BibTex RIS Kaynak Göster

Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri

Yıl 2009, Cilt: 66 Sayı: 1, 7 - 13, 01.03.2009

Öz

Amaç: Bu çalmada yurdumuzda çeitli hastalklarn geleneksel tedavisinde kullanlan kekik Thymus vulgaris , kimyon Cuminum cyminum ve mersin Myrtus communis bitkilerinden elde edilen yalarn Leishmania promastigotlarnn canll üzerine etkilerinin belirlenmesi amaçlanmtr. Yöntem: Bitkilerden elde edilen yalar 2/10 ile 1/100 arasnda deien oranlarda seyreltilerek çalmaya alnmtr. 2.107 promastigot/ml Leishmania solüsyonundan alnan 100’er l mikrotitrasyon plaklarna datlm ve üzerlerine farkl oranlarda RPMI ile seyreltilen bitkisel yalardan 100’ er l konularak 20 dakika beklenmitir. Ik mikroskobu altnda Thoma lamnda hareketli ve hareketsiz promastigotlar saylarak canllk yüzdeleri hesaplanmtr. Bulgular: Aratrlan bitkisel yalardan kekik ya en yüksek antileishmanial etkiyi gösterirken LD50= 1/1200 ml/ml , LD50 deeri mersin ya için 1/1800 ml/ml ve kimyon ya için 1/3000 ml/ml olarak tespit edilmitir. Sonuç: Leishmaniasis ülkemizde görülen önemli paraziter hastalklardan biridir. Leishmaniasisin tedavisinde yaanan zorluklar nedeniyle alternatif ilaç araylar gündemdedir. leri çalmalara gereksinim duyulmakla birlikte, bu çalmada denenen bitkisel yalardan özellikle kekik yann leishmaniasis tedavisinde kullanlabilecei düünülmektedir.

Kaynakça

  • Bailey MS, Lockwood DN. Cutaneous leishmaniasis.Clin Dermatol 2007; 25:203-11.
  • Sayg G. Temel Tbbi Parazitoloji. 2. Bask. Sivas: Esnaf Ofset Matbaas, 2002.
  • Ok ÜZ, Balcolu C, Taylan Özkan A, Özensoy S, Özbel Y. Leishmaniasis in Turkey. Acta Tropica 2002; 84:43-8.
  • Denton H, Mcgregor JC, Coombs GH. Reduction of antileishmanial pentavalent antimonial drugs by a parasitespeci c thiol-dependent reductase, TDR1. Biochem J 2004; 381(2): 405-12.
  • Sundar S, More DK, Singh MK. Failure of pentavalent antimony in visceral leishmaniasis in India: report from the centre of the Indian epidemic. Clin Infect Dis 2000; 31: 1104-7.
  • Netto EM, Merdsen PD, Lianos-Cuentas EA, Costa JM, Cuba CC, Barreto AC, Badaro R, Johnson WD, Jones TC. Long-term follow up of patients with Leishmania braziliensis infection and treated eith Glucantime. Trans R Soc Med Hyg 1990; 84(3): 367-70.
  • Hammarton TC, Mottram JC, Doerig C. The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation. Prog Cell Cycle Res 2003; 5: 91-101.
  • Pegg AE, McCann PP. Polyamine metabolism and function in mammalian cells and protozoans. Atlas of Science: Biochemistry 1988. 1: 11-8.
  • Beheshti M, Ghotbi S, Amirizade S. Therapeutic and adverse effects of glucantime used for treatment of cutaneous leishmaniasis. Shiraz E-Medical Journal 2007; 8(4).
  • Adinol LE, Bonventre PF, Pas MV, Eppstein DA. Synergistic effect of glucantime and a liposome-encapsulated muramyl dipeptide analog in therapy of experimental visceral leishmaniasis. Infect Immun 1985; 48(2): 409- 16.
  • .Pontin K, Ademar A, Filho DS, Santos FF, Silva MLA, Cunha WR, Nanayakkara NPD, Bastos JK, Albuquerque S. In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract. Parasitol Res 2008; 103:487-92.
  • .Wallace RJ. Symposium on ‘Plants as animal foods: a case of catch 22?’ Antimicrobial properties of plant secondary metabolites. Proceedings of the Nutrition Society 2004; 63: 621-9.
  • Chang ST, Chen PF, Chang SC. Antibacterial activity of leaf essential oils and their constituents from Cinnamomum osmophloeum. Journal of Ethnopharmacology 2001; 77: 123-7.
  • Elgayyar M, Draughon FA, Golden DA, Mount JR. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection 2001; 64:1019-24.
  • Shapiro S, Meier A, Guggenheim B. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiology and Immunology 1994; 9: 202-8.
  • Nakamura TU, Mendonço-Filho RR, Morgado-Diaz JA. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int 2006; 55: 99-105.
  • Croft SL, Coombs GH. Leishmaniasis-current chemotheraphy and recent advances in the seach for novel drugs. Trends Parasitol 2003; 19: 502-8.
  • Brenzan MA, Nakamura CV, Dias-Filho BP. Antileishmanial activity of crude extract and coumarin from Calophyllum brasiliense leaves against Leishmania amazonensis. Parasitol Res 2007; 101(3): 715-22.
  • Çeliksöz A, Sayg G, Özçelik S, Öztop AY, anlda T. Trimethoprim-sulphametaxole ve o axsasin’in Leishmania promastigotlarna in vitro etkilerinin aratrlmas. T Parazitol Derg 1998; 22(4):343-7.
  • Luize PS, Tiuman TS, Morello LG, Maza PK, UedaNakamura T, Dias Filho BP, Cortez, DAG, Mello JCP de, Nakamura CV. Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Revista Brasileira de Ciências Farmacêuticas 2005; 41(1): 85-94.
  • Davies CR, Kaye P, Croft SL, Sundar S. Leishmaniasis, New approaches to disease control. BMJ 2003; 326: 377- 82.
  • Weigle K, Saravia NG. Natural history, clinical evolution and the host-parasite interaction in new world cutaneous leishmaniasis. Clin Dermatol 1996; 14: 433-50.
  • Özçelik S, Sümer Z, Deerli S, Ozan F, Sökmen A. Sarmsak (Allium sativum) özütü skolosidal ajan olarak kullanlabilir mi?. T Parazitol Derg 2007; 31(4):318-21.
  • Polat, Z.A., Vural A, Ozan F, Ozcelik S, In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocul Pharmacol Ther 2008; 24(1): 8-14.
  • Tripathi SC, Singh SP, Dube S. Studies on antifungal properties of essential oil of Trachyspermum ammi (L.) sprague. Journal of Phytopathology 1985; 116:113-120.
  • Habtemariam. In vitro antileishmanial effects of antibacterial diterpenes from two Ethiopian Premna species: P. schimperi and P. oligotricha. BMC Pharmacology 2003; 3(6).
  • Cosentino S, Tuberoso CIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F. In vitro antimicrobial activity and chemical composition of Sardinian thymus essential oils. App Microbiol 1999; 29: 130-5.
  • Lacoste E, Chaumont JP, Mandin D, Plumel MM, Matos FJ. Antiseptic properties of essential oil of Lippia sidoides cham. aplication to the cutenous micro aria. Ann Pharm Fr 1996; 54(5): 228-30.
  • Robledo S, Osorio E, Munoz D, et al. In vitro antileishmanial activities of thymol and hemisynthetic derivatives. Antimic Ag Chem 2005; 49(4): 1652-5.
  • Asslani U, Vilma T. Chemical composition of Albanian thyme oil (Thymus vulgaris L.). J Ess Oil Res 2003; 15(3): 165-7.
  • Boyraz N, Koçak R. Baz bitki ekstraklarnn in-vitro antifungal etkileri. SÜ Ziraat Fakültesi Dergisi 2006; 20(38): 82–7.
  • Asha MK, Prashanth B, Murali B. Antihelmintic activity of essential oil of Ocimum sanctum and eugenol. Fitoterapia 2001; 72(6): 669–70.
  • Rosa MSS, Mendonça-Filho RR, Bizzo HR. Antileishmanial activity of a linalool-rich essential oil from Croton cajucara. Antimic Ag Chem 2003; 47(6): 1895-901.
  • Ata AD, Özçelik S, Sökmen A. Deri leishmanisisi etkenleri üzerine, Sivas yöresinde endemik ve endemik olmayanbitkilerin (özütlerinin) antileishmanial etkilerinin arat- rlmas. T Parazitol Derg 2003; 27(3):170-5.
  • Khalid FA, Abdalla NM, Mohomed HEO, Toum AM, Magzoub MMA, Ali MS. Treatment of cutaneous leishmaniasis with some local Sudanese plants (Neem, Garad & Garlic). T Parazitol Derg 2004; 28(3):129-32.
  • Karamenderes C, Khan S, Tekwani BL, Jacob MR, Khan IA. Antiprotozoal and antimicrobial activities of Centaurea species growing in Turkey. Pharmaceutical Biology 2006; 44(7): 534-539

In Vitro Antileishmanial Activity Of Essential Oils Obtained From Thyme Thymus Vulgaris , Cummin Cuminum Cyminum And Mersin Myrtus Communis Plants

Yıl 2009, Cilt: 66 Sayı: 1, 7 - 13, 01.03.2009

Öz

Objective: In this study, it is aimed to determine the in vitro antileishmanial activity of essential oils obtained from three different plants such as Thyme Thymus vulgaris , Cummin Cuminum cyminum and Mersin Myrtus communis that widely used in traditional medicine in our country. Method: Essential oils from these plants were diluted ranging from 2/10 to 1/100. 100 l of Leishmania promastigote solution 2.107 promastigot/ml dispensed in microtitration plate wells and 100 l plant essantial oil diluted in RPMI with different ratios was added onto, afterwards waited for 20 minutes. The motile and non-motile promastigotes were counted on Thoma slide under light microscope and percentage of cell viability was assessed.Results: The highest in vitro antileishmanial activity against promastigote forms of the parasite was obtained with Thyme oil LD50= 1/1200 ml/ml . LD50 doses of Cumin oil and Mersin oil were 1/ 1800 ml/ml and 1/3000 ml/ml, respectively. Conclusion: Because of the diffuculties of the treatment of leishmaniasis which is one of the most important parasitic diseases in Turkey, new drug researches are concerned, recently. In this study, we observed that particularly Thyme oil can be used for the treatment of Leishmaniasis, after further researches are performed.

Kaynakça

  • Bailey MS, Lockwood DN. Cutaneous leishmaniasis.Clin Dermatol 2007; 25:203-11.
  • Sayg G. Temel Tbbi Parazitoloji. 2. Bask. Sivas: Esnaf Ofset Matbaas, 2002.
  • Ok ÜZ, Balcolu C, Taylan Özkan A, Özensoy S, Özbel Y. Leishmaniasis in Turkey. Acta Tropica 2002; 84:43-8.
  • Denton H, Mcgregor JC, Coombs GH. Reduction of antileishmanial pentavalent antimonial drugs by a parasitespeci c thiol-dependent reductase, TDR1. Biochem J 2004; 381(2): 405-12.
  • Sundar S, More DK, Singh MK. Failure of pentavalent antimony in visceral leishmaniasis in India: report from the centre of the Indian epidemic. Clin Infect Dis 2000; 31: 1104-7.
  • Netto EM, Merdsen PD, Lianos-Cuentas EA, Costa JM, Cuba CC, Barreto AC, Badaro R, Johnson WD, Jones TC. Long-term follow up of patients with Leishmania braziliensis infection and treated eith Glucantime. Trans R Soc Med Hyg 1990; 84(3): 367-70.
  • Hammarton TC, Mottram JC, Doerig C. The cell cycle of parasitic protozoa: potential for chemotherapeutic exploitation. Prog Cell Cycle Res 2003; 5: 91-101.
  • Pegg AE, McCann PP. Polyamine metabolism and function in mammalian cells and protozoans. Atlas of Science: Biochemistry 1988. 1: 11-8.
  • Beheshti M, Ghotbi S, Amirizade S. Therapeutic and adverse effects of glucantime used for treatment of cutaneous leishmaniasis. Shiraz E-Medical Journal 2007; 8(4).
  • Adinol LE, Bonventre PF, Pas MV, Eppstein DA. Synergistic effect of glucantime and a liposome-encapsulated muramyl dipeptide analog in therapy of experimental visceral leishmaniasis. Infect Immun 1985; 48(2): 409- 16.
  • .Pontin K, Ademar A, Filho DS, Santos FF, Silva MLA, Cunha WR, Nanayakkara NPD, Bastos JK, Albuquerque S. In vitro and in vivo antileishmanial activities of a Brazilian green propolis extract. Parasitol Res 2008; 103:487-92.
  • .Wallace RJ. Symposium on ‘Plants as animal foods: a case of catch 22?’ Antimicrobial properties of plant secondary metabolites. Proceedings of the Nutrition Society 2004; 63: 621-9.
  • Chang ST, Chen PF, Chang SC. Antibacterial activity of leaf essential oils and their constituents from Cinnamomum osmophloeum. Journal of Ethnopharmacology 2001; 77: 123-7.
  • Elgayyar M, Draughon FA, Golden DA, Mount JR. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection 2001; 64:1019-24.
  • Shapiro S, Meier A, Guggenheim B. The antimicrobial activity of essential oils and essential oil components towards oral bacteria. Oral Microbiology and Immunology 1994; 9: 202-8.
  • Nakamura TU, Mendonço-Filho RR, Morgado-Diaz JA. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int 2006; 55: 99-105.
  • Croft SL, Coombs GH. Leishmaniasis-current chemotheraphy and recent advances in the seach for novel drugs. Trends Parasitol 2003; 19: 502-8.
  • Brenzan MA, Nakamura CV, Dias-Filho BP. Antileishmanial activity of crude extract and coumarin from Calophyllum brasiliense leaves against Leishmania amazonensis. Parasitol Res 2007; 101(3): 715-22.
  • Çeliksöz A, Sayg G, Özçelik S, Öztop AY, anlda T. Trimethoprim-sulphametaxole ve o axsasin’in Leishmania promastigotlarna in vitro etkilerinin aratrlmas. T Parazitol Derg 1998; 22(4):343-7.
  • Luize PS, Tiuman TS, Morello LG, Maza PK, UedaNakamura T, Dias Filho BP, Cortez, DAG, Mello JCP de, Nakamura CV. Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Revista Brasileira de Ciências Farmacêuticas 2005; 41(1): 85-94.
  • Davies CR, Kaye P, Croft SL, Sundar S. Leishmaniasis, New approaches to disease control. BMJ 2003; 326: 377- 82.
  • Weigle K, Saravia NG. Natural history, clinical evolution and the host-parasite interaction in new world cutaneous leishmaniasis. Clin Dermatol 1996; 14: 433-50.
  • Özçelik S, Sümer Z, Deerli S, Ozan F, Sökmen A. Sarmsak (Allium sativum) özütü skolosidal ajan olarak kullanlabilir mi?. T Parazitol Derg 2007; 31(4):318-21.
  • Polat, Z.A., Vural A, Ozan F, Ozcelik S, In vitro evaluation of the amoebicidal activity of garlic (Allium sativum) extract on Acanthamoeba castellanii and its cytotoxic potential on corneal cells. J Ocul Pharmacol Ther 2008; 24(1): 8-14.
  • Tripathi SC, Singh SP, Dube S. Studies on antifungal properties of essential oil of Trachyspermum ammi (L.) sprague. Journal of Phytopathology 1985; 116:113-120.
  • Habtemariam. In vitro antileishmanial effects of antibacterial diterpenes from two Ethiopian Premna species: P. schimperi and P. oligotricha. BMC Pharmacology 2003; 3(6).
  • Cosentino S, Tuberoso CIG, Pisano B, Satta M, Mascia V, Arzedi E, Palmas F. In vitro antimicrobial activity and chemical composition of Sardinian thymus essential oils. App Microbiol 1999; 29: 130-5.
  • Lacoste E, Chaumont JP, Mandin D, Plumel MM, Matos FJ. Antiseptic properties of essential oil of Lippia sidoides cham. aplication to the cutenous micro aria. Ann Pharm Fr 1996; 54(5): 228-30.
  • Robledo S, Osorio E, Munoz D, et al. In vitro antileishmanial activities of thymol and hemisynthetic derivatives. Antimic Ag Chem 2005; 49(4): 1652-5.
  • Asslani U, Vilma T. Chemical composition of Albanian thyme oil (Thymus vulgaris L.). J Ess Oil Res 2003; 15(3): 165-7.
  • Boyraz N, Koçak R. Baz bitki ekstraklarnn in-vitro antifungal etkileri. SÜ Ziraat Fakültesi Dergisi 2006; 20(38): 82–7.
  • Asha MK, Prashanth B, Murali B. Antihelmintic activity of essential oil of Ocimum sanctum and eugenol. Fitoterapia 2001; 72(6): 669–70.
  • Rosa MSS, Mendonça-Filho RR, Bizzo HR. Antileishmanial activity of a linalool-rich essential oil from Croton cajucara. Antimic Ag Chem 2003; 47(6): 1895-901.
  • Ata AD, Özçelik S, Sökmen A. Deri leishmanisisi etkenleri üzerine, Sivas yöresinde endemik ve endemik olmayanbitkilerin (özütlerinin) antileishmanial etkilerinin arat- rlmas. T Parazitol Derg 2003; 27(3):170-5.
  • Khalid FA, Abdalla NM, Mohomed HEO, Toum AM, Magzoub MMA, Ali MS. Treatment of cutaneous leishmaniasis with some local Sudanese plants (Neem, Garad & Garlic). T Parazitol Derg 2004; 28(3):129-32.
  • Karamenderes C, Khan S, Tekwani BL, Jacob MR, Khan IA. Antiprotozoal and antimicrobial activities of Centaurea species growing in Turkey. Pharmaceutical Biology 2006; 44(7): 534-539
Toplam 36 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Araştırma Makalesi
Yazarlar

Erdoğan Malatyalı Bu kişi benim

Semra Özçelik Bu kişi benim

Nevcihan Gürsoy Bu kişi benim

Yayımlanma Tarihi 1 Mart 2009
Yayımlandığı Sayı Yıl 2009 Cilt: 66 Sayı: 1

Kaynak Göster

APA Malatyalı, E., Özçelik, S., & Gürsoy, N. (2009). Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri. Türk Hijyen Ve Deneysel Biyoloji Dergisi, 66(1), 7-13.
AMA Malatyalı E, Özçelik S, Gürsoy N. Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri. Turk Hij Den Biyol Derg. Mart 2009;66(1):7-13.
Chicago Malatyalı, Erdoğan, Semra Özçelik, ve Nevcihan Gürsoy. “Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri”. Türk Hijyen Ve Deneysel Biyoloji Dergisi 66, sy. 1 (Mart 2009): 7-13.
EndNote Malatyalı E, Özçelik S, Gürsoy N (01 Mart 2009) Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri. Türk Hijyen ve Deneysel Biyoloji Dergisi 66 1 7–13.
IEEE E. Malatyalı, S. Özçelik, ve N. Gürsoy, “Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri”, Turk Hij Den Biyol Derg, c. 66, sy. 1, ss. 7–13, 2009.
ISNAD Malatyalı, Erdoğan vd. “Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri”. Türk Hijyen ve Deneysel Biyoloji Dergisi 66/1 (Mart 2009), 7-13.
JAMA Malatyalı E, Özçelik S, Gürsoy N. Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri. Turk Hij Den Biyol Derg. 2009;66:7–13.
MLA Malatyalı, Erdoğan vd. “Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri”. Türk Hijyen Ve Deneysel Biyoloji Dergisi, c. 66, sy. 1, 2009, ss. 7-13.
Vancouver Malatyalı E, Özçelik S, Gürsoy N. Kekik Thymus Vulgaris , Kimyon Cuminum Cyminum Ve Mersin Myrtus Communis Bitkilerinden Elde Edilen Yağların İn Vitro Antileishmanial Etkileri. Turk Hij Den Biyol Derg. 2009;66(1):7-13.