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Marine Derived Chitin as a Promising Bio- Stimulant for Sustainable Agriculture

Yıl 2025, Cilt: 3 Sayı: 1, 19 - 28, 30.06.2025
https://doi.org/10.62425/tjau.1709307

Öz

Chitin and chitosan are natural compounds that are biodegradable and nontoxic, have garnered significant attention for their positive impact on crop yield and agro-environmental sustainability. As sustainable agriculture becomes more imperative, biocontrol using natural compounds such as chitin, a carbohydrate chain polymer, and its derivatives, is a promising strategy. Chitin and its derivatives induce or enhance natural defensive mechanisms in plants. They are recognized as plant growth regulators, growth stimulants, and elicitors for the production of secondary metabolites. They have beneficial effects as fertilizers, soil conditioning agents, plant disease control agents, antitranspirants, ripening retardants, and seed and fruit coatings. Chitinous materials (chitin and its derivatives) are obtained from renewable sources, mainly shellfish waste, having a great potential for the development of bioproducts as alternatives to synthetic agrochemicals. Recent studies have provided evidence that the use of these biopolymers can help control postharvest diseases, increase the content of nutrients available to plants, and elicit positive metabolic changes that lead to higher plant resistance against pathogens. Hence, the present study was aimed to collect crustaceans from coastal region and chitin extraction. The extracted chitin is fortified with soil mixture as manure and subjected to detect the growth of plants such as okra and tomato. The formulation and application of Chitin manure to soil and Analysis of Plant Growth Parameters at different time intervals is recorded and the data interpretation carried out.

Etik Beyan

This research article involves no studies with human or animal subjects and does not present any original data; therefore, no ethical approval was required.

Teşekkür

We sincerely thank you for the opportunity to submit our review article to your esteemed journal. We deeply appreciate your time and consideration and respectfully request that the manuscript be kindly processed for publication.

Kaynakça

  • Abdou, E. S., Nagy, K. S., & Elsabee, M. Z. (2008). Extraction and characterization of chitin and chitosan from local sources. Bioresource Technology, 99(5), 1359-1367.
  • Ait Barka, E., Eullaffroy, P., Clément, C., & Vernet, G. (2004). Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Reports, 22, 608-614.
  • Aklog, Y., F., Egusa, M., Kaminaka, H., Izawa, H., Morimoto, M., Saimoto, H., & Ifuku, S., (2016). Protein/ CaCO3/ Chitin Nanofiber Complex Prepared from Crab Shells by Simple Mechanical Treatment and Its Effect on Plant Growth. International Journal of Molecular Sciences, 17, 1600.
  • Aranaz, I., Mengibar, M., Harris, R., Paños, I., Miralles, B., Acosta, N., Galed, G., & Heras, Á. (2009). Functional characterization of chitin and chitosan. Current Chemical Biology, 3(2), 203-230.
  • Bautista-Baños, S., Hernandez-Lauzardo, A. N., Velazquez-Del Valle, M. G., Hernández-López, M., Barka, E. A., Bosquez- Molina, E., & Wilson, C. L. (2006). Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Protection, 25(2), 108-118.
  • Bhatnagar, A., & Sillanpaa, M. (2009). Applications of chitin- and chitosan-derivatives for the detoxification of water and wastewater-a short review. Advances in Colloid and Interface Science, 152(1-2), 26-38. Brown, P., & Saa, S. (2015). Biostimulants in agriculture. Frontiers in Plant Science, 6, 671.
  • Casadidio, C., Peregrina, D. V., Gigliobianco, M. R., Deng, S., Censi, R., & Di Martino, P. (2019). Chitin and chitosans: Characteristics, eco-friendly processes, and applications in cosmetic science. Marine Drugs, 17(6), 369.
  • Devi, R., & Dhamodharan, R. (2018). Pretreatment in hot glycerol for facile and green separation of chitin from prawn shell waste. ACS Sustainable Chemistry & Engineering, 6(1), 846-853.
  • Duan, B., Huang, Y., Lu, A., & Zhang, L. (2018). Recent advances in chitin based materials constructed via physical methods. Progress in Polymer Science, 82, 1-33.
  • El Hadrami, A., Adam, L. R., El Hadrami, I., & Daayf, F. (2010). Chitosan in plant protection. Marine Drugs, 8(4), 968-987. Gadgey, K., & Bahekar, A. (2017). Studies On Extraction Methods of Chitin from CRAB Shell and Investigation of Its Mechanical Properties. International Journal of Mechanical Engineering and Technology, (2), 220 - 231.
  • Hadwiger, L. A. (2013). Multiple effects of chitosan on plant systems: Solid science or hype. Plant Science, 208, 42-49.
  • Hajji, S., Ghorbel-Bellaaj, O., Younes, I., Jellouli, K., & Nasri, M. (2015). Chitin extraction from crab shells by Bacillus bacteria. Biological activities of fermented crab supernatants. International Journal of Biological Macromolecules, 79, 167–173.
  • Huang, S., Zheng, X., Luo, L., Ni, Y., Yao, L., & Ni, W. (2021). Biostimulants in bioconversion compost of organic waste: A novel booster in sustainable agriculture. Journal of Cleaner Production, 319, 128704.
  • Ifuku, S., Nogi, M., Abe, K., Yoshioka, M., Morimoto, M., Saimoto, H., & Yano, H. (2009). Preparation of chitin nanofibers with a uniform width as α-chitin from crab shells. Biomacromolecules, 10(6), 1584-1588.
  • Kasaai, M. R. (2010). Determination of the degree of N-acetylation for chitin and chitosan by various NMR spectroscopy techniques: A review. Carbohydrate Polymers, 79(4), 801-810.
  • Kaur, S., & Dhillon, G. S. (2015). Recent trends in biological extraction of chitin from marine shell wastes: A review. Critical Reviews in Biotechnology, 35(1), 44-61.
  • Kaya, M., Baran, T., & Karaarslan, M. (2015). A new method for fast chitin extraction from shells of crab, crayfish and shrimp. Natural Product Research, 29(15), 1477-1480.
  • Kumar, M. N. R. (2000). A review of chitin and chitosan applications. Reactive and Functional Polymers, 46(1), 1-27.
  • Li, K., Xing, R., Liu, S., & Li, P. (2020). Chitin and chitosan fragments responsible for plant elicitor and growth stimulator. Journal of Agricultural and Food Chemistry, 68(44), 12203-12211.
  • Mohan, K., Muralisankar, T., Jayakumar, R., & Rajeevgandhi, C. (2021). A study on structural comparisons of α-chitin extracted from marine crustacean shell waste. Carbohydrate Polymer Technologies and Applications, 2, 100037.
  • Muzzarelli, R. A. (2009). Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 76(2), 167-182.
  • Pandharipande, S., L., & Prakash, H. (2019). Bhagat Associate Professor, B. Tech. 8th Semester Department of Chemical Engineering, Laxminarayan Institute of Technology, Rashtrasant Tukdoji Maharaj Nagpur University, Nagpur City, India.
  • Pichyangkura, R., & Chadchawan, S. (2015). Biostimulant activity of chitosan in horticulture. Scientia Horticulturae, 196, 49–65.
  • Puglia, D., Pezzolla, D., Gigliotti, G., Torre, L., Bartucca, M.L., & Del Buono, D. (2021). The Opportunity of Valorizing Agricultural Waste, Through Its Conversion into Bio stimulants, Biofertilizers, and Biopolymers. Sustainability, 13(5), 2710.
  • Rkhaila, A., Chtouki, T., Erguig, H., Haloui, N., & Ounine, K. (2021). Chemical Proprieties of Biopolymers (Chitin/Chitosan) and Their Synergic Effects with Endophytic Bacillus Species: Unlimited Applications in Agriculture. Molecules, 26 (4), 1117.
  • Rouphael, Y., & Colla, G. (2020a). Biostimulants in agriculture. Frontiers in Plant Science, 11, 40.
  • Rouphael, Y., & Colla, G. (2020b). Toward a Sustainable Agriculture through Plant Bio stimulants: From Experimental Data to Practical Applications. Agronomy, 10(10), 1461.
  • Saharan, B. S., & Nehra, V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sci Med Res, 21(1), 30.
  • Shahrajabian, M. H., Chaski, C., Polyzos, N., Tzortzakis, N., & Petropoulos, S. A. (2021). Sustainable agriculture systems in vegetable production using chitin and chitosan as plant biostimulants. Biomolecules, 11(6), 819. Shaikh, J. R., & Patil, M. K. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608.
  • Sharp, R. E., LeNoble, M. E., & Else, M. A. (2000). Regulation of growth and development by ABA: a comparison of responses of maize and tomato to water stress. Journal of Experimental Botany, 51(350), 289-299. Sirajudheen, P., Poovathumkuzhi, N. C., Vigneshwaran, S., Chelaveettil, B. M., & Meenakshi, S. (2021). Applications of chitin and chitosan based biomaterials for the adsorptive removal of textile dyes from water—A comprehensive review. Carbohydrate Polymers, 273, 118604.
  • Sreekumar, S., Gopinath, S., & Meenakshisundaram, M. (2018). Bio-stimulant effect of chitosan on seed germination and plant growth in Brassica oleracea. International Journal of Biological Macromolecules, 112, 1040–1044.
  • Wang, S. L., & Chio, S. H. (1998). Deproteinization of shrimp and crab shell with the protease of Pseudomonas aeruginosa K-187. Enzyme and Microbial Technology, 22(7), 629-633.
  • Xu, Y., Gallert, C., & Winter, J. (2008). Chitin purification from shrimp wastes by microbial deproteination and decalcification. Applied Microbiology and Biotechnology, 79, 687-697.
  • Younes, I., & Rinaudo, M. (2015). Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine drugs, 13(3), 1133-1174.
  • Zhang, J., Feng, M., Lu, X., Shi, C., Li, X., Xin, J., Yue, G., & Zhang, S. (2018). Base-free preparation of low molecular weight chitin from crab shell. Carbohydrate Polymers, 190, 148-155.

Marine Derived Chitin as a Promising Bio- Stimulant for Sustainable Agriculture

Yıl 2025, Cilt: 3 Sayı: 1, 19 - 28, 30.06.2025
https://doi.org/10.62425/tjau.1709307

Öz

Chitin and chitosan are natural compounds that are biodegradable and nontoxic, have garnered significant attention for their positive impact on crop yield and agro-environmental sustainability. As sustainable agriculture becomes more imperative, biocontrol using natural compounds such as chitin, a carbohydrate chain polymer, and its derivatives, is a promising strategy. Chitin and its derivatives induce or enhance natural defensive mechanisms in plants. They are recognized as plant growth regulators, growth stimulants, and elicitors for the production of secondary metabolites. They have beneficial effects as fertilizers, soil conditioning agents, plant disease control agents, antitranspirants, ripening retardants, and seed and fruit coatings. Chitinous materials (chitin and its derivatives) are obtained from renewable sources, mainly shellfish waste, having a great potential for the development of bioproducts as alternatives to synthetic agrochemicals. Recent studies have provided evidence that the use of these biopolymers can help control postharvest diseases, increase the content of nutrients available to plants, and elicit positive metabolic changes that lead to higher plant resistance against pathogens. Hence, the present study was aimed to collect crustaceans from coastal region and chitin extraction. The extracted chitin is fortified with soil mixture as manure and subjected to detect the growth of plants such as okra and tomato. The formulation and application of Chitin manure to soil and Analysis of Plant Growth Parameters at different time intervals is recorded and the data interpretation carried out

Kaynakça

  • Abdou, E. S., Nagy, K. S., & Elsabee, M. Z. (2008). Extraction and characterization of chitin and chitosan from local sources. Bioresource Technology, 99(5), 1359-1367.
  • Ait Barka, E., Eullaffroy, P., Clément, C., & Vernet, G. (2004). Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Reports, 22, 608-614.
  • Aklog, Y., F., Egusa, M., Kaminaka, H., Izawa, H., Morimoto, M., Saimoto, H., & Ifuku, S., (2016). Protein/ CaCO3/ Chitin Nanofiber Complex Prepared from Crab Shells by Simple Mechanical Treatment and Its Effect on Plant Growth. International Journal of Molecular Sciences, 17, 1600.
  • Aranaz, I., Mengibar, M., Harris, R., Paños, I., Miralles, B., Acosta, N., Galed, G., & Heras, Á. (2009). Functional characterization of chitin and chitosan. Current Chemical Biology, 3(2), 203-230.
  • Bautista-Baños, S., Hernandez-Lauzardo, A. N., Velazquez-Del Valle, M. G., Hernández-López, M., Barka, E. A., Bosquez- Molina, E., & Wilson, C. L. (2006). Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Protection, 25(2), 108-118.
  • Bhatnagar, A., & Sillanpaa, M. (2009). Applications of chitin- and chitosan-derivatives for the detoxification of water and wastewater-a short review. Advances in Colloid and Interface Science, 152(1-2), 26-38. Brown, P., & Saa, S. (2015). Biostimulants in agriculture. Frontiers in Plant Science, 6, 671.
  • Casadidio, C., Peregrina, D. V., Gigliobianco, M. R., Deng, S., Censi, R., & Di Martino, P. (2019). Chitin and chitosans: Characteristics, eco-friendly processes, and applications in cosmetic science. Marine Drugs, 17(6), 369.
  • Devi, R., & Dhamodharan, R. (2018). Pretreatment in hot glycerol for facile and green separation of chitin from prawn shell waste. ACS Sustainable Chemistry & Engineering, 6(1), 846-853.
  • Duan, B., Huang, Y., Lu, A., & Zhang, L. (2018). Recent advances in chitin based materials constructed via physical methods. Progress in Polymer Science, 82, 1-33.
  • El Hadrami, A., Adam, L. R., El Hadrami, I., & Daayf, F. (2010). Chitosan in plant protection. Marine Drugs, 8(4), 968-987. Gadgey, K., & Bahekar, A. (2017). Studies On Extraction Methods of Chitin from CRAB Shell and Investigation of Its Mechanical Properties. International Journal of Mechanical Engineering and Technology, (2), 220 - 231.
  • Hadwiger, L. A. (2013). Multiple effects of chitosan on plant systems: Solid science or hype. Plant Science, 208, 42-49.
  • Hajji, S., Ghorbel-Bellaaj, O., Younes, I., Jellouli, K., & Nasri, M. (2015). Chitin extraction from crab shells by Bacillus bacteria. Biological activities of fermented crab supernatants. International Journal of Biological Macromolecules, 79, 167–173.
  • Huang, S., Zheng, X., Luo, L., Ni, Y., Yao, L., & Ni, W. (2021). Biostimulants in bioconversion compost of organic waste: A novel booster in sustainable agriculture. Journal of Cleaner Production, 319, 128704.
  • Ifuku, S., Nogi, M., Abe, K., Yoshioka, M., Morimoto, M., Saimoto, H., & Yano, H. (2009). Preparation of chitin nanofibers with a uniform width as α-chitin from crab shells. Biomacromolecules, 10(6), 1584-1588.
  • Kasaai, M. R. (2010). Determination of the degree of N-acetylation for chitin and chitosan by various NMR spectroscopy techniques: A review. Carbohydrate Polymers, 79(4), 801-810.
  • Kaur, S., & Dhillon, G. S. (2015). Recent trends in biological extraction of chitin from marine shell wastes: A review. Critical Reviews in Biotechnology, 35(1), 44-61.
  • Kaya, M., Baran, T., & Karaarslan, M. (2015). A new method for fast chitin extraction from shells of crab, crayfish and shrimp. Natural Product Research, 29(15), 1477-1480.
  • Kumar, M. N. R. (2000). A review of chitin and chitosan applications. Reactive and Functional Polymers, 46(1), 1-27.
  • Li, K., Xing, R., Liu, S., & Li, P. (2020). Chitin and chitosan fragments responsible for plant elicitor and growth stimulator. Journal of Agricultural and Food Chemistry, 68(44), 12203-12211.
  • Mohan, K., Muralisankar, T., Jayakumar, R., & Rajeevgandhi, C. (2021). A study on structural comparisons of α-chitin extracted from marine crustacean shell waste. Carbohydrate Polymer Technologies and Applications, 2, 100037.
  • Muzzarelli, R. A. (2009). Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 76(2), 167-182.
  • Pandharipande, S., L., & Prakash, H. (2019). Bhagat Associate Professor, B. Tech. 8th Semester Department of Chemical Engineering, Laxminarayan Institute of Technology, Rashtrasant Tukdoji Maharaj Nagpur University, Nagpur City, India.
  • Pichyangkura, R., & Chadchawan, S. (2015). Biostimulant activity of chitosan in horticulture. Scientia Horticulturae, 196, 49–65.
  • Puglia, D., Pezzolla, D., Gigliotti, G., Torre, L., Bartucca, M.L., & Del Buono, D. (2021). The Opportunity of Valorizing Agricultural Waste, Through Its Conversion into Bio stimulants, Biofertilizers, and Biopolymers. Sustainability, 13(5), 2710.
  • Rkhaila, A., Chtouki, T., Erguig, H., Haloui, N., & Ounine, K. (2021). Chemical Proprieties of Biopolymers (Chitin/Chitosan) and Their Synergic Effects with Endophytic Bacillus Species: Unlimited Applications in Agriculture. Molecules, 26 (4), 1117.
  • Rouphael, Y., & Colla, G. (2020a). Biostimulants in agriculture. Frontiers in Plant Science, 11, 40.
  • Rouphael, Y., & Colla, G. (2020b). Toward a Sustainable Agriculture through Plant Bio stimulants: From Experimental Data to Practical Applications. Agronomy, 10(10), 1461.
  • Saharan, B. S., & Nehra, V. (2011). Plant growth promoting rhizobacteria: a critical review. Life Sci Med Res, 21(1), 30.
  • Shahrajabian, M. H., Chaski, C., Polyzos, N., Tzortzakis, N., & Petropoulos, S. A. (2021). Sustainable agriculture systems in vegetable production using chitin and chitosan as plant biostimulants. Biomolecules, 11(6), 819. Shaikh, J. R., & Patil, M. K. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603–608.
  • Sharp, R. E., LeNoble, M. E., & Else, M. A. (2000). Regulation of growth and development by ABA: a comparison of responses of maize and tomato to water stress. Journal of Experimental Botany, 51(350), 289-299. Sirajudheen, P., Poovathumkuzhi, N. C., Vigneshwaran, S., Chelaveettil, B. M., & Meenakshi, S. (2021). Applications of chitin and chitosan based biomaterials for the adsorptive removal of textile dyes from water—A comprehensive review. Carbohydrate Polymers, 273, 118604.
  • Sreekumar, S., Gopinath, S., & Meenakshisundaram, M. (2018). Bio-stimulant effect of chitosan on seed germination and plant growth in Brassica oleracea. International Journal of Biological Macromolecules, 112, 1040–1044.
  • Wang, S. L., & Chio, S. H. (1998). Deproteinization of shrimp and crab shell with the protease of Pseudomonas aeruginosa K-187. Enzyme and Microbial Technology, 22(7), 629-633.
  • Xu, Y., Gallert, C., & Winter, J. (2008). Chitin purification from shrimp wastes by microbial deproteination and decalcification. Applied Microbiology and Biotechnology, 79, 687-697.
  • Younes, I., & Rinaudo, M. (2015). Chitin and chitosan preparation from marine sources. Structure, properties and applications. Marine drugs, 13(3), 1133-1174.
  • Zhang, J., Feng, M., Lu, X., Shi, C., Li, X., Xin, J., Yue, G., & Zhang, S. (2018). Base-free preparation of low molecular weight chitin from crab shell. Carbohydrate Polymers, 190, 148-155.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Balık Biyolojisi, Sucul Kültür
Bölüm Araştırma Makalesi
Yazarlar

Govindaraju Rajalakshmi 0000-0002-0851-5055

Siva Balan 0009-0004-3440-5660

Raghul K Bu kişi benim 0009-0007-5013-8197

G. Brindha Sankaran 0009-0000-9413-0567

Gönderilme Tarihi 31 Mayıs 2025
Kabul Tarihi 27 Haziran 2025
Erken Görünüm Tarihi 30 Haziran 2025
Yayımlanma Tarihi 30 Haziran 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 3 Sayı: 1

Kaynak Göster

APA Rajalakshmi, G., Balan, S., K, R., Sankaran, G. B. (2025). Marine Derived Chitin as a Promising Bio- Stimulant for Sustainable Agriculture. The Trout Journal of Atatürk University, 3(1), 19-28. https://doi.org/10.62425/tjau.1709307

The Trout Journal of Atatürk University (Atatürk Üniversitesi Alabalık Dergisi)

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