LANTANA CAMARA L.: A NATURAL TREASURE TROVE OF ANTIOXIDANTS AND ANTI-INFLAMMATORY AGENTS

Yıl 2025, Cilt: 3 Sayı: 1, 1 - 9, 30.06.2025

Antony Godson S G

https://izlik.org/JA26FB33KH

Öz

Lantana camara L. leaves were extracted using solvents of differing polarity—petroleum ether, acetone, and chloroform—to investigate their phytochemical composition, functional groups, and antioxidant potential. Preliminary phytochemical screening revealed a rich presence of bioactive metabolites such as flavonoids, phenolics, alkaloids, tannins, terpenoids, glycosides, and steroids predominantly in acetone and chloroform extracts, while petroleum ether showed limited polarity-associated compounds. Quantitative analysis demonstrated significantly higher total phenolic (54.86 mg GAE/g) and flavonoid content (39.44 mg QE/g) in acetone extracts compared to petroleum ether (8.72 mg GAE/g and 5.31 mg QE/g) and chloroform (28.17 mg GAE/g and 19.25 mg QE/g). Antioxidant assays corroborated these findings: acetone extract exhibited the strongest DPPH radical scavenging activity (IC₅₀ = 41.25 μg/mL), highest ferric reducing antioxidant power (429.55 μmol Trolox equivalents/g), and ABTS inhibition (88.36%). Fourier Transform Infrared (FTIR) spectroscopy confirmed the presence of key functional groups, including hydroxyl, carbonyl, and aromatic moieties, varying with solvent polarity. These results highlight acetone as the most effective solvent for extracting antioxidant-rich phytochemicals from L. camara leaves, emphasizing the plant’s potential as a natural source of therapeutic antioxidants for pharmaceutical and nutraceutical applications.

Anahtar Kelimeler

Lantana camara , Bioactive compounds , Antioxidant activity , FTIR

LANTANA CAMARA L.: ANTİOKSİDANLAR VE ANTİ-İNFLAMATUAR AJANLARIN DOĞAL HAZİNESİ

https://izlik.org/JA26FB33KH

Öz

Lantana camara L. yaprakları, fitokimyasal bileşimlerini, fonksiyonel gruplarını ve antioksidan potansiyellerini araştırmak için farklı polariteye sahip çözücüler (petrol eteri, aseton ve kloroform) kullanılarak ekstrakt edildi. Ön fitokimyasal tarama, aseton ve kloroform özütlerinde baskın olarak flavonoidler, fenolikler, alkaloidler, tanenler, terpenoidler, glikozitler ve steroidler gibi biyoaktif metabolitlerin zengin bir varlığını ortaya koyarken, petrol eteri sınırlı polariteyle ilişkili bileşikler gösterdi. Kantitatif analiz, aseton özütlerinde petrol eteri (8,72 mg GAE/g ve 5,31 mg QE/g) ve kloroform (28,17 mg GAE/g ve 19,25 mg QE/g) ile karşılaştırıldığında önemli ölçüde daha yüksek toplam fenolik (54,86 mg GAE/g) ve flavonoid içeriği (39,44 mg QE/g) gösterdi. Antioksidan analizleri bu bulguları doğruladı: aseton özütü en güçlü DPPH radikal temizleme aktivitesini (IC₅₀ = 41,25 μg/mL), en yüksek ferrik indirgeyici antioksidan gücünü (429,55 μmol Trolox eşdeğeri/g) ve ABTS inhibisyonunu (%88,36) gösterdi. Fourier Dönüşümlü Kızılötesi (FTIR) spektroskopisi, çözücü polaritesine göre değişen hidroksil, karbonil ve aromatik gruplar dahil olmak üzere temel fonksiyonel grupların varlığını doğruladı. Bu sonuçlar, asetonu L. camara yapraklarından antioksidan açısından zengin fitokimyasalları çıkarmak için en etkili çözücü olarak vurgulayarak, bitkinin farmasötik ve nutrasötik uygulamalar için doğal bir terapötik antioksidan kaynağı olarak potansiyelini vurguladı.

Anahtar Kelimeler

Lantana camara , Biyoaktif bileşikler , Antioksidan aktivite , FTIR

Kaynakça

• Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76.
• Bhowmik, D., Kumar, K. P. S., Paswan, S., & Srivastava, S. (2013). Traditional medicinal plants and their role in cancer treatment and prevention. Journal of Pharmacognosy and Phytochemistry, 1(1), 1–11. https://www.phytojournal.com/vol1Issue1/ Issue_july_2012/ 1.1.pdf
• Bhuvaneshwari, V., Krishnan, V., & Ilango, K. (2017). Evaluation of phytochemical constituents and antioxidant activity of Lantana camara L. leaves. Asian Journal of Pharmaceutical and Clinical Research, 10(3), 229–232. https://doi.org/ 10.22159 /ajpcr.2017.v10i3.15892
• Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25–30.
• Chandra, S., Khan, S., Avula, B., Lata, H., Yang, M. H., Elsohly, M. A., & Khan, I. A. (2016). Assessment of total phenolic and flavonoid content, antioxidant properties, and yield of aerial parts of Micropropagated Lantana camara L.. Pharmacognosy Magazine, 12(46), S425–S430. https://doi.org/10.4103/0973-1296.185770
• Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3), 296–302. https://doi.org/10.1016/j.jfda.2013.11.001
• Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics, 11(1), 1–42. https://doi.org/10.2307/3001478
• Ghosh, M., Manivannan, R., & Sil, P. C. (2015). Investigation of the therapeutic potential of Withania somnifera root extract in the prevention of bromobenzene-induced hepatic and renal disorders. Toxicology Reports, 2, 648–658. https://doi.org/10.1016/ j.toxrep.2015.04.007
• Harborne, J. B. (1998). Phytochemical methods: A guide to modern techniques of plant analysis (3rd ed.). Chapman & Hall.
• Hossain, M. A., Shah, M. D., & Gnanaraj, C. (2016). In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from leaves of tropical medicinal plant Tetrastigma hemsleyanum. Asian Pacific Journal of Tropical Medicine, 6(9), 689–695. https://doi.org/10.1016/S1995-7645(13)60121-2
• Hostettmann, K., & Marston, A. (1995). Saponins. Cambridge University Press.
• Jassim, S. A. A., & Naji, M. A. (2003). Novel antiviral agents: A medicinal plant perspective. Journal of Applied Microbiology, 95(3), 412–427. https://doi.org/ 10.1046/j.1365-2672.2003. 02026.x
• Jiang, H., Zhan, W. Q., Liu, X. D., & Jiang, S. X. (2011). Antioxidant activities of extract and fractions from Polygonum orientale L. International Journal of Molecular Sciences, 12(9), 6123–6137. https://doi.org/10.3390/ijms12096123
• Kaur, G. J., & Arora, D. S. (2009). Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complementary and Alternative Medicine, 9, 30. https://doi.org/10.1186/1472-6882-9-30
• Kumar, R., Singh, P., & Kumar, N. (2021). Fourier-transform infrared (FTIR) spectroscopy analysis of plant extracts for assessment of quality and authenticity. Current Research in Green and Sustainable Chemistry, 4, 100072. https://doi.org/10.1016/j.crgsc.2021.100072
• Kumar, S., Kumar, D., Deshmukh, R. R., Waghmode, M. S., & Naik, V. G. (2017). Phytochemical screening and FTIRspectroscopic studies of Mentha arvensis L. leaves. International Journal of Herbal Medicine, 5(6), 41–44.
• Mason, R., Smith, L., & Brown, K. (2019). Advances in plant-based antioxidants: Extraction, characterization, and applications. Journal of Agricultural and Food Chemistry, 67(14), 3921–3934. https://doi.org/10.1021/acs.jafc.9b00908
• McHugh, M. L., & Krieger, S. (2005). Tips for understanding and using confidence intervals. Practical Assessment, Research, and Evaluation, 10(14), 1–8. https://doi.org/10.7275/dkx0-3b09
• Mendoza-Castillo, D. I., Reynel-Ávila, H. E., Bonilla-Petriciolet, A., & Rivera-Utrilla, J. (2017). Adsorption of dyes from aqueous solution onto low-cost biosorbents. In M. N. Vara Prasad & M. Shih (Eds.), Environmental materials and waste: Resource recovery and pollution prevention (pp. 493–520). Academic Press. https://doi.org/10.1016/B978-0-12-803837-6.00026-2
• Nita, M., Grzybowski, A., & Pawlak, A. (2020). The role of oxidative stress in ocular diseases: A review. Oxidative Medicine and Cellular Longevity, 2020, 4263680. https://doi.org/10.1155/2020/4263680
• Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5, e47. https://doi.org/10.1017/jns.2016.41
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3PubMed+4The University of Brighton+4ScienceDirect+4
• Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4), 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
• Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Latha, L. Y. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1–10. G Singh, A., Kumar, R., & Patel, S. (2018). FTIR analysis and antioxidant evaluation of plant extracts: A comparative study. Phytochemistry Letters, 24, 27–33. https://doi.org/10.1016/j.phytol.2017.10.006
• Singh, P., Gupta, V., & Sharma, N. (2013). Phenolic compounds and antioxidant activity of medicinal plants: Extraction optimization and characterization. Industrial Crops and Products, 145, 111968. https://doi.org/10.1016/j.indcrop.2020.111968.
• Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152–178. https://doi.org/10.1016/S0076-6879(99)99017-1Semantic Scholar+4ResearchGate+4ScienceDirect+4