Daily Dose Dependent Effects of Fluoxetine-HCl (FLX) on Heart Tissue of Zebrafish (Danio rerio Hamilton, 1822)

Yıl 2018, Cilt: 3 Sayı: 2, 272 - 276, 25.12.2018

Figen Esin Batça Kayhan , Şeyma Tartar Kızılkaya Harika Eylül Esmer Duruel , Güllü Kaymak

Öz

The aim of this study is to
investigate the daily dose dependent effects of Fluoxetine-HCl (FLX) on
antioxidant enzymes (CAT, GSH), lipid peroxidation levels (MDA) and total
protein (TP) levels of zebrafish heart tissue. FLX is the active compound of
the antidepressant Prozac™. FLX acts as a selective serotonin reuptake
inhibitor (SSRI) in humans. This study was planned as a model to investigate
the dose dependent effects of daily intake doses of FLX, which adapted from
human proportionally to weight of zebrafish. Biochemical analyses catalase
(CAT), glutatyon (GSH), lipid peroksidasyon levels (MDA) and total protein (TP)
were detected using spectrophotometric methods. In this study, there was not
found any negative effects of FLX on catalase and glutation activity,
malondialdehyde levels and total protein levels of zebrafish heart tissue
during specified periods.

Anahtar Kelimeler

Danio rerio, Fluoxetin-HCl, Heart tissue, Zebrafish

Fluoksetin-HCl’in (FLX) Zebra Balığı (Danio rerio Hamilton, 1822) Kalp Dokusu Üzerine Günlük Doza Bağlı Etkileri

Öz

Bu çalışmanın amacı,
Fluoksetin-HCl’in (FLX) zebra balığı kalp dokusunda antioksidan enzimler (CAT,
GSH), lipid peroksidasyon (MDA) ve total protein (TP) düzeyleri üzerine
insanlarda kullanılan günlük doza bağlı etkilerinin araştırılmasıdır. FLX,
insanlarda antidepresan olarak kullanılan Prozac™’ın etken maddesi ve SSRI
(Selective Serotonine Reuptake Inhibitor) olarak bilinen bir serotonin geri
alım inhibitörüdür. Bu çalışmada FLX’in günlük uygulama dozu zebra balıklarına
adapte edilmiştir. Biyokimyasal analizler olan katalaz (CAT), glutatyon (GSH),
lipid peroksidasyon düzeyleri (MDA) ve total protein (TP) miktarları
spektrofotometrik yöntemlerle belirlenmiştir. Bu çalışmada FLX’in belirlenen
zaman aralıklarında zebra balığı kalp dokusu katalaz ve glutatyon aktivitesi,
malondialdehit seviyeleri ve total protein düzeyleri üzerine olumsuz bir etkisi
saptanmamıştır. 

Anahtar Kelimeler

Danio rerio, Fluoksetin-HCl, Kalp dokusu, Zebra balığı

Kaynakça

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