Antimicrobial Photodynamic Therapy Using Indocyanine Green Loaded FDG Conjugated Cubic Iron Oxide (C-Fe3O4) Nanoparticles

Yıl 2024, Cilt: 37 Sayı: 2, 31 - 40, 09.01.2025

Önder Bakır

Öz

Our aim is to provide nanostructures combining photodynamic therapy (PDT) and low-dose
radiation therapy (RT) to target Escherichia coli ( E. coli) and Green Fluorescence Protein-E coli
bacteria (GFP-E. coli). To accomplish this, we synthesized cubic iron oxide nanoparticles
conjugated with 2-deoxy-2-[fluorine-19] fluoro-D-glucose (FDG) and loaded them with
indocyanine green (ICG) (referred to as ICG@FDG-MNPs). ICG, which has the potential for
PDT, was incorporated into ICG@FDG-MNPs to enable effective combined therapy approach
targeting E. coli bacteria using an LED beam and LINAC-X-ray source treatment. PDT/RT
studies demonstrated that when the nanoconjugate was initially stimulated with the LED beam
on E. coli and Green Fluorescence Protein-E. coli bacteria, radiation damage increased,
ultimately leading to bacterial death.

Anahtar Kelimeler

Photodynamic therapy, superparamagnetic iron oxide nanoparticles (SPIONs), near-infrared (NIR), Escherichia coli (E.coli), Green Fluorescence Protein (GFP)

Etik Beyan

The authors declare that there is no ethical violation.

Destekleyen Kurum

Ege University Nuclear Sciences Institute

Teşekkür

Authors thank Prof Dr Serap Evran from Ege University, Department of Biochemistry in Izmir-Turkey for the green fluorescent bacteria she gifted and Dr. Funda Manalp and Alpman Manalp for LINAC irradiation in Medicana Hospital, Izmir/Turkey.

Kaynakça

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