İçme Kullanma Sularındaki Cryptosporidium Ookist ve Giardia Kistlerinin Analizinde Ts Iso 15553 Standart Yönteminin Uygulanabilirliğinin Dış Kalite Kontrol Numunesi İle Değerlendirilmesi

Yıl 2017, Cilt: 74 Sayı: EK-1, 7 - 12, 01.12.2017

Şule Şenses Ergül Pınar Kaynar Mustafa Yılmaz Yıldırım Cesaretli Mustafa Kemal Başarılı İrfan Şencan

Öz

Amaç: Bu çalışmanın amacı, içme-kullanma sularının analizinde Cryptosporidium ookist ve Giardia kistlerinin tespiti amacıyla TS ISO 15553 Standart yönteminin uygulanabilirliğinin dış kalite kontrol DKK numunesi ile belirlenmesidir. Yöntem: Çalışmada, bir adet dış kalite kontrol numunesi ile farklı zamanlarda analize alınan üç adet pozitif kalite kontrol numunesi, TS ISO 15553 Standart yöntemi kullanılarak analiz edilmiştir. Ayrıca her bir çalışmaya negatif kalite kontrol numunesi de dahil edilmiştir. Bulgular: Çalışmada, dış kalite kontrol numunesi ve pozitif kalite kontrol numuneleri ile edilen sonuçlar birlikte değerlendirildiğinde; TS ISO 15553 Standart yöntemi ile içme-kullanma sularındaki Cryptosporidium ookist ve Giaridia kistlerinin varlığı tespit edilmiştir. Sonuç: Çalışmada, DKK numunesi ile elde edilen sonuca göre TS ISO 15553 Standardının laboratuvarda analiz edilecek su numunelerinde uygulanabileceği gösterilmiştir.

Anahtar Kelimeler

Cryptosporidium ookist, Giardia kist, içme-kullanma suyu, TS ISO 15553, halk sağlığı

Evaluation of the Applicability of the Standard Method of Ts Iso 15553 in Analysis of Cryptosporidium Oocysts and Giardia Cysts in Drinking Waters Using External Quality Control Samples

Öz

Objective: The aim of the present study was to determine the applicability of TS ISO 15553 Standard method in water analyses in order to detect Cryptosporidium oocysts and Giardia cysts by using external quality control EQC sample. Methods: In the study, one external quality control sample and three positive quality control samples analysed at different intervals, were tested by using TS ISO 15553 standard method. Besides, negative quality control samples were also includued in each study design. Results: When the results obtained with external quality control and positive quality control samples were evaluated together, it was determined that the presence of Cryptosporidium oocysts and Giardia cysts could be detected in water-intended for human consumption by using TS ISO 15553 Standard method. Conclusion: According to the results obtained by using external quality control sample, it was confirmed that TS ISO 15553 Standard method can be used in the laboratory for analyzing water samples

Anahtar Kelimeler

Cryptosporidium oocysts, Giardia cysts, water inteded for human consumption, TS ISO 15553, public health

Kaynakça

• Gajadhar AA, Allen JR. Factors contributing to the public health and economic importance of waterborne zoonotic parasites. Vet Parasitol, 2004; 126: 3-14.
• Karanis P. A review of an emerging waterborne medical important parasitic protozoan. Jpn J Protozool, 2006; 39 (1): 5-19.
• Köksal F, Başlantı İ, Samastia M. Retrospective Evaluation of the Prevalence of Intestinal Parasites in Istanbul, Turkey. Türkiye Parazitol Derg, 2010; 34 (3): 166-71.
• Carmena D. Waterborne transmission of Cryptosporidium and Giardia: detection, surveillance and implications for public health. Formatex, 2010; 3-14.
• Anonymous. İnsani Tüketim Amaçlı Sular Hakkında Yönetmelik. Ankara: T.C. Sağlık Bakanlığı, 2005
• Anonymous, EPA Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, 2001.
• Anonymous, EPA Method 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA, 2005.
• Kerri LX, Alderisio A, Jiang J. Detection of cryptosporidium oocysts in water: effect of the number of samples and analytic replicates on test results. App Env Microbiol, 2006; 72(9): 5942-47.
• Anonymous. TS ISO 15553 Su Kalitesi - Cryptosporidium Ookist Ve Giardia Kistin Sudan Ayrılması ve Belirlenmesi. Ankara: Türk Standartları Enstitüsü, 2014.
• Skotarczak B. Methods for parasitic protozoans detection in the environmental samples. Parasite; 2009; 16: 183-90.
• Kumar T, Majid MAA, Onichandran S, Jaturas N, Andiappan H, Salibay CC, et al. Presence of cryptosporidium parvum and giardia lamblia in water samples from Southeast Asia: towards an integrated water detection system. Infect Dis Poverty, 2016; 5:1-12.
• Moss JA, Gordy J, Snyder RA. Effective concentration and detection of cryptosporidium, giardia, and the microsporidia from environmental matrices. J Pathog, 2014; 1-10.