Disk Difüzyon ve E-Testi İle Meropenem, Ofloksasin Ve Sefepim'in Duyarlılıklarının Saptanması

Yıl 2004, Cilt: 61 Sayı: 1, 5 - 8, 01.06.2004

Abbas Yousefi Rad Ahmet Arslantürk

Öz

Hastanemizin poliklinik ve yatan hastalarından gönderilen balgam, bronkoalveolar lavaj, parasentez, vb.örneklerden izole edilen Pseudomonas aeruginosa n=15 , Klebsiella pneumoniae n=11 ve Escherichia coli n=5 suşlarının meropenem, sefepim ve ofloksasin’e karşı duyarlılıkları E-testi ve disk difüzyon yöntemleri ileölçüldü. Buna göre, E-testi ile Pseudomonas aeruginosameropeneme %73, ofloksasine %53, sefepime %67,Klebsiella pneumoniaesırasıyla %100, %91, %82 ve Escherichia coli her üç antibiyotiğe %60 duyarlı bulunmuştur.Disk difüzyon yöntemi ile Pseudomonas aeruginosameropeneme %73, ofloksasine %47, sefepime %53,Klebsiella pneumoniaesırasıyla %100, %91, %82 ve Escherichia coliher üç antibiyotiğe %80 duyarlı bulunmuştur

Anahtar Kelimeler

Hassasiyet testi, Gram negatif bakteriler, Meropenem, Sefepim, Ofloksasin

Determination Of Susceptibility Of Meropenem, Ofloxacin, Cefepime With Disc Diffusion And E-Test

Öz

The sensitivities of some of Pseudomonas aeruginosa n=15 , Klebsiella pneumoniae n=11 and Escherichiacoli n=5 isolated from the sputum, bronchoalveolar lavage, paracentesis fluid specimen etc., sent from patients ofour hospitals clinic and hospitalized patients, were measured against meropenem, cefepim and ofloxacin by E-Testand disc diffusion methods. According to this, it's found that on the E-Test, Pseudomonas aeruginosais sensitive73% to meropenem 53% to ofloxacin, 67% to cefepim, Klebsiella pneumoniaewas sensitive 100%, 91%, 82%and Escherichia coliwas sensitive 60% to each three antibiotics. By the method of disc diffusion, it is foundthat Pseudomonas aeruginosawas sensitive 73% to meropenem, 47% to ofloxacin, 53% to cefepim, Klebsiellapneumoniaeis sensitive in order to 100%, 91%, 82% and Escherichia coliis sensitive %80 to each three antibiotics

Anahtar Kelimeler

Susceptibility test, Gram negative bacteria, Meropenem, Cefepim, Ofloxacin

Kaynakça

• Baker CN, Stocker SA, Culver DH, Thornsberry C. Comparison of the E-Test to agar dilution, broth microdilution and agar diffusion susceptibility testing techniques by using aspecial challenge set of bacteria. J Clin Microbiol 1991; : 533.
• Huang MB, Baker CN, Banerjee S, Tenover FC. Accuracy of the E-Test for determining antimicrobial susceptibility of staphylococci, enterococci, Campylobacter jejuni and Gram-negative bacteria resistant to antimicrobial agents. J Clin Microbiol 1992; 30: 3243.
• Bryskier A. New concepts in the field of cephalosporins; C3’ quaternary ammonium cephems (Group IV). J Clin Microbiol Infect 1997; 3 (Suppl 1): 1.
• Grassi GG, Grassi C. Cefepime: overview of activity in vivo and in vitro. J Antimicrob. Chemother 1993; 32 (Suppl B): 87.
• Hancock REW, Bellido R. Antibacterial in vitro activity of fourth generation cephalosporins. J Chemother 1996; : 31.
• Amyes SGB, Gemmell CG. Antibiotic resistance in bacteria. J Clin Microbiol 1992; 36: 4.
• Munshi MH, Sack DA, Haider K, Ahmed ZU, Rahaman MM, Morshed MG. Plasmid-mediated resistance to nalidixic acid in Shigella dysenteriae type 1. Lancet 1987; 2: 419.
• Wolfson JS, Hooper DC. The fluoroquinolones: structures, mechanisms of action and resistance, and spectra of activity in vitro. Antimicrob Agents Chemother 1985; 29: 581.
• Hikida M, Kawashima K, Yoshida M, Mitsuhashi S. Inactivation of new carbapenem antibiotics by dehydropeptidase-I from porcine and human renal cortex. J Antimicrob Chemother 1992; 30(2): 129-34.
• Bax RP, Bastain W, Featherstone A, Wilkinson DM, Hutchison M, Haworth SJ. The pharmacokinetics of meropenem in volunteers. J Antimicrob Chemother 1989; 24 (Suppl A): 311-20.
• Jacoby GA, Carreras I. Activities of beta-lactam antibiotics agaist E.coli strains producing extended-spectrum beta-lactamases. Antimicrob. Agents Chemo. 1989; 34: 855-62.
• Martinez LM, Ortega MC, Suaeez AI. Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of Coryneform bacteria. J Clin Microbiol 1995; 33: 1318.
• Ulusoy S, Arda B, Özer Ö, Çetin B, ve Özinel M.A. E-Test: Yeni bir antimikrobiyal duyarlılık testinin disk difüzyon yöntemi ile karşılaştırılması. Ankem dergisi 1995; 9(1): 85-9.
• Gebitekin C. Pseudom onas aeruginosasuşlarının seftazidim, piperasilin, imipenem ve meropenem antibiyotiklerine duyarlılıkları ve meropenem MİK değerlerinin E-test ile incelenmesi. (Uzmanlık tezi). Haydarpaşa Numune Hastanesi. İstanbul 1996.