Farklı Gama Kaynaklarının In Vitro Fraser photinia Sürgün Explantları Üzerine Etkisi ve Etkili Mutasyon Dozunun Belirlenmesi

Öz

Fraser photinia (Photinia × fraseri cv. Red Robin), çevresel dayanıklılığı ve dekoratif özelliği nedeniyle bahçe tasarımlarında süs bitkisi olarak yaygın olarak tercih edilmektedir. Türün mevcut çeşitlerinin değişen iklim koşullarına uyum kapasitesinin artması göz önüne alındığında, pazara çekiciliği yüksek yeni çeşitlerin geliştirilmesi çabaları artmaya başlamıştır. In vitro çoğaltılmaya yatkın olan bu tür için in vitro mutasyon ıslah yöntemi, mevcut değişkenliği genişletmek için ciddi bir potansiyele sahiptir. Fraser photinia üzerinde in vitro mutasyon çalışmaları için iyonlaştırıcı gama ışını kaynaklarının uygulanabilirliğini belirlemek çok önemlidir. Bu çalışmada, in vitro sürgün eksplantlarının radyasyon duyarlılığını belirlemek için iki farklı gama iyonlaştırıcı radyasyon kaynağı sezyum 137 (137Cs) ve kobalt 60 (60Co) kullanılmış ve kaynaklar için etkili mutasyon dozu (EMD50) değerleri belirlenmiştir. Bu amaçla, in vitro sürgün eksplantları on üç farklı dozda (0, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 130 Gy) 60Co (Doz hızı: 235 Gy/saat) ve 137Cs (Doz hızı: 821 Gy/saat) gama ışını kaynaklarıyla ışınlanmıştır. Işınlamadan otuz gün sonra, in vitro bitkiciklerde sürgün uzunluğu ve yaprak sayısı ölçülmüş ve EMD50 değerleri doğrusal regresyon analizi ile hesaplanmıştır. 137Cs kaynak ışını uygulaması sonucu yaprak sayısına dayalı EMD50 dozu 60.34 Gy olarak belirlenirken, 60Co kaynağı için bu değer 80.88 Gy olarak belirlenmiştir. Bu sonuçlara göre kaynak gücünün, ışınlama süresinin ve ışının dokuya nüfuz etmesi sırasında ışının doğrusal enerji transfer değerinin oluşturduğu etkinin EMD50 doz farkı üzerinde etkili olduğu görülmüştür.

Anahtar Kelimeler

• Fraser photinia
• in vitro mutasyon
• etkili mutasyon dozu

Fraser Photinia shoot explantation in vitro: effects of two distinct gamma-ray sources and identification of the optimal mutation dose

Öz

Because of its beautiful qualities and ability to withstand harsh conditions, Fraser photinia (Photinia × fraseri cv. Red Robin) is frequently used as an ornamental plant in garden designs. The efforts to create new, highly marketable variations of the species have begun to increase in response to the growing ability of the current kinds to adapt to changing climatic circumstances. For this species, which is susceptible to in vitro propagation, the in vitro mutation breeding technique holds significant promise for increasing the current variety. It is essential to ascertain whether ionizing gamma ray sources are suitable for in vitro mutation investigations on Fraser photinia. In order to do this, in vitro shoot explants were exposed to a total of thirteen different doses of radiation using 60Co (dosage rate: 235 Gy/h) and 137Cs (dosage rate: 821 Gy/h) gamma ray sources. The number of leaves and shoot length in in vitro plantlets were assessed thirty days after irradiation, and linear regression analysis was used to get the EMD50 values. Based on the quantity of leaves, the EMD50 dose for the cesium-137 source ray application was 60.34 Gy, whereas the cobalt-60 source resulted in an EMD50 dose of 80.88 Gy. These findings demonstrated that the EMD50 dose difference was significantly impacted by the source power, irradiation duration, and the influence produced by the linear energy transfer value of the irradiation during tissue penetration.

Anahtar Kelimeler

• EMD50
• in vitro mutation
• Photinia fraseri
• Red Robin

Destekleyen Kurum

This research was supported by the Margeht Biotechnology Llc. Co.

Etik Beyan

No environmentally harmful application was made in this research.

Teşekkür

This research was supported by the Margeht Biotechnology Llc. Co. The authors express their sincere gratitude to the TENMAK NÜKEN for irradiation treatment.

Kaynakça

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