Bisfenol A ve türevlerinin oküler toksisitesinin Tavuk Yumurtası Testi - Koryoallantoik Membran (HET-CAM) testi ile değerlendirilmesi

Öz

Bisphenol A (BPA) ve türevleri olan Bisphenol F (BPF), Bisphenol S (BPS), insanları farklı şekillerde etkileyebilen endokrin bozucu kimyasallar olarak sınıflandırılmaktadır. Bu kimyasalların olumsuz sağlık etkileri hakkında endişeler dile getirilirken, literatürde göz tahrişine dair veri bulunmamaktadır. In vivo Draize testine alternatif bir yöntem olan HET-CAM testi ile BPA ve analoglarının karşılaştırmalı tahriş potansiyelini ilk kez ortaya koymak amaçlanmıştır. BPA, BPF ve BPS'nin tahrişi veya değişiklikleri, CAM yüzeyinde kan damarlarında kanama, pıhtılaşma veya lizis gibi olumsuz değişiklikler gözlemlenerek tespit edildi. Test sonuçları, BPA'nın görünmez bir damarla lizis ile karakterize orta düzeyde tahrişe neden olduğunu, BPS'nin ise kanama ve pıhtılaşma şeklinde görünen güçlü bir tahriş etkisine sahip olduğunu göstermektedir. BPF maruziyetinden sonra CAM yüzeyinde herhangi bir tahriş oluşmadı. Sonuç olarak, işçiler için daha yüksek olabilecek maruziyet dikkate alındığında, BPA ve BPS'nin insan sağlığı için ciddi toksik kimyasallar olduğu desteklenmiştir. Aynı zamanda, bu çalışma, özellikle endüstriyel maruziyetler bağlamında, bisfenollerin tahriş potansiyelini değerlendirmek için hayvan çalışmalarına alternatif bir yaklaşım sunduğu için önemlidir.

Anahtar Kelimeler

• Bisphenol
• toksisite
• irritasyon
• in vitro
• HET-CAM

Ocular toxicity assessment of Bisphenol A and its derivatives by The Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) assay

Abstract

Bisphenol A (BPA) and its derivatives, Bisphenol F (BPF), Bisphenol S (BPS) are classified as endocrine disruptor chemicals that can affect with the human in different ways. In raising concerns about the adverse health effects of these chemicals, no ocular irritation data is available in the literature. It was aimed to reveal the comparative irritation potential of BPA and its analogues for the first time with HET-CAM test, which is an alternative method to the in vivo Draize test. Irritancy or alterations of BPA, BPF and BPS were detected by observing adverse changes such as hemorrhage, coagulation, or lysis of blood vessels on the CAM surface. Test results indicate that BPA caused moderate irritation characterized by lysis with an invisible vein, whereas BPS had a strong irritation impact appearing as hemorrhage and coagulation. There was no irritancy after BPF exposure on the CAM surface. As a conclusion, it was supported that BPA and BPS serious toxic chemicals for human health when considering exposure that may be higher for workers. At the same time, this study is significant since it offers an alternative approach to animal studies for evaluating the irritation potential of bisphenols, particularly in the context of industrial exposures.

Keywords

• Bisphenol
• toxicity
• irritation
• in vitro
• HET-CAM

Kaynakça

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