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Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri

Yıl 2001, Cilt: 58 Sayı: 3, 81 - 86, 01.09.2001

Öz

Etken maddeleri, klorheksidin diglukonat, sanguinarin ekstrakt, setilpiridinium klorid, heksetidin, triklosan vetimol olan altı ticari ağız çalkalama suyunun in vitroantifungal aktiviteleri araştırıldı. Test organizması olarak çeşitliklinik örneklerden izole edilmiş Candida albicans,Saccharomyces cerevisiae, Candida parapsilosis, Candidaguilliermondii, Candida krusei, Candida tropicalis, Candida kefyr veCandida glabrata kullanıldı. Bu gargaralarınin vitroantifungal etkileri; MİK, MFK ve “kill-time” ları belirlenerek değerlendirildi. Tüm gargaraların MİK ve MFKdeğerleri arasında en fazla iki katlık bir fark olduğu, özellikle klorheksidin diglukonat'ın test edilen maya türlerine karşıdiğer gargaralara oranla çok daha düşük konsatrasyonlarda inhibitör ve fungisidal etki gösterdiği saptandı. Tüm testorganizmaları için en kısa “kill-time” a ≤15 saniye klorheksidin diglukonat ve setilpiridinium klorid’in, en uzun >180 saniye ise sanguinarin ekstrakt’ın sahip olduğu saptandı. Sonuç olarak klorheksidin diglukonat ve setilpiridinium klorid içeren gargaraların mayalara karşı in vitroolarakdaha etkili olduğu ancak in vivoetkilerinin klinik denemelerle desteklenmesi gerektiği düşünülmektedir

Kaynakça

  • 1. Giuliana G, Pizzo G, Milici ME, Musotto GC, Giangreco R. In vitro antifungal properties of mouthrinses contain ing antimicrobial agents. J Periodontol 1997;68(8):729-33.
  • 2. Ciancio SG. Use of mouthrinses for professional indications. J Clin Periodontol 1988;15(8):520-3.
  • 3. Overholser CD. Longitudinal clinical studies with antimicrobial mouthrinses. J Clin Periodontol 1988;15(8):517-9.
  • 4. Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C. Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol 1990;17(8): 575-9.
  • 5. Marsh PD. Microbiological aspects of the chemical control of plaque and gingivitis. J Dent Res 1992;71(7): 1431-8.
  • 6. Walker CB. Microbiological effects of mouthrinses containing antimicrobials. J Clin Periodontol 1988;15(8): 499-505.
  • 7. Barkvoll P, Attramadal A. Effects of nystatin and chlorhexidine digluconate on Candida albicans. Oral Surg Oral Med Oral Pathol 1989;67(3): 279-81.
  • 8. Zegarelli DJ. Fungal infections of the oral cavity. Otolaryngol Clin North Am 1993;26(6):1069-89.
  • 9. Fotos PG, Vincent SD, Hellstein JW. Oral candidosis. Clinical, historical, and therapeutic features of 100 cases. Oral Surg Oral Med Oral Pathol 1992;74(1):41-9.
  • 10. Listgarten M, Lai CH, Young V. Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. J Periodontol 1993;64(3):155-61.
  • 11. MacNeill S, Rindler E, Walker A, Brown AR, Cobb CM: Effects of tetracycline hydrochloride and chlorhexidine gluconate on Candida albicans. An in vitro study. J Clin Periodontol 1997;24(10):753-60.
  • 12. Gonzalez S, Lobos I, Guajardo A, Celis A, Zemelman R, Smith CT, Saglie FR. Yeasts in juvenile periodontitis. Preliminary observations by scanning electron microscopy. J Periodontol 1987;58(2):119-24.
  • 13. Laine P, Meurman JH, Murtomaa H, Lindqvist C, Torkko H, Pyrhonen S, Teerenhovi L. One-year trial of the effect of rinsing with an amine fluoride-stannous-fluoride-containing mouthwash on gingival index scores and salivary microbial counts in lymphoma patients receiving cytostatic drugs. J Clin Periodontol 1993;20(9):628-34.
  • 14. National Committee for Clinical Laboratory Standarts. 1997. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standart. NCCLS Document M27-A. 17(9):940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087.
  • 15. Thurmond JM, Brown AT, Sims RE, Ferretti GA, Raybould TP, Lillich TT, Henslee PJ. Oral Candida albicans in bone marrow transplant patients given chlorhexidine rinses: occurrence and susceptibilities to the agent. Oral Surg Oral Med Oral Pathol 1991;72(3):291-5.
  • 16. Pizzo G, Giuliana G. Antifungal activity of chlorhexidine containing mouthrinses. An in vitro study. Minerva Stomatol 1998;47(12):665-71.
  • 17. Epstein JB, Vickars L, Spinelli J, Reece D. Efficacy of chlorhexidine and nystatin rinses in prevention of oral complications in leukemia and bone marrow transplantation. Oral Surg Oral Med Oral Pathol 1992;73(6):682-9.
  • 18. Ciancio S. Expaned and future uses of mouthrinses. J Am Dent Assoc 1994;125 Suppl 2:29-32.
  • 19. Addy M, Hunter L. The effects of a 0.2% chlorhexidine gluconate mouthrinse on plaque, toothstaining and candida in aphthous ulcer patients. A double-blind placebo-controlled cross-over study. J Clin Periodontol 1987;14(5):267-73.
  • 20. Spijkervet FK, van Saene JJ, van Saene HK, Panders AK, Vermey A, Fidler V. Chlorhexidine inactivation by saliva. Oral Surg Oral Med Oral Pathol 1990;69(4):444-9.
  • 21. Slots J, Rams TE, Listgarten MA. Yeasts, enteric rods and pseudomonads in the subgingival flora of severe adult periodontitis. Oral Microbiol Immunol 1988;3(2):47-52.

The In Vitro Antifungal Activities Of Different Mouthrinses

Yıl 2001, Cilt: 58 Sayı: 3, 81 - 86, 01.09.2001

Öz

In this study, we aimed to investigate the in vitro antifungal activities of six different commercial mouthrinses;chlorhexidine digluconate, sanguinarine extract, cetylpyridinium chloride, hexetidine, triclosan and thymol. Wetested these agents activities against clinical isolates of Candida albicans, Saccharomyces cerevisiae, Candidaparapsilosis, Candida guilliermondii, Candida krusei, Candida tropicalis, Candida kefyrand Candida glabrata. Thein vitro activities of these agents were evaluated by determining the MIC and MFC of each product. The kill time ofeach drug was also determined. The difference between MIC and MFC values of six agents was not higher than twofold. It was found that chlorhexidine digluconate had inhibitory and fungicidal properties at lowest concentration whencompared to other products. While the kill-time of chlorhexidine digluconate and cetylpyridinium chloride was equalor less than 15 seconds, period > 180 seconds was required for sanguinarine extract.In conclusion, although all antimicrobial mouthrinses included in this study possess in vitroantifungalproperties, chlorhexidine digluconate and cetylpyridinium chloride are more active than other products tested. Thein vivoactivities of these products need to be supported by further clinical trials

Kaynakça

  • 1. Giuliana G, Pizzo G, Milici ME, Musotto GC, Giangreco R. In vitro antifungal properties of mouthrinses contain ing antimicrobial agents. J Periodontol 1997;68(8):729-33.
  • 2. Ciancio SG. Use of mouthrinses for professional indications. J Clin Periodontol 1988;15(8):520-3.
  • 3. Overholser CD. Longitudinal clinical studies with antimicrobial mouthrinses. J Clin Periodontol 1988;15(8):517-9.
  • 4. Overholser CD, Meiller TF, DePaola LG, Minah GE, Niehaus C. Comparative effects of 2 chemotherapeutic mouthrinses on the development of supragingival dental plaque and gingivitis. J Clin Periodontol 1990;17(8): 575-9.
  • 5. Marsh PD. Microbiological aspects of the chemical control of plaque and gingivitis. J Dent Res 1992;71(7): 1431-8.
  • 6. Walker CB. Microbiological effects of mouthrinses containing antimicrobials. J Clin Periodontol 1988;15(8): 499-505.
  • 7. Barkvoll P, Attramadal A. Effects of nystatin and chlorhexidine digluconate on Candida albicans. Oral Surg Oral Med Oral Pathol 1989;67(3): 279-81.
  • 8. Zegarelli DJ. Fungal infections of the oral cavity. Otolaryngol Clin North Am 1993;26(6):1069-89.
  • 9. Fotos PG, Vincent SD, Hellstein JW. Oral candidosis. Clinical, historical, and therapeutic features of 100 cases. Oral Surg Oral Med Oral Pathol 1992;74(1):41-9.
  • 10. Listgarten M, Lai CH, Young V. Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. J Periodontol 1993;64(3):155-61.
  • 11. MacNeill S, Rindler E, Walker A, Brown AR, Cobb CM: Effects of tetracycline hydrochloride and chlorhexidine gluconate on Candida albicans. An in vitro study. J Clin Periodontol 1997;24(10):753-60.
  • 12. Gonzalez S, Lobos I, Guajardo A, Celis A, Zemelman R, Smith CT, Saglie FR. Yeasts in juvenile periodontitis. Preliminary observations by scanning electron microscopy. J Periodontol 1987;58(2):119-24.
  • 13. Laine P, Meurman JH, Murtomaa H, Lindqvist C, Torkko H, Pyrhonen S, Teerenhovi L. One-year trial of the effect of rinsing with an amine fluoride-stannous-fluoride-containing mouthwash on gingival index scores and salivary microbial counts in lymphoma patients receiving cytostatic drugs. J Clin Periodontol 1993;20(9):628-34.
  • 14. National Committee for Clinical Laboratory Standarts. 1997. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standart. NCCLS Document M27-A. 17(9):940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087.
  • 15. Thurmond JM, Brown AT, Sims RE, Ferretti GA, Raybould TP, Lillich TT, Henslee PJ. Oral Candida albicans in bone marrow transplant patients given chlorhexidine rinses: occurrence and susceptibilities to the agent. Oral Surg Oral Med Oral Pathol 1991;72(3):291-5.
  • 16. Pizzo G, Giuliana G. Antifungal activity of chlorhexidine containing mouthrinses. An in vitro study. Minerva Stomatol 1998;47(12):665-71.
  • 17. Epstein JB, Vickars L, Spinelli J, Reece D. Efficacy of chlorhexidine and nystatin rinses in prevention of oral complications in leukemia and bone marrow transplantation. Oral Surg Oral Med Oral Pathol 1992;73(6):682-9.
  • 18. Ciancio S. Expaned and future uses of mouthrinses. J Am Dent Assoc 1994;125 Suppl 2:29-32.
  • 19. Addy M, Hunter L. The effects of a 0.2% chlorhexidine gluconate mouthrinse on plaque, toothstaining and candida in aphthous ulcer patients. A double-blind placebo-controlled cross-over study. J Clin Periodontol 1987;14(5):267-73.
  • 20. Spijkervet FK, van Saene JJ, van Saene HK, Panders AK, Vermey A, Fidler V. Chlorhexidine inactivation by saliva. Oral Surg Oral Med Oral Pathol 1990;69(4):444-9.
  • 21. Slots J, Rams TE, Listgarten MA. Yeasts, enteric rods and pseudomonads in the subgingival flora of severe adult periodontitis. Oral Microbiol Immunol 1988;3(2):47-52.
Toplam 21 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

Abdullah Kılıç Bu kişi benim

M. Ali Saraçlı Bu kişi benim

Mustafa Özyurt Bu kişi benim

Işıl Saygun Bu kişi benim

A. Celal Başustaoğlu Bu kişi benim

Yayımlanma Tarihi 1 Eylül 2001
Yayımlandığı Sayı Yıl 2001 Cilt: 58 Sayı: 3

Kaynak Göster

APA Kılıç, A., Saraçlı, M. A., Özyurt, M., Saygun, I., vd. (2001). Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri. Türk Hijyen Ve Deneysel Biyoloji Dergisi, 58(3), 81-86.
AMA Kılıç A, Saraçlı MA, Özyurt M, Saygun I, Başustaoğlu AC. Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri. Turk Hij Den Biyol Derg. Eylül 2001;58(3):81-86.
Chicago Kılıç, Abdullah, M. Ali Saraçlı, Mustafa Özyurt, Işıl Saygun, ve A. Celal Başustaoğlu. “Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri”. Türk Hijyen Ve Deneysel Biyoloji Dergisi 58, sy. 3 (Eylül 2001): 81-86.
EndNote Kılıç A, Saraçlı MA, Özyurt M, Saygun I, Başustaoğlu AC (01 Eylül 2001) Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri. Türk Hijyen ve Deneysel Biyoloji Dergisi 58 3 81–86.
IEEE A. Kılıç, M. A. Saraçlı, M. Özyurt, I. Saygun, ve A. C. Başustaoğlu, “Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri”, Turk Hij Den Biyol Derg, c. 58, sy. 3, ss. 81–86, 2001.
ISNAD Kılıç, Abdullah vd. “Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri”. Türk Hijyen ve Deneysel Biyoloji Dergisi 58/3 (Eylül 2001), 81-86.
JAMA Kılıç A, Saraçlı MA, Özyurt M, Saygun I, Başustaoğlu AC. Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri. Turk Hij Den Biyol Derg. 2001;58:81–86.
MLA Kılıç, Abdullah vd. “Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri”. Türk Hijyen Ve Deneysel Biyoloji Dergisi, c. 58, sy. 3, 2001, ss. 81-86.
Vancouver Kılıç A, Saraçlı MA, Özyurt M, Saygun I, Başustaoğlu AC. Ağız Çalkalama Sularının Gargara In Vitro Antifungal Aktiviteleri. Turk Hij Den Biyol Derg. 2001;58(3):81-6.