Exopolysaccharide Production by Root Nodule Bacteria of Wild Lathyrus cassius Boiss.

Yıl 2016, Cilt: 1 Sayı: 1, 1 - 7, 30.06.2016

Çiğdem Küçük , Cenap Cevheri

Öz

In this study, it was investigated that producing ability of extracellular polysaccharides (EPS) of the Rhizobium strains isolated from root nodules of wild Lathyrus cassius. The results provided that when isolates were grown in a chemically defined medium carbon and nitrogen sources itested in this study influced EPS synthesis. Maximum EPS production (1778 μg/ml and 1762.6 μg/ml, respectively) was obtained when the chemically defined medium was supplemented with mannitol and potassium nitrate, respectively which was accompanied a great increase in the production compared to the control. In all isolates, the maximum EPS production was 72 h, 150 rpm at mannitol and potassium nitrate containing medium.

Anahtar Kelimeler

Rhizobium sp., isolates, exopolysaccharide, carbon and nitrogen source

Yabani Lathyrus cassius Boiss. Kök Nodül Bakterileri Tarafından Ekzopolisakkarit Üretimi

Öz

Çalışmada, yabani Lathyrus cassius’ün kök nodüllerinden izole edilen Rhizobium sp. izolatlarının ekzopolisakkarit (EPS) üretimleri araştırılmıştır. İzolatlar belirli bir kimyasal ortamda geliştirildiklerinde, test edilen karbon ve azot kaynakları EPS üretimini etkilemiştir. Ortama sırasıyla mannitol ve potasyum nitrat eklendiğinde kontrolle karşılaştırıldığında EPS üretiminin (sırasıyla, 1778 µg/ml ve 1762.6 µg/ml) maksimum değerde olduğu belirlenmiştir. İzolatların tümünde maksimum EPS üretimi 150 rpm’de 72 saatte, mannitol ve potasyum nitrat içeren ortamda bulunmuştur

Anahtar Kelimeler

Rhizobium sp., izolatlar, ekzopolisakkarit, karbon ve azot kaynağı

Kaynakça

• Becker A, Pühler A. 1998. Production of polysaccharides. In: Spaink, H.P., Kondorosi, A., Hooykaas, P.J.J (eds). The Rhizobiaceae: molecular biology of model plant-associated bacteria. Kluwer Academic Publishers, Norwell.
• Bomfeti CA, Florentino AF, Guimaraes AP, Cardoso PG, Guerreiro MC, Moreira FMS. 2011. Exopolysaccharides produced by the symbiotic nitrogen fixing bacteria of leguminosae. Revista Brasileira de Ciencia do Solo, 35: 657-671.
• Breedveld MW, Zevenhevizen LPT, Conter-Cremes HCJ, Zehnder AJB. 1993. Influence of growth conditions on production of capsular and extracellular
• polysaccharides by Rhizobium leguminosarum. Ant Leeuw Microbiol, 64:1-8.
• Cunningham SD, Munns DN. 1984. The correlation between extracellular polysaccharide production and acid tolerance in Rhizobium. Soil Sci Soc Am J, 48:1273–1276.
• D’Haeze W, Glushka J, Rycke RD, Holsters M, Carlson RW. 2004. Structural characterization of extracellular polysaccharides of Azorhizobium caulinodans and importance for nodule initiation on Sesbania rostrata. Mol Microbiol, 52:485–500.
• Datta C, Basu PS. 1999a. Production of extracellular polysaccharides by a Rhizobium species from root nodules of Cajanus cajan. Acta Biotechnol, 19:59–68.
• Donot F, Fontana A, Baccou JC, Schorr-Galindo S. 2012. Microbial exopolysaccharides: Main examples of synthesis, excretion genetics and extraction. Carbohdrate Polymers, 87: 951-962.
• Downie JA. 2010. The roles of extracellular proteins, polysaccharides and signals in the interactions of rhizobia with legume roots. FEMS Microbiol Rev, 34:150-170.
• DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem, 28:350–356.
• Dudman WF. 1964. Growth and extracellular polysaccharide production by Rhizobium meliloti in defined medium. Journal of Bacteriol, 88: 640-645.
• Ghosh AC, Ghosh S, Basu PS. 2005. Production of extracellular polysaccharide by a Rhizobium species from root nodules of the leguminous tree Dalbergia lanceolaria. Eng Life Sci, 5:378–382.
• Hollingsworth R, Smith E, Ahmad MH. 1985. Chemical composition of extracellular polysaccharides of cowpea rhizobia. Arch Microbiol, 142: 18-20.
• Janczarek M, Rachwal K, Ciesla J, Ginalska G, Bieganowski A. 2015. Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties. Plant Soil, 388:211-227.
• Kaci Y, Heyraud A, Barakat M, Heulin T. 2005. Isolation and identification of an EPS producing Rhizobium strain from and soil (Algeria): Characterization of its EPS and the effect of inoculation on wheat rhizosphere soil structure. Research in Microbiol, 156: 522-531.
• Karr DB, Liang RT, Reuhs B, Emerich D. 2000. Altered exopolysaccharides of Bradyrhizobium japonicum mutants correlate with impaired soybean lectin binding, but not with effective nodule formation. Planta. 211: 218-226.
• Küçük Ç, Kıvanç M. 2009. Extracellular polysaccharide production by Rhizobium ciceri from Turkey. Annals of Microbiol, 59:141-144.
• Lloret J, Wulff BBH, Rubio JM, Downie JA, Bonialla IR, Rivilla R. 1998. Exopolysaccharide II production is regulated by salt in the halotolerant strain R.meliloti EFBl. Appl, Environ, Microbiol, 64:1024-1028.
• Oldroyd GE, Murray JD, Poole PS, Downie JA. 2011. The rules of engagement in the legume-Rhizobium symbiosis. Annu, Rev, Genet, 14: 119-144.
• Rinaudi L, Fujishige NA, Hirch AM, Banchio E, Zorreguieta A, Giordano W. 2006. Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation. Res Microbiol, 157: 867-875.
• Sayyed RZ, Jamadar DD, Patel PR. 2011. Production of Exopolysaccharide by Rhizobium sp. Indian J Microbiol, 51:294-300.
• Staudt AK, Wolfe LG, Shrout JD. 2012. Variations in exopolysaccharide production by Rhizobium tropici. Archives of Microbiol, 194:197-206.
• Upadhyay SK, Singh JS, Sinh DP. 2011. Exopolysaccharide producing plant growth promoting rhizobacteria under salinity condition. Pedosphere. 21: 214-222.
• Vargas-Garcia MC, Lopez MJ, Elorrieta MA, Suarez J, Moreno J. 2003. Properties of polysaccharide produced by Azotobacter vinelandii cultured on 4- hydroxybenzoic acid. J Appl Microbiol, 94:388–395.
• Vincent JM. 1970. A mannual for the pratical study of the root nodule bacteria. IBP. Handbook. No:15. Blackwell Scientific Publications. Oxford England.
• Zevenhuizen LPTM. 1986. Selective synthesis of polysaccharides by Rhizobium trifolii strain TA-1. FEMS Microbiol Lett, 35: 43-47.