Antibiofilm activities and in vitro susceptibility testing of eucalyptus (Eucalyptus camaldulensis) essential oil (EO) against fish pathogen Pseudomonas species

Öz

Essential oils are concoctions of aromatic and volatile chemicals extracted from several plant species. These mixes have been used by society for a variety of reasons, and they play significant functions in nature. This study aimed to analyze the biological properties of essential oil extracted from Eucalyptus camaldulensis leaves, including its antipseudomonal and antibiofilm effects. Antibiotics have been heavily used both to treat bacterial infections and to stimulate fish growth, which has led to the emergence of germs that are resistant to the drugs. The McFarland 0.5 standard was used to determine the study inoculums, and the standard disk diffusion technique was used to analyze the antimicrobial activity. The essential oils from E. camaldulensis possessed antibacterial activity against tested Pseudomonas aeruginosa, P. fluorescens, and P. putida at 5-10 μg/disc. The antibacterial effect was determined to be concentration dependent. Our findings showed that E. camaldulensis essential oil made an excellent source of antipseudomonal, and also exhibited inhibition of Pseudomonas species biofilm formation. Based on its antibacterial and antibiofilm potential, E. camaldulensis essential oil shows promise as a potential source of antibacterial agents. Therefore, the use of E. camaldulensis essential oil in applications may have the potential to be a natural antibacterial agent against pathogenic and spoiling microorganisms.

Anahtar Kelimeler

• antibacterial activity
• antibiofilm activity
• essential oil
• eucalyptus
• pseudomonas

Okaliptüs uçucu yağının bazı Pseudomonas türlerine karşı antibiyofilm aktiviteleri ve antimikrobiyal duyarlılığı

Öz

Uçucu yağlar, çeşitli bitki türlerinden ekstrakte edilen aromatik ve uçucu kimyasalların karışımlarıdır. Bu karışımlar toplum tarafından çeşitli amaçlarla kullanılmakta ve doğada önemli işlevler üstlenmektedirler. Bu çalışma, Eucalyptus camaldulensis yapraklarından ekstrakte edilen esansiyel yağın, antipsödomonal ve antibiyofilm etkileri de dahil olmak üzere biyolojik özelliklerini analiz etmeyi amaçlamıştır. Antibiyotikler hem bakteriyel enfeksiyonları tedavi etmek hem de balık büyümesini teşvik etmek için yoğun bir şekilde kullanılmış ve bu da ilaçlara dirençli mikropların ortaya çıkmasına neden olmuştur. Çalışma inokulumlarını belirlemek için McFarland 0.5 standardı, antimikrobiyal aktiviteyi analiz etmek içinse standart disk difüzyon tekniği kullanıldı. E. camaldulensis'ten elde edilen uçucu yağlar, test edilen Pseudomonas aeruginosa, P. fluorescens ve P. putida'ya karşı 5-10 μg/disk seviyesinde antibakteriyel aktivite gösterdi. Antibakteriyel etkinin konsantrasyona bağlı olduğu belirlendi. Bulgularımız, E. camaldulensis esansiyel yağının mükemmel bir antipsödomonal kaynağı oluşturduğunu ve ayrıca Pseudomonas türlerinin biyofilm oluşumunu inhibe ettiğini gösterdi. Antibakteriyel ve antibiyofilm potansiyeline dayalı olarak, E. camaldulensis esansiyel yağı, potansiyel bir antibakteriyel ajan kaynağı olarak ümit vaat etmektedir. Sonuç olarak, E. camaldulensis uçucu yağının, patojen mikroorganizmalara karşı doğal bir antibakteriyel ajan olma potansiyeline sahip olduğu tespit edilmiştir.

Anahtar Kelimeler

• antibakteriyel aktivite
• antibiyofilm aktivite
• uçucu yağ
• okaliptüs

Kaynakça

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