Determination of antioxidant activity of Medicago sativa L. flowers by biosensor and quantitative analysis of bioactive compounds by LC-MS/MS

Year 2025, Volume: 2 Issue: 1, 9 - 17, 25.06.2025

Ramazan Erenler , İlyas Yıldız , Esma Nur Geçer , İbrahim Hosaflıoğlu , Mehmet Hakkı Alma , Ömer Işıldak

Abstract

Natural products play a vital role in various aspects of human life, particularly in health, agriculture, and industry. These are substances derived from plants, animals, microorganisms, and minerals, and they have been used for centuries. Natural products have historically served as the foundation for many modern medicines. Over 50% of current pharmaceuticals are derived from or inspired by natural compounds. Plants are a rich and vital source of natural antioxidants that protect the body from oxidative stress caused by free radicals. The antioxidant effect of Medicago sativa L. flowers (MSF) was determined by sensory. Moreover, quantitative analysis of phenolics in MSF was presented. MSF scavenged DPPH radical by 41.71%. This value indicates that the plant exhibits a good antioxidant effect. Total phenolic content of MSF was determined to be 92.18 mg GA/g extract. MSF contained kaempferol (88.99), coumarin (24.42), and Kaempferol-3-glucoside (20.39 µg/g extract). Consequently, this plant's flowers include the pharmaceutically critical natural compounds for food and pharmaceutical industries.

Keywords

Medicago sativa , Sensory , Quantitative analysis , Antioxidant activity

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