Sigara ile İndüklenen Böbrek Hasarına Vitamin E ve N-Nitro L-Arginin Metil Esterin (L-NAME) Koruyucu Etkisi

Öz

Sigara, tütün bitkisinin yapraklarından elde edilen ve birçok insan tarafından rahatlatıcı etkisi olduğuna inanılan bir maddedir. Sigara bileşenlerinin, oksidatif stres maruziyeti nedeniyle böbrek fonksiyonu üzerinde olumsuz etkileri olduğu bilinmektedir. E vitamini organizmayı çeşitli mekanizmalarla koruyan ve hücresel boyutta hasarı önleyen önemli bir antioksidan olarak bilinir. L-NAME nitrik oksit (NO) sentaz enzimini inhibe ederek NO oluşumunu azaltır ve reaktif türlerin neden olduğu oksidatif hasarı azaltmaya katkıda bulunur. Bu çalışmada, sigara maruziyetinin sıçan böbrekleri üzerindeki etkisini ve E vitamini ile L-NAME'nin koruyucu etkilerini araştırmayı amaçladık. Bu amaçla, kırk beş erkek albino sıçan beş gruba ayrıldı: kontrol, sigara, sigara + E vitamini, sigara + L-NAME, sigara + E vitamini + L-NAME. Sıçanlar, özel kafeslerde inhalasyon ile sigara dumanına maruz bırakılmış ve 42 gün boyunca her gün tedavi gruplarına 200 mg/kg BW Vitamin E ve/veya 50 mg/kg BW L-NAME intraperitoneal olarak uygulanmıştır. 42. günün sonunda, sıçanların böbrek dokuları ve kanı biyokimyasal ve histolojik analiz için alındı. Sıçan serumunda kreatinin ve kan üre azotu (BUN) ile böbrek dokusu homojenatında malondialdehid (MDA), glutatyon (GSH) ve katalaz (CAT) ölçümleri yapıldı. Sigaraya maruz kalan sıçanlarda serumda kreatinin, BUN ve dokuda ise MDA düzeyleri anlamlı olarak artmışken, GSH düzeyleri ve CAT aktivitesi anlamlı olarak azaldı. Serumda kreatinin, BUN, dokuda ise MDA, GSH düzeyleri ve CAT aktivitesinin tedavi gruplarında normalize olduğu görüldü (p<0.05). Histolojik incelemeler, tedavi gruplarında iyileşme olduğunu gösterdi. Bu çalışmada sigara maruziyetinden kaynaklanan böbrek hasarının, E vitamini ve L-NAME kullanımı ile düzeltilebileceği görülmüştür.

Anahtar Kelimeler

• Sigara,Böbrek hasarı,L-NAME,vitamin E

Protective Effect of Vitamin E and N-Nitro L-Arginine Methyl Ester (L-NAME) on Cigarette Induced Kidney Damage

Abstract

The Cigarette is a material obtained from the leaves of a tobacco plant and believed to be comforting by many people. Cigarette components are known to have adverse effects on renal function because of oxidative stress exposure. Vitamin E is known to be an important antioxidant that protects the organism with various mechanisms and prevents damage at the cellular dimension. L-NAME inhibits nitric oxide (NO) synthase, reducing NO formation and contributing to the reduction of oxidative damage induced by reactive species. In this study, we aimed to investigate the effects of cigarette exposure on rat kidneys and protective effect of vitamin E and L-NAME. For this purpose, Forty five male albino rats were divided into five groups: control, cigarette, cigarette + vitamin E, cigarette + L-NAME, cigarette + vitamin E + L-NAME. The rats were exposed to cigarette smoke by inhalation in special cages and 200 mg/kg BW Vitamin E and/or 50 mg/kg BW L-NAME administered intraperitoneal for 42 days. These procedures were applied every day. At the end of the 42^nd day, the kidney tissues and blood of rats were taken for biochemical and histological analysis. Malondialdehyde (MDA), glutathione (GSH) and catalase (CAT) measurements were performed on the kidney tissue homogenate, creatinine and blood urea nitrogen (BUN) were measured in the rat serum. While serum creatinine, BUN and tissue MDA levels significantly increased, GSH levels and CAT activity decreased significantly in cigarette-exposed rats. Serum creatinine, BUN, MDA, GSH and CAT levels were normalized in the treatment groups (p<0.05). Histological examinations showed that there was improvement in the treatment groups. It has been observed that kidney damage resulting from cigarette-exposure can be eliminated by the use of vitamin E and L-NAME.

Keywords

• Cigarette,Kidney damage,L-NAME,vitamin E

Kaynakça

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