Carvacrol, sodyum arsenit kaynaklı ince bağırsak toksisitesini iyileştirmektedir

Öz

Arsenik, doğal ve antropojenik faaliyetler yoluyla çevrede bol miktarda bulunan son derece tehlikeli bir metaldir. Bununla beraber doğal flavonoidlerle tedavinin arsenik toksisitesine karşı koruyucu etkisi olabileceği öngörülmektedir. Carvacrol (CRV) antioksidan, anti-inflamatuvar ve antiapoptotik özellikleri bilinen doğal bir fenolik bileşiktir. Bu çalışmanın amacı sodyum arsenit (SA) ile oluşturulan ince bağırsak toksisitesinde CRV’nin koruyucu etkilerinin araştırılmasıdır. Bu amaçla ratlar rastgele 5 gruba ayrıldı ve 14 gün boyunca oral olarak SA ve CRV uygulaması gerçekleştirildi. Veriler SA uygulaması ile lipid peroksidasyonunun şekillendiğini antioksidan enzim aktivitelerinin ise azaldığını göstermektedir. Antioksidan savunma durumundaki bu bozukluk CRV uygulaması ile azaldı. NF-κB ve IL-1β ekspresyon düzelerine bakıldığına SA uygulaması ile inflamasyonun şiddetlendiği CRV uygulaması ile bu şiddetin azaldığı tespit edildi. Buna ek olarak yine SA uygulanan ratlarda artan Caspase-3 ve Apaf1 ekspresyon düzeylerinin CRV uygulaması ile düştüğü ve apoptozun şiddetinin azaldığı belirlendi. Tüm bu veriler ışığında SA kaynaklı ince bağırsak toksisitesinde CRV’nin antioksidan, anti-inflamatuvar ve antiapoptotik etki göstererek dokuları hasarda koruduğu söylenebilir.

Anahtar Kelimeler

• Apoptoz
• Carvacrol
• Oksidatif stres
• Sodyum arsenit
• İnce bağırsak toksisitesi
• İnflammasyon

Carvacrol ameliorates sodium arsenite-induced intestinal toxicity

Abstract

Arsenic is a very dangerous metal that is widely distributed in the environment as a result of anthropogenic and natural processes. On the other hand, it is thought that treatment with natural flavonoids may protect against arsenic toxicity. Carvacrol (CRV) is a naturally occurring phenolic compound with anti-inflammatory, anti-apoptotic, and antioxidant properties. This study aims to investigate the protective effects of CRV on sodium arsenite (SA)-induced intestinal toxicity. For this purpose, rats were randomly divided into five groups and administered SA and CRV orally for 14 days. The data indicate that when SA is administered, lipid peroxidation increase and antioxidant enzyme activities decrease. The administration of CRV ameliorated this impairment in the antioxidant defense system. Examining the expression levels of NF-kB and IL-1 revealed that inflammation increased with SA application but decreased with CRV administration. Moreover, the expression levels of Caspase-3 and Apaf1 increased in rats treated with SA, whereas the severity of apoptosis decreased when CRV was administered. In light of these findings, it is possible to state that CRV protects tissues from damage by presenting antioxidant, anti-inflammatory, and anti-apoptotic effects in SA-induced intestinal toxicity.

Keywords

• Apoptosis
• Carvacrol
• Inflammation
• Oxidative stress
• Sodium arsenite
• Intestinal toxicity

Kaynakça

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