2 and HO-1 mRNA transcript levels and GSH levels suppressed by MLT approached the control group levels after RUT treatment. MLT up-regulated the expression of metalloproteinases (MMP2 and MMP9) in lung tissues, while RUT down-regulated the expression of these genes. In addition, it was observed that MLT triggered caspase-3 expression, while RUT exerted an anti-apoptotic effect by suppressing caspase-3. As a result, it was determined that while MLT showed toxic effects in the lung tissues of rats through oxidative stress, apoptosis and metalloproteinases, RUT could alleviate these toxic effects.

Keywords

• Apoptosis
• Lung
• Malathion
• Metalloproteinases
• Oxidative stress
• Rutin

Kaynakça

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