Asteraceae Ailesine Ait Farklı Türlerin Antibakteriyel Aktivitesinin Belirlenmesi

Yıl 2018, Cilt: 21 Sayı: 2, 31 - 35, 29.12.2018

Dudu Duygu Kılıc Arif Ayar Ceren Baskan Tuba Yıldırım

Öz

Son yıllarda alternatif tıp uygulamalarında bitkilerin kullanımı artmıştır. Sentetik kimyasalların görülen zararlı etkilerinden dolayı
tıbbi bitkilerden elde edilen özütlerin antioksidan, antibakteriyel ve antifungal özellikleri araştırılmaktadır. Bu çalışmada, Amasya
ilinde farklı ekolojik şartlarda yayılış gösteren bazı bitki türlerinin antibakteriyel aktiviteleri araştırılmıştır. Asteraceae ailesine ait
Anthemis tinctorial L., Matricaria chamomilla L. ve Achillea biebersteinii Afan türlerinin metanolik özütleri sokslet cihazı
yardımıyla elde edilmiştir. Elde edilen özütlerin antibakteriyal aktivitesi disk difüzon ve mikrodilüsyon yöntemi ile belirlenmiştir.
Antibakteriyel aktivite için Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa ve
Salmonella enteritidis standart suşları kullanılmıştır. Bu çalışmada, bitki özütleri kontrol olarak kullanılan antibiyotik grubuyla
karşılaştırıldığında antibakteriyel etkiye sahip olduğu gözlemlenmiştir. Disk difüzyon yönteminin sonuçlarına göre, en yüksek
antibakteriyel etki sırasıyla A. tinctorial, M. chamomilla ve A. biebersteinii olarak belirlenmiştir. Mikrodilüsyon yönteminde bitki
özütlerinin konsantrasyon aralığı 6,25 - 50 mg/mL olacak şekilde ayarlanmıştır. Sonuç olarak çalışmada kullanılan bitki özütlerinin
alternatif tedavi amacıyla kullanılabileceği ve daha sonra yapılacak antibakteriyel çalışmalara yön göstereceği düşünülmektedir.

Anahtar Kelimeler

Asteraceae, Disk difüzyon, Mikro dilüsyon

Antibacterial Activity Determination of Different Species Belonging to Asteraceae Family

Öz

The use of herbs as alternative medicine has increased dramatically in the last years. In recent years, due to the harmful effects of
chemical drugs are frequently observed antioxidant, antibacterial and antifungal properties of extracts which derived from plants
and they have been used in medicine. In this study, aim was investigated the antibacterial activities of some weed species which
distributed to ecologically for different conditions. The antibacterial activities of Anthemis tinctorial L., Matricaria chamomilla L.
and Achillea biebersteinii Afan. which belonging to Asteraceae family from Amasya province were determined by using disc
diffusion and micro dilution methods. The methanolic extracts of these weeds were prepared with Soxhlet Extractor. The extracts
were tested against standard strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa
and Salmonella enteritidis. In this study, it was observed that weed extracts had antibacterial effects when they compared with
control antibiotic group. the bacteria to be tested are sensitive to the control group antibiotics. According to the results of disc
diffusion method, the highest antibacterial effect was identified A. tinctorial, M. chamomilla and A. biebersteinii respectively. The
microdilution method was studied concentration range from 6.25 - to 50 mg/mL. In this context, it can be considered that these
weed species are used as an alternative treatment and can lead to later antibacterial studies.

Anahtar Kelimeler

Asteraceae, Disc diffusion, Micro dilution

Kaynakça

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