LPS ile Oluşturulan Sepsisli Sıçanlarda Metformin ile Erken Tedavi, Pankreatik Hasarı Azaltır

Yıl 2018, Cilt: 3 Sayı: 1, 194 - 199, 29.06.2018

Ezgi Kar İ. Özkan Alataş Varol Şahintürk Semih Öz

Öz

Bu çalışmada,
metforminin lipopolisakkarit (LPS) ile indüklenen sepsis sonrası pankreas
dokusu üzerindeki etkilerinin araştırılması amaçlanmıştır. Çalışmada 30 adet
Sprague Dawley cinsi erkek sıçan kullanılmıştır. Gruplar 5 adet ve her grupta 6
tane hayvan olacak şekilde; kontrol, sepsis, sepsis+1 saat önce metformin,
sepsis+1 saat sonra metformin ve sepsis+3 saat sonra metformin olarak
belirlenmiştir. LPS ve metformin dozları sırası ile 5 mg/kg ve 200 mg/kg olarak
belirlenmiş olup hayvanlara intraperitoneal olarak uygulanmıştır. LPS
enjeksiyonundan 24 saat sonra hayvanların kan örnekleri ve pankreas dokuları
cerrahi işlemlerle alınmıştır. Sıçanların serumlarında amilaz, insülin ve
glukoz düzeyleri ölçülmüştür. Alınan pankreas dokularında ise malondialdehit
(MDA) ve myeloperoksidaz (MPO) düzeyleri ölçülmüştür. Ayrıca pankreas dokuları
hematoksilen-eosin (H-E) boyama yöntemi ile histopatolojik olarak değerlendirilmiştir.
Sonuçlar incelendiğinde, LPS’nin sıçanlarda sepsis meydana getirdiği ve
pankreas dokularında hasar oluşturduğu görülmüştür. Ancak, metforminin tedavi
gruplarında bu hasarı önemli ölçüde hafiflettiği tespit edilmiştir. Özellikle,
sıçanlara sepsis oluşturulmadan önce uygulanan metforminin daha etkili olduğu,
pankreas dokularında koruyucu etkilere sahip olduğu gösterilmiştir.

Anahtar Kelimeler

Lipopolisakkarit, Metformin, Pankreas, Sepsis, Sıçan

Early Treatment with Metformin Decreases Pancreatic Damage in Rats with LPS Induced Sepsis

Öz

In this study, the effects of metformin on
pancreatic tissue after lipopolysaccharide (LPS)-induced sepsis were
investigated. 30 Sprague Dawley male rats were used in the study. Five groups were formed: control, sepsis, sepsis+1 hour before
metformin, sepsis+1 hour later
metformin and sepsis+3 hour later metformin as 6 animals in each group. LPS and metformin was prepared at 5 mg/kg and 200 mg/kg
volumes, respectively, and injected intraperitoneally to the rats. Blood samples
and pancreas tissues were
taken from the rats 24 hours after LPS injection. Amylase, glucose and insulin parameters were measured in serum of rats. Malondialdehyde (MDA)
and myeloperoxidase (MPO) parameters in pancreas tissues of rats were
evaluated. Pancreatic tissues
were examined by hematoxylin-eosin (H-E) staining method histopathologically. When the results were evaluated, it was seen that LPS
caused sepsis and pancreatic tissue damage in rats. However, it has been
determined that metformin
significantly alleviates these damages in the treatment groups. In particular, metformin administered prior to sepsis has been shown to have
protective effects in the pancreatic tissues of rats.

Anahtar Kelimeler

Lipopolysaccharide, Metformin, Pancreas, Sepsis, Rat

Kaynakça

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