Pieris rapae L. Lepidoptera: Pieridae ’nin Erkek Üreme Sisteminin Anatomik-Histolojik Yapısı

Öz

Bu çalışmada ergin Pieris rapae’nin erkek üreme sistemi anatomik v e histolojik olarakincelenmiştir. Gözlemler ışık mikroskobu ile yapılmıştır. Histolojik çalışmalar parafinli gömmeortamındaki numunelerden 8µm’lik kesitler alınarak mikroskop altında yapılmıştır. Erkek P. rapae’ n i nüreme sistemi: lopsuz bir testis, bir çift vas deferens, bir çift vesikula seminalis, bir çift yardımcı bez,çift ejekülatör kanal, tek ejekülatör kanal ve kutikular simpleksten meydana gelmiş olup bu kısımlaranatomik olarak gözlenmiştir. P. rapae’ de testisin folikülleri anatomik olarak ayırt edilemez.Spermatogenezin çeşitli gelişme safhaları, vesikula seminalis ve ejekülatör kanal histolojik olarakgözlenmiştir

Anahtar Kelimeler

• Lepidoptera, Pieris rapae, üreme sistemi, anatomi, histoloji

The Anatomical And Histological Structure Of The Male Reproductive System Of Pieris rapae L. Lepidoptera: Pieridae

Abstract

In this study has examined the male internal reproductive system of adult Pieris rapaeanatomically and histologically. Observations have been made by means of light microscope taking8µm crosssections from samples in the paraffined embedding medium for the histological examination.The internal reproductive system of the maleP. rapaeconsists of the following: a testis without lobe, apair of vas deferens, a pair of vesicula seminalis, a pair of accessory glands, a pair of ejaculatory ducts,an ejaculator duct and cuticular simplex. These structures had been observed anatomically. Thefollicles in the testis cannot be distinguished anatomically. Various developmental phases of thespermatogenesis have been observed in the testis and ejaculator canal

Keywords

• Lepidoptera ,Pieris rapae, reproductive system, anatomy, histology

Kaynakça

1. Higgins LG, Rilley ND. Togfalter Europas und Nordwestafrikas, Hamburg und Berlin, 1971.
2. Demirsoy A. Entomoloji (Yaşamın Temel Kuralları Cilt II ,Kısım II). Ankara: Meteksan Basım Evi, 1997.
3. İren Z. Orta Anadolu Bölgesi’nde önemli bağ zararlılarının tespiti üzerine araştırmalar. Bitki Koruma Bülteni ;16: 201-222. Yel M. Ergin Pimpla turionellae(L.) (Hymenoptera: Ichneumonidae)’ nın erkek ve dişi üreme sisteminin anatomik – histolojik yapısı. Yüksek Lisans Tezi. Ankara, 1984.
4. De Bach P. Biological Control of Insect Pest and Weeds . London: Chapman and Hill Ltd, 1964.
5. Davey KG. Reproduction in the Insects. Edinburg and London: Oliver and boyd, 1965.
6. Chapman RF. The Insects: Structure and Function. London: English Language Book Society / Hodter and Stoughton, 1985.
7. Çanakçıoğlu H. Böceklerin Toplanma-Preperasyon Muhafaza ve Teşhisi. İstanbul: İstanbul Üniversitesi Orman Fakültesi, 1971.
8. Snodgrass RE. Principles of Insect Morpology. New York and London: Mc Graw Hill Book Company, inc, pp,1935.

9. Tuxen CL. Taxonomist’s glossary of Genitalia in Insects. London: J Jorgenson and Co, 1970.
10. Sekendiz OA. Entomoloji Çalışmalarında Histoloji Laboratuvarı Tekniği. Trabzon: Karadeniz Teknik Üniversitesi Orman Fakültesi, 1979.
11. Ozban N, Özmutlu Ö. Mikropreparasyon Yöntemleri. İstanbul, İ Ü Fen Fakültesi Basımevi, 1994.
12. Richards OV, Davies RG. Imms General Textbook of Entomology. London: Chapman and Hill Ltd, 1977.
13. Laird AK. A study of the types of male gonads found in the Acrididae (Orthoptera ) . J Morphol 1943; 77: 490.
14. Hinton HE. An inquiry into the natural classification of the Dryopoidae, based partly on a study of their internal anatomy. Trans Roy Entomol Soc 1939; 89: 133-134.
15. Rockstein M. The Physiology of Insecta . New-York and London: Academic Press ,Volume V, 648 p.p ,1973.
16. Kansu İ.A. Genel Entomoloji. Ankara, A Ü Ziraat Fak, 1990.
17. Callahan PS. Serial morphology as a technique for determination of reproductive patterns in the corn earworm, Heliothis zea (Boddie). Ann Entomol Soc Am 1958; 51: 413-421.
18. Wigglesworth V.B. The Principles of insect Physiology. London, Methuen and co. ltd, 1972.
19. Amaldoss G. Persistent occurrence of spermatogenesis in the adullt testes of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Proc Indian Acad Sci (Anim Sci) 1989d; 98: (6), 405-418.
20. Carson HL. A comparative study of the apical cell of the insect testis. J Morphology 1945; 77: 141-162.
21. Vurgun A. Ergin Agrotis segetum(Denis and Schiff.) (Lepidoptera: Noctuidae)’un erkek ve dişi iç üreme sisteminin anatomik – histolojik yapısı. Ankara, Yüksek Lisans Tezi, 1996.
22. Gillott C. Entomology. New York, Plenum press, 1980.
23. Sharma R, Sareen ML. Morphology, cytochemistry and role of apical cells in the testis of butterfly , Danaus chrisippus(L.) (Lepitoptera: Danaidae). Research – Bulletin of the Panjab Universty Science 1989; 40: (I-II), 84.
24. Callahan PS, Cascio T. Histology of the reproductive tracts and transmission of sperm in the corn earworm, Heliothis zea.Ann Entomol Soc Am 1963; 56: 535-556.
25. Callahan PS, Chapin JB. Morpology of the reproductive system and mating in two representative members of the family Noctuidae Pseudalatia unipuncta and Peridroma margarittosa, with comparison to Heliothis zea.Ann Entomol Soc Am 1960; 53: 763-782.
26. Buntin GD, Pedigo LP. Morphology of the male and famale reproductive systems of Plathypera scabra (F.) (Lepidoptera: Noctuidae). J The Kansas Entomol Soc 1983; 56(3): 377-386.
27. Leopold RA. The role of male accessory glands in insect reproduction. Ann Rev of Entomol 1976; 21: 221.
28. Amaldoss G., Fine structure with regard to sperm functions of the ductus ejeculatoris dublex of the male reproductive tract of Heliothis armigera (HÜbner) (Lepidoptera: Noctuidae). Proc Indian Acad Sci (Anim Sci ) b; 98 (1):1-13.
29. Babu PBS. Development and differantion of male reproductive organs in Opisina arenasella (Walker). Entomon 1995; 20(2) : 59-66.
30. Fanger H, Nauman CM. Correlation between the mesodermal male genital ducts and the spermatophore structure in a ditrysian mont, Zygaena trifolii(Esper 1783) (Lepidoptera: Zygaenidae). Acta Zoologica 1993;74: (3) 239-246.
31. Carroll AL. Interactions between body size and mating history influence the reproductive success of males of a tordricid moth, Zeiraphera canadensis, Canadian Journal of Zoology 1994; 72(12) : 2124-2132.
32. Hoque MR. Comparative morphology of the reproductive systems of Heliothis armigera H. punctigera(Wallengren) (Lepidoptera: Noctuidae). Bangladesh Journal of Zoology 1992; 20 (1) :17-26. (Hubner) and