Pyracantha coccinea’nın Metanol Ekstrelerinin Antimitotik ve Antimutajenik Etkilerinin Araştırılması

Yıl 2017, Cilt: 2 Sayı: 1, 110 - 116, 30.06.2017

Mehmet Cengiz Karaismailoğlu

Öz

Bu araştırmada, Pyracantha coccinea metanol ekstraktlarının Allium cepa somatik hücreleri üzerinde antimitotik ve antimutajenik etkileri değerlendirildi. Soğanlara ekstraktın 400, 800, 1600, and 3200 μg/mL dozları 6, 12 ve 24 saat için uygulandı. Saf su negatif kontrol olarak kullanıldı ve etilmetansülfonat bir pozitif kontrol olarak kullanıldı. Mitotik indeks çoğunlukla artan ekstrakt konsantrasyonlarıyla azaldı. Mitotik anormallikler düzensiz profaz, yapışık kromozom, geri kalmış kromozom ve c-mitoz olarak gözlendi. Ayrıca, mikroçekirdek sıklığı interfazda hesaplandı. Böylelikle P. coccinea metanol ekstraktlarının antimitotik ve antimutajenik potansiyeli çeşitli testlerle A. cepa kök ucu hücrelerinin kullanılmasıyla değerlendirildi ve belirlenen antimutajenik dozun kullanımı önerildi.

Anahtar Kelimeler

Pyracantha coccinea, Allium cepa, Metanol ekstresi, Antimitotik, Antimutajenik, Antimutajenik

Investigation of the Antimitotic and Antimutagenic Effects of Methanolic Extracts of Pyracantha coccinea

Öz

In this investigation, the antimitotic and antimutagenic effects of Pyracantha coccinea methanol extracts on somatic cells of Allium cepa was evaluated. The onion bulbs were applied to 400, 800, 1600, and 3200 μg /mL doses of the extract for 6, 12 and 24 h. Distilled water was utilized as a negative control and ethylmethanesulfonate was utilized as a positive control. The mitotic index mostly declined as increasing the extract concentrations. The mitotic abnormalities were observed as disturbed prophase, stickiness, laggards, chromatid bridges and c-mitosis. Also, micronucleus frequency was calculated in interphase. Thence, the antimitotic and antimutagenic potency of P. coccinea methanol extract with various tests were evaluated by using the root tip cells of A. cepa, and the use of a defined antimutagenic dose was proposed.

Anahtar Kelimeler

Pyracantha coccinea, Allium cepa, Methanolic extract, Antimitotic, Antimutagenic, Antimutagenic

Kaynakça

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