Antimicrobial Photodynamic Therapy Using Indocyanine Green Loaded FDG Conjugated Cubic Iron Oxide (C-Fe3O4) Nanoparticles

Öz

Our aim is to provide nanostructures combining photodynamic therapy (PDT) and low-dose radiation therapy (RT) to target Escherichia coli ( E. coli) and Green Fluorescence Protein-E coli bacteria (GFP-E. coli). To accomplish this, we synthesized cubic iron oxide nanoparticles conjugated with 2-deoxy-2-[fluorine-19] fluoro-D-glucose (FDG) and loaded them with indocyanine green (ICG) (referred to as ICG@FDG-MNPs). ICG, which has the potential for PDT, was incorporated into ICG@FDG-MNPs to enable effective combined therapy approach targeting E. coli bacteria using an LED beam and LINAC-X-ray source treatment. PDT/RT studies demonstrated that when the nanoconjugate was initially stimulated with the LED beam on E. coli and Green Fluorescence Protein-E. coli bacteria, radiation damage increased, ultimately leading to bacterial death.

Anahtar Kelimeler

• Photodynamic therapy
• superparamagnetic iron oxide nanoparticles (SPIONs)
• near-infrared (NIR)
• Escherichia coli (E.coli)
• Green Fluorescence Protein (GFP)

Destekleyen Kurum

Ege University Nuclear Sciences Institute

Etik Beyan

The authors declare that there is no ethical violation.

Teşekkür

Authors thank Prof Dr Serap Evran from Ege University, Department of Biochemistry in Izmir-Turkey for the green fluorescent bacteria she gifted and Dr. Funda Manalp and Alpman Manalp for LINAC irradiation in Medicana Hospital, Izmir/Turkey.

Kaynakça

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