Plantago Major L.'nin biyoaktif bileşen analizi ve in vitro antioksidan aktiviteleri

Öz

Plantago major L. (P. major) halk arasında tıbbi amaçlarla kullanılan bir bitkidir. Bu çalışmada, Muş'un Varto ilçesinde yetişen P. major bitkisinin tür tanımlaması yapıldıktan sonra etanol ekstraktı hazırlanmıştır. Fenolik içeriği ve miktarı HPLC ile belirlendi. Antioksidan aktiviteler için dört farklı yöntem kullanıldı. Bitkinin kimyasal içerik analizi sonucunda sırasıyla en yüksek miktarda Trans-p kumarik asit (89.0 ng/μL), Absisik asit (11.4 ng/μL), Salisilik asit (8.20 ng/μL) tespit edildi. Antioksidan sonuçlar değerlendirildiğinde, ekstraktın DPPH ve ABTS radikallerini standart antioksidanlara yakın bir güçle temizlediği gözlenmiştir. Aynı zamanda ekstrakt Fe+3 ve Cu+2 iyonlarını önemli ölçüde azaltmıştır. Örneklerin aktivitesinin genel olarak absorbans artışıyla birlikte arttığı tespit edilmiştir. Literatürde P. major'un antioksidan aktivitesi üzerine sınırlı sayıda çalışma olması bu çalışmayı daha da önemli kılmaktadır. Bu çalışmanın, bu türle ilgili gelecekte yapılacak araştırmalara ışık tutacağını umuyoruz.

Anahtar Kelimeler

• Antioksidan
• Serbest Radikal
• HPLC
• Fenolik
• P. major

Bioactive Component Analysis and in vitro Antioxidant Activities of Plantago Major L.

Abstract

Plantago major L. (P. major) is a plant used by people for medicinal purposes. In this study, ethanol extract was prepared after species identification of P. major plant growing in Varto district of Muş. Phenolic content and amount were determined by high pressure liquid chromatography (HPLC). Four different methods were used for antioxidant activities. As a result of the chemical content analysis of the plant, the highest amount of trans-p-cumaric acid (89.0 ng/μL), abscisic acid (11.4 ng/μL) and salicylic acid (8.20 ng/μL) were detected. When the antioxidant results were evaluated, it was observed that the extract scavenged DPPH and ABTS radicals with a power close to that of standard antioxidants. At the same time, the extract significantly reduced Fe+3 and Cu+2 ions. It was found that the activity of the samples generally increased with increasing absorbance. The fact that there are few studies on the antioxidant activity of P. major in the literature makes this study even more important. We hope that this study will shed light on future research on this species.

Keywords

• Antioxidant
• Free Radicals
• HPLC
• Phenolic
• P. major

Kaynakça

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