Fluoksetinin deneysel verilmesinden sonra sıçan karaciğerinin histopatolojik incelenmesi

Abstract

Objective: To investigate the histopathological changes in the liver after experimental application of fluoxetine. Materials and methods: Nineteen male and 24 female rats of Sprague-Dawley strain were used to study the effect of administration of fluoxetine over a period of 3 weeks. The rats were randomly divided into five groups. Groups A to group D, each consisting of 10,10, 10, 7 rats respectively, were for different tests, while 6 rats were used as normal controls (N). For group A,B,C,D fluoxetine was administered via intraperitoneal enjection at a dosage of 5 mg/kg, 7.5 mg/kg, 10 mg/kg, 25 mg/kg, respectively. The experiment lasted 3 weeks. Hepatic tissue samples were extracted under anesthesia for histopathologic study. Liver tissues were fixed in 10% buffered formalin and embedded in paraffin. Histopathologic changes were evaluated on the H&E stained sections. Results: The major histopathological changes in the liver after fluoxetine are hydropic degeneration, karyomegaly, steatosis, lobuler inflammation, focal necrosis, apoptosis, disruption, twinning cell plates, cholestasis, portal area inflammation, Kupffer cell hyperplasia and double nucleus (P<0.05). Conclusion: Our results show that exposure of rats to fluoxetine leads to toxic effects. At the end of the exposure period, livers of all exposed rats, but no controls, showed hydropic degeneration, karyomegaly, steatosis, lobular inflammation, focal necrosis, apoptosis, disruption, apopitoz, ayrışma, çift hücre kordonları, kolestaz, portal twinning cell plates, cholestasis, portal area inflammation, alanda inflamasyon, Kupffer hücrelerinde hiperplazi ve Kupffer cell hyperplasia and double nucleus. The main histopathological changes in all exposed rats were histopatolojik değişiklik hepatosellüler hidropik vakuoler hepatocellular hydropic vacuolar degeneration (%100). dejenerasyondur (%100)

Keywords

• rat liver, fluoxetine, hepatotoxicity, degeneration, antidepressants

Histopathologic examination of rat liver after experimental application of fluoxetine

Öz

Amaç: Deneysel olarak fluoksetin verilmesinden sonra karaciğerdeki histopatolojik değişiklikleri araştırmak. Gereç ve yöntem: Sprague-Dawley cinsinden 19 dişi ve 24 erkek rata 3 hafta süresince verilen fluoksetinin etkileri incelendi. Ratlar rastgele 5 gruba ayrıldı. Grup A’dan D’ye kadar her grupta sırasıyla 10, 10, 10, 7 rat vardı. Her gruba farklı test uygulandı ve 6 rat normal kontrol grubu olarak kullanıldı (N). A, B, C, D gruplarına sırasıyla intraperitoneal enjeksiyon yoluyla 5 mg/kg, 7,5 mg/kg, 10 mg/kg, 25 mg/kg fluoksetin verildi. Deneyden 3 hafta sonra karaciğer örnekleri histopatolojik inceleme için anestezi altında alındı. Karaciğer dokuları %10’luk buffer formalinde tespit ettikten sonra parafine gömüldü. Histopatolojik değişiklikler H+E boyalı kesitlerde değerlendirildi. Sonuç: Fluoksetin verildikten sonra karaciğerdeki başlıca histopatolojik değişiklikler hidropik dejenerasyon, karyomegali, yağlanma, lobular inflamasyon, fokal nekroz, apopitoz, ayrışma, çift hücre kordonları, kolestaz, portal alanda inflamasyon, Kupffer hücrelerinde hiperplazi ve çift nukleusdu (p<0.05). Tartışma: Sonuçlarımız da ratların fluoksetine maruz kalması toksik etkilere yol açtığı görülmüştür. Deney sonunda, kontrol grubu dışında bütün maruz kalan ratların karaciğerlerinde hidropik dejenerasyon, karyomegali, yağlanma, lobular inflamasyon, fokal nekroz

Anahtar Kelimeler

• Rat karaciğeri, fluoksetin, hepatotoksik, dejenerasyon, antidepresanlar

Kaynakça

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31. Corresponding author
32. Kısmet Bildirici, MD
33. Akarbaşı Mah. Arısoy Sok. Ayseana Sitesi No:19 B Blok D:8 Eskisehir/ Turkey.
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