Araştırma Makalesi
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Yıl 2024, Cilt: 13 Sayı: 3, 62 - 66, 26.09.2024
https://doi.org/10.46810/tdfd.1507703

Öz

Kaynakça

  • Bhardwaj N, Kumar B, Agrawal K, Verma, P. Current perspective on production and applications of microbial cellulases: a review. Bioresour Bioprocess. 2021; 8:1-34.
  • Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: from lignocellulosic biomass to improved production. Energies. 2023;16(8):3598.
  • Ikegami W, Kamitakahara H, Teramoto Y, Takano T. Synthesis of optically inactive cellulose via cationic ring-opening polymerization. Cellulose. 2021;28(10):6125-6132.
  • Niranjan K, Ranganathan K, Yapa N. Isolation, characterization and identification of cellulase (Endo-β-1, 4-glucanase) producing bacteria from diverse locations. Vidyodaya Journal of Science. 2023; 26(01).
  • Liu L, Huang WC, Liu Y, Li M. Diversity of cellulolytic microorganisms and microbial cellulases. Int Biodeterior Biodegrad. 2021;163:105277.
  • Islam F, Roy, N. Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses. BMC Res Notes. 2018;11:1-6.
  • Kaur P, Taggar MS, Kaur J. Cellulolytic microorganisms: diversity and role in conversion of rice straw to bioethanol. Cell Chem Technol. 2020;54:613-34.
  • Bhagat SA, Kokitkar SS. Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production. J App Biol Biotech. 2021;9(6):154-161.
  • Malik WA, Javed S. Enhancement of cellulase production by cellulolytic bacteria SB125 in submerged fermentation medium and biochemical characterization of the enzyme. Int J Biol Macromol.2024;130415.
  • Hamdan NT, Jasim HM. Purification and characterization of cellulase enzyme from Trichoderma longibrachiatum isolated in Iraqi soil. IOSR J Biotechnol Biochem (IOSR-JBB). 2018;4:32-41.
  • Megha SV, Maragathavalli S, Brindha S, Karthikeyan V, Annadurai B, Gangwar SK. Isolation and purification of cellulase. Int J Sci. Nat.2019;6(3):474–479.
  • Vara S, Karnena MK. Fungal enzymatic degradation of industrial effluents–A review. Curr Res Environ Appl Mycol. 2020;10(1):417-442.
  • Verma N, Kumar V, Bansal MC. Valorization of waste biomass in fermentative production of cellulases: a review. Waste Biomass Valor. 2021;12:613-640.
  • Ejaz U, Sohail M, Ghanemi A. Cellulases: from bioactivity to a variety of industrial applications. Biomimetics. 2021;6(3):44.
  • Biçen HEI. Biochemical characterization of cellulase enzyme by Bacillus cereus ısolated from cellulotic waste [dissertation]. Turkey: University of Düzce; 2022.
  • Dikbaş N, Uçar S, Alım Ş. Purification of phytase enzyme from Lactobacillus brevis and biochemical properties. Biologia. 2023;78(9):2583-2591.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254.
  • Demir Y, Dikbaş N, Beydemir Ş. Purification and biochemical characterization of phytase enzyme from Lactobacillus coryniformis (MH121153). Mol Biotechnol. 2018; 60:783-790.
  • Balla A, Silini A, Cherif-Silini H, Bouket AC, Boudechicha A, Luptakova L, et al. Screening of cellulolytic bacteria from various ecosystems and their cellulases production under multi-stress conditions. Catalysts. 2020;12(7):769.
  • Inan Bektas K, Nalcaoğlu A, Ceylan E, Colak DN, Caglar P, Agirman S, et al. Isolation and characterization of detergent-compatible amylase-, protease-, lipase-, and cellulase-producing bacteria. Braz J Microbiol. 2023;54(2):725-737.
  • Ahmad T, Sharma A, Gupta G, Mansoor S, Jan S, Kaur B, et al. Response surface optimization of cellulase production from Aneurinibacillus aneurinilyticus BKT-9: An isolate of urban Himalayan freshwater. Saudi J Biol Sci. 2020;27(9):2333-2343.
  • An X, Zong Z, Zhang Q, Li Z, Zhong M, Long H, et al. Novel thermo-alkali-stable cellulase-producing Serratia sp. AXJ-M cooperates with Arthrobacter sp. AXJ-M1 to improve degradation of cellulose in papermaking black liquor. J Hazard Mater. 2022;421:126811.
  • Das T, Ali F, Rahman MS. Cellulase activity of a novel bacterial strain Arthrobacter woluwensis TDS9: Its application on bioconversion of paper mill sludge. J Genet Eng Biotechnol. 2022;20(1):87.
  • Krishnaswamy VG, Sridharan R, Kumar PS, Fathima MJ. Cellulase enzyme catalyst producing bacterial strains from vermicompost and its application in low-density polyethylene degradation. Chemosphere. 2022;288:132552.
  • Roy D, Gunri SK, Pal KK. Isolation, screening and characterization of efficient cellulose-degrading fungal and bacterial strains and preparation of their consortium under in vitro studies. 3 Biotech. 2024;14(5):1-15.
  • Biswas S, Saber MA, Tripty IA, Karim MA, Islam MA, Hasan MS, et al. Molecular characterization of cellulolytic (endo-and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh. Ann Microbiol. 2020;70:1-11.
  • Elsababty ZE, Abdel-Aziz SH, Ibrahim AM, Guirgis AA, Dawwam GE. Purification, biochemical characterization, and molecular cloning of cellulase from Bacillus licheniformis strain Z9 isolated from soil. J Genet Eng Biotechnol. 2022;20(1):34.
  • Anu Kumar S, Kumar A, Kumar V, Singh B. Optimization of cellulase production by Bacillus subtilis subsp. subtilis JJBS300 and biocatalytic potential in saccharification of alkaline-pretreated rice straw. Prep Biochem Biotechnol. 2021;51(7):697-704.
  • Malik WA, Javed S. Biochemical characterization of cellulase from Bacillus subtilis strain and its effect on digestibility and structural modifications of lignocellulose rich biomass. Front Bioeng Biotechnol. 2021;9:800265.
  • Shyaula M, Regmi S, Khadka D, Poudel RC, Dhakal A, Koirala D, et al. Characterization of thermostable cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil. Int J Microbiol. 2023;(1):3615757.
  • Dehghanikhah F, Shakarami J, Asoodeh A. Purification and biochemical characterization of alkalophilic cellulase from the symbiotic Bacillus subtilis BC1 of the leopard moth, Zeuzera pyrina (L.)(Lepidoptera: Cossidae). Curr Microbiol. 2020;77:1254-1261.
  • Shankar T, Sankaralingam S, Balachandran C, Chinnathambi A, Nasif O, Alharbi SA, et al. Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae). J King Saud Univ Sci. 2021;33(1):101261.
  • Fouda A, Alshallash KS, Atta HM, El Gamal MS, Bakry MM, Alawam AS, et al. Synthesis, optimization, and characterization of cellulase enzyme obtained from thermotolerant Bacillus subtilis F3: an ınsight into cotton fabric polishing activity. J Microbiol Biotechnol. 2024;34(1):207.

Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste

Yıl 2024, Cilt: 13 Sayı: 3, 62 - 66, 26.09.2024
https://doi.org/10.46810/tdfd.1507703

Öz

Cellulose, one of the most abundant carbohydrates on Earth, is a promising candidate for the production of second-generation biofuels such as ethanol and various everyday products. This polysaccharide is degraded by the enzyme cellulase, which is usually produced by microorganisms. Microbial cellulases are widely used in various industries (such as textiles, detergents, pharmaceuticals, food and paper).
In the present study, cellulase enzyme was partially purified from Bacillus pumilus ND8 strain isolated from garden waste and the pH and temperature values at which the enzyme showed optimum activity were determined. B. pumilus ND8 strain exhibited a cellulase activity of 8.6 U/mL as a result of partial purification and the protein concentration of the enzyme was measured as 6.72 mg/mL. The pH and temperature values at which the partially purified cellulase showed optimum activity were pH 5.5 and 60 °C, respectively. Km and Vmax values of the enzyme were determined as 0.81 mM and 14.2 µmol/min, respectively. In conclusion, cellulase purified from B. pumilus ND8 strain was found to possess unique properties that make it suitable for industrial applications.

Kaynakça

  • Bhardwaj N, Kumar B, Agrawal K, Verma, P. Current perspective on production and applications of microbial cellulases: a review. Bioresour Bioprocess. 2021; 8:1-34.
  • Ilić N, Milić M, Beluhan S, Dimitrijević-Branković S. Cellulases: from lignocellulosic biomass to improved production. Energies. 2023;16(8):3598.
  • Ikegami W, Kamitakahara H, Teramoto Y, Takano T. Synthesis of optically inactive cellulose via cationic ring-opening polymerization. Cellulose. 2021;28(10):6125-6132.
  • Niranjan K, Ranganathan K, Yapa N. Isolation, characterization and identification of cellulase (Endo-β-1, 4-glucanase) producing bacteria from diverse locations. Vidyodaya Journal of Science. 2023; 26(01).
  • Liu L, Huang WC, Liu Y, Li M. Diversity of cellulolytic microorganisms and microbial cellulases. Int Biodeterior Biodegrad. 2021;163:105277.
  • Islam F, Roy, N. Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses. BMC Res Notes. 2018;11:1-6.
  • Kaur P, Taggar MS, Kaur J. Cellulolytic microorganisms: diversity and role in conversion of rice straw to bioethanol. Cell Chem Technol. 2020;54:613-34.
  • Bhagat SA, Kokitkar SS. Isolation and identification of bacteria with cellulose-degrading potential from soil and optimization of cellulase production. J App Biol Biotech. 2021;9(6):154-161.
  • Malik WA, Javed S. Enhancement of cellulase production by cellulolytic bacteria SB125 in submerged fermentation medium and biochemical characterization of the enzyme. Int J Biol Macromol.2024;130415.
  • Hamdan NT, Jasim HM. Purification and characterization of cellulase enzyme from Trichoderma longibrachiatum isolated in Iraqi soil. IOSR J Biotechnol Biochem (IOSR-JBB). 2018;4:32-41.
  • Megha SV, Maragathavalli S, Brindha S, Karthikeyan V, Annadurai B, Gangwar SK. Isolation and purification of cellulase. Int J Sci. Nat.2019;6(3):474–479.
  • Vara S, Karnena MK. Fungal enzymatic degradation of industrial effluents–A review. Curr Res Environ Appl Mycol. 2020;10(1):417-442.
  • Verma N, Kumar V, Bansal MC. Valorization of waste biomass in fermentative production of cellulases: a review. Waste Biomass Valor. 2021;12:613-640.
  • Ejaz U, Sohail M, Ghanemi A. Cellulases: from bioactivity to a variety of industrial applications. Biomimetics. 2021;6(3):44.
  • Biçen HEI. Biochemical characterization of cellulase enzyme by Bacillus cereus ısolated from cellulotic waste [dissertation]. Turkey: University of Düzce; 2022.
  • Dikbaş N, Uçar S, Alım Ş. Purification of phytase enzyme from Lactobacillus brevis and biochemical properties. Biologia. 2023;78(9):2583-2591.
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-254.
  • Demir Y, Dikbaş N, Beydemir Ş. Purification and biochemical characterization of phytase enzyme from Lactobacillus coryniformis (MH121153). Mol Biotechnol. 2018; 60:783-790.
  • Balla A, Silini A, Cherif-Silini H, Bouket AC, Boudechicha A, Luptakova L, et al. Screening of cellulolytic bacteria from various ecosystems and their cellulases production under multi-stress conditions. Catalysts. 2020;12(7):769.
  • Inan Bektas K, Nalcaoğlu A, Ceylan E, Colak DN, Caglar P, Agirman S, et al. Isolation and characterization of detergent-compatible amylase-, protease-, lipase-, and cellulase-producing bacteria. Braz J Microbiol. 2023;54(2):725-737.
  • Ahmad T, Sharma A, Gupta G, Mansoor S, Jan S, Kaur B, et al. Response surface optimization of cellulase production from Aneurinibacillus aneurinilyticus BKT-9: An isolate of urban Himalayan freshwater. Saudi J Biol Sci. 2020;27(9):2333-2343.
  • An X, Zong Z, Zhang Q, Li Z, Zhong M, Long H, et al. Novel thermo-alkali-stable cellulase-producing Serratia sp. AXJ-M cooperates with Arthrobacter sp. AXJ-M1 to improve degradation of cellulose in papermaking black liquor. J Hazard Mater. 2022;421:126811.
  • Das T, Ali F, Rahman MS. Cellulase activity of a novel bacterial strain Arthrobacter woluwensis TDS9: Its application on bioconversion of paper mill sludge. J Genet Eng Biotechnol. 2022;20(1):87.
  • Krishnaswamy VG, Sridharan R, Kumar PS, Fathima MJ. Cellulase enzyme catalyst producing bacterial strains from vermicompost and its application in low-density polyethylene degradation. Chemosphere. 2022;288:132552.
  • Roy D, Gunri SK, Pal KK. Isolation, screening and characterization of efficient cellulose-degrading fungal and bacterial strains and preparation of their consortium under in vitro studies. 3 Biotech. 2024;14(5):1-15.
  • Biswas S, Saber MA, Tripty IA, Karim MA, Islam MA, Hasan MS, et al. Molecular characterization of cellulolytic (endo-and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh. Ann Microbiol. 2020;70:1-11.
  • Elsababty ZE, Abdel-Aziz SH, Ibrahim AM, Guirgis AA, Dawwam GE. Purification, biochemical characterization, and molecular cloning of cellulase from Bacillus licheniformis strain Z9 isolated from soil. J Genet Eng Biotechnol. 2022;20(1):34.
  • Anu Kumar S, Kumar A, Kumar V, Singh B. Optimization of cellulase production by Bacillus subtilis subsp. subtilis JJBS300 and biocatalytic potential in saccharification of alkaline-pretreated rice straw. Prep Biochem Biotechnol. 2021;51(7):697-704.
  • Malik WA, Javed S. Biochemical characterization of cellulase from Bacillus subtilis strain and its effect on digestibility and structural modifications of lignocellulose rich biomass. Front Bioeng Biotechnol. 2021;9:800265.
  • Shyaula M, Regmi S, Khadka D, Poudel RC, Dhakal A, Koirala D, et al. Characterization of thermostable cellulase from Bacillus licheniformis PANG L Isolated from the Himalayan Soil. Int J Microbiol. 2023;(1):3615757.
  • Dehghanikhah F, Shakarami J, Asoodeh A. Purification and biochemical characterization of alkalophilic cellulase from the symbiotic Bacillus subtilis BC1 of the leopard moth, Zeuzera pyrina (L.)(Lepidoptera: Cossidae). Curr Microbiol. 2020;77:1254-1261.
  • Shankar T, Sankaralingam S, Balachandran C, Chinnathambi A, Nasif O, Alharbi SA, et al. Purification and characterization of carboxymethylcellulase from Bacillus pumilus EWBCM1 isolated from earthworm gut (Eudrilus eugeniae). J King Saud Univ Sci. 2021;33(1):101261.
  • Fouda A, Alshallash KS, Atta HM, El Gamal MS, Bakry MM, Alawam AS, et al. Synthesis, optimization, and characterization of cellulase enzyme obtained from thermotolerant Bacillus subtilis F3: an ınsight into cotton fabric polishing activity. J Microbiol Biotechnol. 2024;34(1):207.
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Enzimler
Bölüm Makaleler
Yazarlar

Neslihan Dikbaş 0000-0001-9096-2761

Waleed Salih Salman Al Dahluz 0009-0002-9275-7328

Şeyma Alım 0000-0001-6684-7974

Sevda Uçar 0000-0002-3612-457X

Yayımlanma Tarihi 26 Eylül 2024
Gönderilme Tarihi 1 Temmuz 2024
Kabul Tarihi 29 Temmuz 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 13 Sayı: 3

Kaynak Göster

APA Dikbaş, N., Salih Salman Al Dahluz, W., Alım, Ş., Uçar, S. (2024). Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste. Türk Doğa Ve Fen Dergisi, 13(3), 62-66. https://doi.org/10.46810/tdfd.1507703
AMA Dikbaş N, Salih Salman Al Dahluz W, Alım Ş, Uçar S. Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste. TDFD. Eylül 2024;13(3):62-66. doi:10.46810/tdfd.1507703
Chicago Dikbaş, Neslihan, Waleed Salih Salman Al Dahluz, Şeyma Alım, ve Sevda Uçar. “Partial Purification and Biochemical Characterization of Cellulase from Bacillus Pumilus ND8 Isolated from Garden Waste”. Türk Doğa Ve Fen Dergisi 13, sy. 3 (Eylül 2024): 62-66. https://doi.org/10.46810/tdfd.1507703.
EndNote Dikbaş N, Salih Salman Al Dahluz W, Alım Ş, Uçar S (01 Eylül 2024) Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste. Türk Doğa ve Fen Dergisi 13 3 62–66.
IEEE N. Dikbaş, W. Salih Salman Al Dahluz, Ş. Alım, ve S. Uçar, “Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste”, TDFD, c. 13, sy. 3, ss. 62–66, 2024, doi: 10.46810/tdfd.1507703.
ISNAD Dikbaş, Neslihan vd. “Partial Purification and Biochemical Characterization of Cellulase from Bacillus Pumilus ND8 Isolated from Garden Waste”. Türk Doğa ve Fen Dergisi 13/3 (Eylül 2024), 62-66. https://doi.org/10.46810/tdfd.1507703.
JAMA Dikbaş N, Salih Salman Al Dahluz W, Alım Ş, Uçar S. Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste. TDFD. 2024;13:62–66.
MLA Dikbaş, Neslihan vd. “Partial Purification and Biochemical Characterization of Cellulase from Bacillus Pumilus ND8 Isolated from Garden Waste”. Türk Doğa Ve Fen Dergisi, c. 13, sy. 3, 2024, ss. 62-66, doi:10.46810/tdfd.1507703.
Vancouver Dikbaş N, Salih Salman Al Dahluz W, Alım Ş, Uçar S. Partial Purification and Biochemical Characterization of Cellulase from Bacillus pumilus ND8 Isolated from Garden Waste. TDFD. 2024;13(3):62-6.