Isolation of a Novel Amylase Producing Brachybacterium paraconglomeratum Strain FAD4 and Optimization of the Enzyme Production Conditions

Abstract

A novel amylase producing bacterium FAD4 was isolated from the wastewater of a textile factory located in Soke (Aydın/Turkey). The amylase production ability of gram positive, coccoidal FAD4 strain was confirmed with plate assay. Morphological and 16S rRNA sequence analyses revealed that FAD4 belongs to the Brachybacterium paraconglomeratum species with a sequence similarity of 99.8%. The optimal conditions for amylase production were determined as 72 h at 30 °C with supplementation of 1% starch. Optimum temperature and pH of the amylase were 50 °C and 7.0 respectively. Different starch, carbon and nitrogen sources were investigated for amylase production. A high enzyme production was observed with 1% potato starch and among nitrogen sources peptone was induced the production of amylase. Lactose, galactose, and fructose were also increased the enzyme production as carbon sources.

Keywords

• Amylase
• Brachybacterium
• starch

Kaynakça

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