MALAHİT YEŞİLİNİN GÖKKUŞAĞI ALABALIĞINDA SİTOKROM P450 VE ANTİOKSİDAN ENZİM AKTİVİTELERİ ÜZERİNE İN VİVO MODÜLATÖR ETKİSİ

Yıl 2025, Cilt: 3 Sayı: 1, 10 - 18, 30.06.2025

Azra Bozcaarmutlu Büken , Volkan Yıldırım , Canan Sapmaz , Ahmet Günbür , Hakan Türker

https://doi.org/10.62425/tjau.1656086

Öz

Malahit yeşili, tekstil ve kağıt endüstrisinde boya olarak ve balık çiftliklerinde ektoparazitlere, bakteriyel ve fungal enfeksiyonlara karşı kullanılan mutajenik ve karsinojenik bir kimyasaldır. Sucul organizmalar malahit yeşiline maruz kalma riski altındadır. Bu çalışmada amacımız gökkuşağı alabalığında (Oncorhynchus mykiss) iki farklı konsantrasyonda malahit yeşilinin sitokrom P450 ve antioksidan sistem üzerine etkisini belirlemektir. Bu amaçla, 24 balık üç gruba rastgele ayrılmıştır: kontrol, 0,1 mg/L malahit yeşili ile muamele edilen grup ve 0,5 mg/L malahit yeşili ile muamele edilen grup. Oldukça yüksek 7-etoksiresorufin O-deetilaz (EROD) aktivitesi balıklarda 0,5 mg/L malahit yeşili ile muamele edilen grupta ölçülmüştür. 7-pentoksiresorufin O-depentilaz (PROD) aktivitesi artan malahit yeşili dozu ile artmıştır. Katalaz (CAT) ve glutatyon redüktaz aktiviteleri 0,1 mg/L malahit yeşili ile muamele edilen grupta kontrol grubundan yüksektir. Eritromisin N-demetilaz (ERND) ve glutatyon S-transferaz (GST) aktiviteleri her iki muamele grubunda malahit yeşilinden önemli ölçüde etkilenmemiştir. Bu çalışmanın sonuçları malahit yeşilinin gökkuşağı alabalığında EROD, PROD, CAT ve GR aktiviteleri üzerinde modülatör bir etkiye sahip olduğunu açıkça göstermektedir.

Anahtar Kelimeler

Antioksidan enzim aktiviteleri , sitokrom P450 , gökkuşağı alabalığı (Oncorhynchus mykiss) , ksenobiyotik metabolize eden enzimler , malahit yeşili

Proje Numarası

project no: BAP –2019.03.03.1406

In VIVO MODULATORY EFFECT of MALACHITE GREEN on CYTOCHROME P450 AND ANTIOXIDANT ENZYME ACTIVITIES in RAINBOW TROUT LIVER

Öz

Malachite green is a mutagenic and carcinogenic chemical used as a dye in textile and paper industry and in fish farms against ectoparasites, bacterial and fungal infections. Aquatic organisms are at risk of malachite green exposure. In this study, our aim was to determine the effect of two different doses of malachite green on cytochrome P450 system and antioxidant system in rainbow trout (Oncorhynchus mykiss). For this purpose, 24 fish were randomly divided into three groups: the control, the 0.1 mg/L malachite green treatment group and the 0.5 mg/L malachite green treatment group. Highly elevated 7-ethoxyresorufin-O-deethylase activities (EROD) were measured in fish treated with 0.5 mg/L malachite green. 7-pentoxyresorufin O-depentylase (PROD) activities increased with increasing doses of malachite green. Catalase (CAT) and glutathione reductase (GR) activities in the 0.1 mg/L malachite green administration group were higher than those in the control group. Erythromycin N-demethylase (ERND) and glutathione S-transferase (GST) activities were not significantly affected by malachite green treatment in either treatment group. The results of this study clearly demonstrate that malachite green has a modulatory effect on EROD, PROD, CAT, and GR activities in rainbow trout.

Anahtar Kelimeler

Antioxidant enzyme activities , Cytochrome P450 , Malachite green , Rainbow trout (Oncorhynchus mykiss) , Xenobiotic metabolizing enzymes

Etik Beyan

Hayvan temelli çalışmalar için Bolu Abant İzzet Baysal Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu'ndan etik izin alınmıştır (İşlem numarası: 2018/21).

Destekleyen Kurum

Bu çalışma kısmen Bolu Abant Izzet Baysal Üniversitesi tarafından desteklenmiştir [project no: BAP –2019.03.03.1406].

Proje Numarası

project no: BAP –2019.03.03.1406

Teşekkür

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