Akut Gastroenteritli Çocuklarda Human Bocavirus DNA Varlığının Araştırılması

Yıl 2017, Cilt: 74 Sayı: 4, 261 - 268, 01.12.2017

Serhat Sirekbasan Kenan Midilli Yasemin Akın Pelin Demirci Sevgi Ergin Arif Kaygusuz

Öz

Amaç: Akut gastroenteritler, çocuklarda alt solunum yolu enfeksiyonlarından sonra en sık morbidite ve mortalite nedenidir. Beş yaşın altındaki çocuklarda dünyada her yıl bir milyar çocuk ishale yakalanmakta ve yaklaşık altı milyon çocuk ishal nedeni ile kaybedilmektedir. Gastroenterit etkenlerinin bilinmesi, doğru tanı ve etkin tedavi fırsatı sağlamasının yanı sıra antimikrobiyal tedavi gereken durumlarda antibiyotik seçimi için de yol gösterici olmaktadır. Bu çalışmada, hastanemize başvuran 0-5 yaş arası çocuklardaki akut gastroenteritlerde Human Bocavirus varlığı ve sıklığının araştırılması amaçlandı.Yöntem: Çalışmaya, hastanemize başvuran akut gastroenteritli 0-5 yaş arasındaki 101 çocuktan 42 kız, 59 erkek alınan dışkı örnekleri dahil edilmiştir. Human Bocavirus HBoV DNA’sı NP-1 gen bölgesine uygun primer dizilerinin kullanıldığı PCR yöntemi ile araştırılmıştır.Bulgular: İncelenen 101 örnekten 7 % 6,9 ’si HBoV pozitif olarak saptanmıstır. HBoV pozitif hastaların yaş ortalaması 1,5 1–2,5 yaş olup %71,4’ü iki yaş altıdır. 7 pozitif örnekten de ikisinde %28,6 birden fazla virus açısından HBoV ve Norovirus pozitiflik elde edilmiş ve eşzamanlı enfeksiyon varlığı düşünülmüştür. was considered. Conclusion: Studies conducted to investigate the role of HBoV in gastrointestinal system infections are importantfor treatment and epidemiological purposes. Further studies are required for the purpose of determining the role of HBoV in acute gastroenteritis infections. We believe that the present study of ours will contribute to the epidemiological studies since it is the very first research in this field

Anahtar Kelimeler

İnsan Bocavirusu, gastroenterit, çocuk, polimeraz zincir reaksiyonu, dışkı

Investigation of Human Bocavirus DNA in children with acute gastroenteritis

Öz

Objective: Acute gastroenteritis is the second frequent reason of morbidity and mortality after lower respiratory tract infections. One billion children below the age of five have diarrhea every year throughout the world, and nearly six million children are lost due to diarrhea. Knowing gastroenteritis factors enables physicians to make accurate and efficient diagnoses, and guides them to select the right antibiotic when the cases require antimicrobial treatment. In this study, the purpose is to examine the existence and frequency of Human Bocavirus in acute gastroenteritis in children between 0-5 years of age applying to our hospital.Methods: Stool samples taken from 101 children 42 female, 59 male between the age of 0-5 with acute gastroenteritis were included in the study. The DNA of the Human Bocavirus HBoV was investigated with the PCR Method in which proper primary series were used for NP-1 gen area. Results: Seven 6,9% of the 101 samples that were examined in the scope of the study were determined to be HBoV positive. The mean age of the patients who were HBoV positive was 1,5 1-2,5 years of age ; and 71,4% were below the age of two. In two of the 7 positive samples 28,6% positivity for more than more virus HBoV and Norovirus was determined, and simultaneous infection was considered. Conclusion: Studies conducted to investigate the role of HBoV in gastrointestinal system infections are importantfor treatment and epidemiological purposes. Further studies are required for the purpose of determining the role of HBoV in acute gastroenteritis infections. We believe that the present study of ours will contribute to the epidemiological studies since it is the very first research in this field

Anahtar Kelimeler

Human Bocavirus, gastroenteritis, child, polymerase chain reaction, stool

Kaynakça

• Albayrak N, Yağcı-Çağlayık D, Altaş AB, Korukluoğlu G, Ertek M. Refik Saydam Hıfzıssıhha Merkezi Başkanlığı, Viroloji Referans ve Araştırma Laboratuvarı, 2009 yılı akut viral gastroenterit verilerinin değerlendirilmesi. Türk Hij Den Biyol Derg, 2011; 68(1): 9-15.
• Akhter S, Türegün B, Kıyan M, Gerçeker D, Güriz H, Şahin F. Beş yaş altı çocuklarda gastroenterite neden olan yedi farklı RNA virusunun araştırılması. Mikrobiyol Bul, 2014; 48(2): 233-41.
• Allander T, Tammi MT, Eriksson M, Bjerkner A, Tiveljung-Lindell A, Andersson B. Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci USA. 2005; 102(36): 12891-6.
• Allander T. Human bocavirus. J Clin Virol, 2008; 41(1): 29-33.
• Arnold JC, Singh KK, Spector SA, Sawyer MH. Human bocavirus: prevalence and clinical spectrum at a children’s hospital. Clin Infect Dis, 2006; 43(3): 283-8.
• Vicente D, Cilla G, Montes M, Perez-Yarza EG, Perez- Trallero E. Human bocavirus, a respiratory and enteric virus. Emerg Infect Dis, 2007; 13(4): 636-7.
• Neske F, Blessing K, Tollmann F, Schubert J, Rethwilm A, Kreth HW, et al. Real-time PCR for diagnosis of human bocavirus infections and phylogenetic analysis. J Clin Microbiol, 2007; 45(7): 2116-22.
• Kesebir D, Vazquez M, Weibel C, Shapiro ED, Ferguson D, Landry ML, et al. Human bocavirus infection in young children in the United States: molecular epidemiological profile and clinical characteristics of a newly emerging respiratory virus. J Infect Dis, 2006; 194(9): 1276-82.
• Lau SKP, Yip CC, Que TL, Lee RA, Au-Yeung RK, Zhou B, et al. Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong. J Infect Dis, 2007; 196(7): 986-93.
• Arden KE, McErlean P, Nissen MD, Sloots TP, Mackay IM. Frequent detection of human rhinoviruses, paramyxoviruses, coronaviruses, and bocavirus during acute respiratory tract infections. J Med Virol, 2006; 78(9): 1232-40.
• Midilli K, Yılmaz G, Türkoğlu S, Iskanova B, Ergin S, Yarımcam F ve ark. Akut solunum yolu enfeksiyonlu çocuk ve erişkinlerde insan bokavirus DNA’sının polimeraz zincir reaksiyonu ile saptanması. Mikrobiyol Bul, 2010; 44: 405-13.
• Ziyade N, Şirin G, Elgörmüş N, Daş T. Detection of human bocavirus DNA by multiplex PCR analysis: postmortem case report. Balkan Med J, 2015; 32(2): 226-9.
• Yeşilbaş O, Kıhtır HS, Talip Petmezci M, Balkaya S, Hatipoğlu N, Meşe S, et al. Very rare and life-threatening complications of bocavirus bronchiolitis: pneumomediastinum and bilateral pneumothorax. Mikrobiyol Bul, 2016; 50(1): 159-64.
• Aktürk H, Sütçü M, Badur S, Törün SH, Çıtak A, Erol OB, et al. Evaluation of epidemiological and clinical features of influenza and other respiratory viruses. Turk Pediatri Ars, 2015; 50(4): 217-25.
• Mitui MT, Bozdayi G, Ahmed S, Matsumoto T, Nishizono A, Ahmed K. Detection and molecular characterization of diarrhea causing viruses in single and mixed infections in children: a comparative study between Bangladesh and Turkey. J Med Virol, 2014; 86(7): 1159-68.
• Albuquerque MC, Rocha LN, Benati FJ, Soares CC, Maranhao AG, Ramirez ML, et al. Human bocavirus infection in children with gastroenteritis, Brazil. Emerg Infect Dis, 2007; 13(11): 1756-8.
• Lee JI, Chung JY, Han TH, Song MO, Hwang ES. Detection of human bocavirus in children hospitalized because of acute gastroenteritis. J Infect Dis, 2007; 196(7): 994-7.
• Campe H, Hartberger C, Sing A. Role of human bocavirus infections in outbreaks of gastroenteritis. J Clin Virol, 2008; 43(3): 340-2.
• Szomor KN, Kapusinszky B, Rigo Z, Kis Z, Rozsa M, Farkas A, et al. Detection of human bocavirus from fecal samples of Hungarian children with acute gastroenteritis. Intervirology, 2009; 52(1): 17-21.
• Khamrin P, Malasao R, Chaimongkol N, Ukarapol N, Kongsricharoern T, Okitsu S, et al. Circulating of human bocavirus 1, 2, 3, and 4 in pediatric patients with acute gastroenteritis in Thailand. Infect Genet Evol, 2012; 12(3): 565-9.
• Romani S, Mohebbi SR, Khanyaghma M, Azimzadeh P, Bozorgi SM, Damavand B, et al. Detection of human bocavirus 1, 2 and 3 from patients with acute gastroenteritis. Gastroenterol Hepatol Bed Bench, 2013; 6(Suppl 1): 77-81.
• La Rosa G, Della Libera S, Iaconelli M, Donia D, Cenko F, Xhelilaj G, et al. Human bocavirus in children with acutegastroenteritis in Albania. J Med Virol, 2016; 88(5): 906-10.
• Cheng WX, Jin Y, Duan ZJ, Xu ZQ, Qi HM, Zhang Q, et al. Human bocavirus in children hospitalized for acute gastroenteritis: a case-control study. Clin Infect Dis, 2008; 47(2): 161-7.
• Yu JM, Li DD, Xu ZQ, Cheng WX, Zhang Q, Li HY, Cui SX, et al. Human bocavirus infection in children hospitalized with acute gastroenteritis in China. J Clin Virol, 2008; 42(3): 280-5.