Meme Karsinomu Hücrelerinde Ekspresyonu Fenotipini Dansitometrik - Morfometrik - Yapısal Parametrelerle Tanımlamak: Samba 4000 ile Bir Görüntü Sitometresi ve Ardışık İkili Boyama Çalışması
Öz
Anahtar Kelimeler
Kaynakça
- Steeg PS. Bevilacqua G. Kopper L. eta. Evidence for
- a novel gene associated with low tumor metastatic
- potential. J Natl Gencer Inst 1003: 00: 200204.
- Stahl-JA. Leone A. Ftosengard AM. etal. Identification of a second human nm23 gene. nm23i-t2. Cancer Fteserch 1001: 40: 5555—5554.
- Urano T. Furul-rawa K. Shiku H. Expression of nm25iNDP kinase proteins on the cell supface. Oncogene 1003; 5: 13?1—13?5.
- Rcyds JA. Stephenson T.J. Hoes FtC. et al. Nm25 protein expression in ductal in situ and invasive breast carcinoma. J Natl Cancer Inst 1000; 02: 11004202.
- Hennessy C. Henry JA. May FE. st el. Expression of the antimetastatic gene nm23 in human breast cancer: an association with good prognosis. J Math Cancer Inst 1091; 03: 251—255.
- Türkiye Eiropatoioji Dergisi 100?;3 (3—4): P's-Y0
- ağa ...--. _- _=_ '_--_- .. ._ "_ 33535 i..:îıîîğjî .'-'
- In conclusion. karyometric parameters such as MUD.
- voo and GLD. measured by IBM.
- value to identify nm23 expression {non-metastatic are of important behavior} in the early stage of breast carcinoma. They
- may not also be expressed by morphcmetric parameters
- such as NA. NFF and DNA content. Further investiga—
- tions by combining immunocytochemical panels of
- well-known prognosticatcrs and image cytometric
- parameters seem to have an important roles in the
- evaluation of metastatic behavior. especially in No
- cases which are still under discussion for giving adjuvant chemotherapy.
- Barnes Ft. Masood 5. Barker E. et al. Low nrn25 protein expression In infiltrating ductal breast carcinomas correlates with reduced patient survival. Am J Pathe] 1001: 100: 245—250.
- ?. Setvan A. Lascus I. veron M. etal. NDP—Kinm25 expression in human breast cancer in relation to refaps. survival. and other prognostic factors: an immunohistochemical study. .J F'athol 1004: WE: 2?—54.
- Ftosengard AM. Krutxsch HC. Sheam A. et al. Reduced nm23rAwdprotein in tumor metastasis and aberrant Drosophila development. Nature 1559.- 342: Wi'-155.
- Steel M. Cancer genes: Compiexes and complexities. Lancet 1000: 342: ?'54—T551.
- Tokunga Y. Uma T. Furukawa K. et al. Ftaduced expression of nm23 H1. but not of nm23 H2. is concordant with the frequency of Iymphnode metastasis of human breast cancer. Int J Cancer t003: 55: 55—T1. T9
The Definition of nm 23 Protein Expression Phenotype in Breast Carcinoma Cells by Dansitometric - Morphometric - Textural Parameters : An Image Cytometric (ICM) and Consecutive - Double - Staining - (CDS) Study by Samba 4000
Öz
Bu çalışmanın amacı. nm23 protein ekspresyonun fenotipik özelliklerinin nükleus alanı. nükleusun şekil öeeliiği. DNA içeriği. kromatin heterojenitesi. DNA konsantrasyonu ve kromatin üniformitesi gibi kar— ycmetrik parametrelerle tanımlanıp tanımlanamaya— cağını araştırmaktır. nm23 protein reaktivitesinin sap— tanması için kullanılan immün sitokimya ve karyometrik parametrelerin ölçülmesi için kullanılan feulgen pararosanilin yöntemi ardışık olarak uygulandı. Üeğertendirmeler görüntü silornetresinde yapıldı. Meme kanseri hücrelerinde nükleus alani. nükleus şekil özelliği ve DNA içeriği ile nm23 reaktivitesi arasında ilişki anlamlı değildi. Krematin üniterrnitesi ve DNA konsantrasyonu nm23 poeitit hücrelerde negatif hücrelerdekine göre
Anahtar Kelimeler
Kaynakça
- Steeg PS. Bevilacqua G. Kopper L. eta. Evidence for
- a novel gene associated with low tumor metastatic
- potential. J Natl Gencer Inst 1003: 00: 200204.
- Stahl-JA. Leone A. Ftosengard AM. etal. Identification of a second human nm23 gene. nm23i-t2. Cancer Fteserch 1001: 40: 5555—5554.
- Urano T. Furul-rawa K. Shiku H. Expression of nm25iNDP kinase proteins on the cell supface. Oncogene 1003; 5: 13?1—13?5.
- Rcyds JA. Stephenson T.J. Hoes FtC. et al. Nm25 protein expression in ductal in situ and invasive breast carcinoma. J Natl Cancer Inst 1000; 02: 11004202.
- Hennessy C. Henry JA. May FE. st el. Expression of the antimetastatic gene nm23 in human breast cancer: an association with good prognosis. J Math Cancer Inst 1091; 03: 251—255.
- Türkiye Eiropatoioji Dergisi 100?;3 (3—4): P's-Y0
- ağa ...--. _- _=_ '_--_- .. ._ "_ 33535 i..:îıîîğjî .'-'
- In conclusion. karyometric parameters such as MUD.
- voo and GLD. measured by IBM.
- value to identify nm23 expression {non-metastatic are of important behavior} in the early stage of breast carcinoma. They
- may not also be expressed by morphcmetric parameters
- such as NA. NFF and DNA content. Further investiga—
- tions by combining immunocytochemical panels of
- well-known prognosticatcrs and image cytometric
- parameters seem to have an important roles in the
- evaluation of metastatic behavior. especially in No
- cases which are still under discussion for giving adjuvant chemotherapy.
- Barnes Ft. Masood 5. Barker E. et al. Low nrn25 protein expression In infiltrating ductal breast carcinomas correlates with reduced patient survival. Am J Pathe] 1001: 100: 245—250.
- ?. Setvan A. Lascus I. veron M. etal. NDP—Kinm25 expression in human breast cancer in relation to refaps. survival. and other prognostic factors: an immunohistochemical study. .J F'athol 1004: WE: 2?—54.
- Ftosengard AM. Krutxsch HC. Sheam A. et al. Reduced nm23rAwdprotein in tumor metastasis and aberrant Drosophila development. Nature 1559.- 342: Wi'-155.
- Steel M. Cancer genes: Compiexes and complexities. Lancet 1000: 342: ?'54—T551.
- Tokunga Y. Uma T. Furukawa K. et al. Ftaduced expression of nm23 H1. but not of nm23 H2. is concordant with the frequency of Iymphnode metastasis of human breast cancer. Int J Cancer t003: 55: 55—T1. T9
Toplam 24 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Kasım 1997 |
| Yayımlandığı Sayı | Yıl 1997 Cilt: 3 Sayı: 3-4 |