Larvicidal Toxicity of Vitex agnus-castus L. (Verbenaceae) Extracts to Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Thaumetopoeidae) Larvae

Yıl 2020, Cilt: 23 Sayı: 2, 155 - 163, 31.12.2020

Ayşe Usanmaz Bozhüyük

Öz

In this study, the toxicities of acetone, petroleum ether and methanol extracts of chaste tree (Vitex agnus-castus L.) (Verbenaceae) plant to the pine processionary moth (PPM) (Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Thaumetopoeidae)) larvae in the petri dishes (12x1.5cm) under laboratory conditions were investigated. Each dose was dissolved using acetone, petroleum ether and methanol, and diluted with distilled water. 1 ml of this solution was obtained and sprayed to each instar larvae. Tests were separately performed for five larvae instars and each test was repeated three times. Sterile water+ethanol as the negative control and the commercial active substance chemical Diflubenzuron (25%) as a positive control were used. At 96th h of the exposure, the extracts caused between 20 and 100% mortalities. According to LD25, LD50 and LD90 values, while the most toxic values were recorded as 0.086, 0.154 and 0.001 (for L1; L5; L5) mg/larvae in the petroleum ether extract, the lowest values were 3.684, 8.861 (for L4; L3) in the methanol extract and 754.883 (for L1) mg/larvae in the petroleum ether extract, respectively. The results illustrated that three extracts of V. agnus-castus have a larvicidal potential in the control of PPM larvae in comparison with the controls.

Anahtar Kelimeler

Vites agnus-castus L., Extract, Pine processionary moth, Toxicity

Destekleyen Kurum

yok

Proje Numarası

yok

Teşekkür

Author wolud like to thank Assoc. Prof. Dr. Memiş KESDEK (Mugla Sıtkı Kocman University) for her valuable helps and cooperations during the study, especially the collecting of the biological material.

Vitex agnus-castus L. (Verbenaceae) Ekstraktlarının Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Thaumetopoeidae) Larvalarına Karşı Larvasidal Etkileri

Öz

Bu çalışmada, hayıt bitkisinin (Vitex agnus-castus L.) (Verbenaceae) aseton, petrol eteri ve metanol extraktlarının 0,25, 0,5 ve 1 mg/mL dozlarında, 24., 48., 72. ve 96. saatlerinde çam keseböceği (Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Thaumetopoeidae)) larvalarına karşı laboratuvar şartlarında ve petri kaplarında (12 x 1.5 cm) toksisiteleri incelenmiştir. Her bir doz; aseton, petrol eteri ve metanol kimyasalları kullanılarak çözülmüş ve saf su ile seyreltilmiştir. Bu çözeltinin 1 mL'si alınarak, Petri kaplarındaki her bir larva dönemine püskürtülmüştür. Tüm testler beş larva dönemleri için ayrı ayrı yapılmış, her bir doz için 3’er kez tekrarlanmıştır. Saf su+etanol negatif kontrol ve ticari aktif madde olan kimyasal Diflubenzuron (%25) ise pozitif kontrol olarak kullanılmıştır. Uygulamanın 96. saatinde, bu ekstraktlar %20-100 arasında ölümlere sebep olmuştur. LD25, LD50 ve LD90 değerlerine göre, en yüksek toksik değer L1 için 0.086, L5 için 0.154 ve L5 için 0.001 mg/larva olarak petrol eteri ekstraktında, en düşük toksik değer ise L4 için 3.684, L3 için 8.861 metanol ekstraktında ve L1 için ise 754.883 mg/larva olarak petrol eteri ekstraktında belirlenmiştir. Bu sonuçlar, V. agnus-castus’un üç farklı ekstraktının çam keseböceği larvalarının mücadelesinde larvasidal potansiyele sahip olduklarını göstermiştir. 

Anahtar Kelimeler

hayıt, bitki ekstraktı, çam keseböceği, toksisite

Proje Numarası

yok

Kaynakça

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