Kauçuk yapıda Foley idrar sondalarının sitotoksisitesinde çinko bileşiklerinin olası rolü

Yıl 2012, Cilt: 69 Sayı: 1, 21 - 30, 01.03.2012

Mehmet Kürşat Derici Hakan Büzkaya Ferat Şahin

Öz

Amaç: Doğal kauçuk yapıda olan sonda ve cerrahi eldivenlerin farklı hücreler üzerindeki sitotoksik etkileri olduğu bildirilmiştir. Bu etkilerin azaltılması amacıyla gümüş ya da polimerik hidrojel, silikon, politetrafloroetilen kaplamalar gibi değişik kaplama teknikleri geliştirilmiştir. Kaplama sondaların üretim aşamasında, kauçuk sondalara benzer şekilde hızlandırıcı olarak çinko bileşikleri kullanılmaktadır. Bu çalışmanın amacı, kauçuk sondaların in vitro sitotoksik etkilerini, silikon kaplanan kauçuk sondalar ile karşılaştırmak ve toksisite nedenini araştırmaktır.Yöntem: Doğal kauçuk, silikon kaplı kauçuk ve %100 silikon olmak üzere üç grupta sekiz farklı markadan 48 sondanın sitotoksik etkileri, fare bağ dokusu fibroblast hücre kültüründe L-929 nitel görüntüleme ve skorlama ve nicel MTT yöntemler ile araştırılmıştır. Sonda özütlerinde bulunan element düzeyleri Alevli Atomik Absorbsiyon Spektrofotometresi AAS ile incelenmiştir.Bulgular: Negatif kontrol olarak kullanılan standart polietilen ile karşılaştırıldığında, %100 silikon sondalardan elde edilen özütlerin uygulandığı hücrelerde % canlılık oranı %95,43 ± 5,39 değişmezken, doğal kauçuk %8,57 ± 0,54 ve silikon kaplı kauçuk sondalarda %21,0 ± 2,52 canlılığın anlamlı olarak azaldığı görülmüştür p

Anahtar Kelimeler

Sitotoksisite, idrar sondaları, kaplama, çinko bileşikleri

The possible role of Zinc compounds on the cytotoxicity of latex Foley urinary catheters

Öz

Objective: The cytotoxic effects of natural rubber gloves and urinary catheters on different cells have been earlier reported. In order to reduce these effects, various coating techniques such as silver or polymer hydrogel, silicone, polytetrafluoroethylene PTFE coatings have been developed. However, during manufacturing process of silicone-coated rubber catheters, similar to latex catheters, zinc compounds, which have cytotoxic effects are added to the natural rubber base. The aim of this study was to compare the in vitro cytotoxic effects of rubber catheters with the silicone-coated rubber and to investigate the causes of the toxic effect.Method: The cytotoxic effects of total 48 urinary catheters in three different structure natural rubber, silicone, silicone-coated rubber and 8 different brands were analysed in mouse connective tissue fibroblast cell culture L-929 by qualitative imaging and scoring and quantitative MTT methods. The levels of the elements in catheter extracts were examined in an Atomic Absorption Spectrophotometer AAS .Results: Compared with standard polyethylene used as a negative control, the extracts from 100% silicone did not change vitality of the fibroblast cells oranı %95,43 ± 5,39 değişmezken, doğal kauçuk %8,57 ± 0,54 ve silikon kaplı kauçuk sondalarda %21,0 ± 2,52 canlılığın anlamlı olarak azaldığı görülmüştür p

Anahtar Kelimeler

Cytotoxicity, urinary catheters, coating, zinc compounds

Kaynakça

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