The Effects of Omega 3 Fatty Acids on the Healing of Colonic Anastomosis

Yıl 2009, Cilt: 3 Sayı: 1, 15 - 20, 19.03.2009

Saadet Akturan

Öz

The aim of this study is to study the effects of omega 3 fatty acids on colonic anastomosis healing. Four groups that include 6 Wistar albino rats (200-250 g) each were made. In Group I, anastomosis of the colon was made. On the postoperative third day, the level of perianastomotic tissue hydroxiprolin, and anastomosis bursting pressure was recorded. To Group II, 50 mg/kg/days omega 3 fatty acids were given orally for six days. At the end of the third day, colonic anastomosis was made to the rats and the values of the same parameters were recorded. In Group III, the values of the same parameters were investigated seven days after the colonic anastomosis operation. To Group IV, 50 mg/kg/days omega 3 fatty were given orally for ten days. At the end of the third day, colonic anastomosis was made to the rats and the values of the same parameters were recorded on the postoperative seventh day. Even though the bursting pressure of Group II was found to be higher than that of Group I, it was not statistically significant (p> 0.05). The bursting pressure of Group IV was found to be significantly higher than that of Group III (p <0.05). Although the level of perianastomotic tissue hydroxiprolin was higher than control groups in groups to which omega 3 fatty acids were given, it was not found statistically significant (p> 0.05). Since the bursting pressure of Group IV was found to be significantly higher then the other groups; it is suggested that omega 3 fatty acids make colonic anastomosis stronger when it is given longer.

Anahtar Kelimeler

Omega 3 fatty acids, colonic anastomosis, bursting pressure, hydroxyproline

Omega 3 Yağ Asitlerinin Kolon Anastomoz İyileşmesi Üzerine Etkisi (Deneysel Çalışma)

Öz

Bu çalışmada omega 3 yağ asitlerinin kolon anastomoz iyileşmesi üzerine etkilerinin araştırılması amaçlandı. Her biri 6 Wistar Albino rat (200-250 gram) içeren 4 grup oluşturuldu. I. gruba kolon anastomozu yapılarak postoperatif 3. gün anastomoz patlama basıncı, perianastomotik doku hidroksiprolin düzeyine bakıldı. II. gruba 6 gün boyunca günde 50 mg/kg omega 3 yağ asidi oral yolla verildi. 3. gün sonunda ratlara kolon anastomozu yapılarak postoperatif 3. gün aynı parametrelere bakıldı. III. gruba kolon anastomozu yapılarak postoperatif 7. gün aynı parametrelere bakıldı. IV. gruba 10 gün günde 50 mg/kg/gün omega 3 yağ asidi oral yolla verildi. 3.gün sonunda ratlara kolon anastomozu yapılarak postoperatif 7. gün aynı parametrelere bakıldı. II. grubun kolon patlama basıncı değeri I. gruptan yüksek bulunmasına rağmen istatistiksel olarak anlamlı değildi (p> 0.05). IV. grubun kolon patlama basıncı değeri III. gruptan istatiksel anlamlı yüksek bulundu (p< 0.05). Omega 3 yağ asidi verilen grupların kontrol gruplarına göre perianastomotik doku hidroksiprolin değerleri yüksek olmasına rağmen istatistiksel olarak anlamlı bulunmadı (p> 0.05). Uzun süreli omega 3 yağ asidi verilen radarda postoperatif 7. günde kolon patlama basıncının anlamlı yüksek bulunması omega 3 yağ asitinin anastomozun sağlamlığını arttırdığını düşündürmüştür.

Dr. Önder ÖZERJ,
Dr. Adnan HASANOĞLU,
Dr. Saadet AKTURAN,
Dr. Osman GÜLER,
Dr. Doğan YÜCEL,

Anahtar Kelimeler

Omega 3 yağ asitleri, kolon anastomozu, patlama basıncı, hidroksiprolin

Kaynakça

• 1. Rolandelli RH, Koruda MJ, Setle R.G, Rom-beau JL. The effect of enteral feedings supplemented with pectin on the healing of colonic anastomoses in the rat. Surgery. 1986; 99: 703-7.
• 2. Doğru O, Hasanoğlu A, şakrak Ö, Koç M, Kama NA. Kolon travmalarında primer onarım. Kolon Rektum Hast Dergisi. 1991; 2: 78-86.
• 3. Irwin ST, Krukowski ZH, Matheson NA. Single layer anastomosis in the upper gastrointestinal tract. Br J Surg. 1990; 77: 643-4.
• 4. Williams NS. Surgical Principles. In: Keighley MRB, Wiliams HS;eds. Surgery of anus, rectum and colon. Philadelphia, Saunders, 1993: 102-127.
• 5. Gündoğdu H. Cerrahi hastada beslenme des- teği. Cerrahi Tıp Bilimler Dergisi. 2003;3-21.
• 6. Bonis PA, Chung M, Tatsioni A et al. Effects of omega 3 fatty acids on organ transplantation. Evid Rep Technol Assess. 2005; 115:1-11.
• 7. Rolandelli RH, Koruda MJ, Settle RG, Rom-beau JL. Effects of intraluminal infusion of short-chain fatty acids on the healing of colonic anastomosis in the rat. Surgery. 1986; 2: 198-204.
• 8. Jiborn H, Ahonen J, Zedefeldt B. Healing of experimental colonic anastomoses. Am J Surg. 1978;136:587-94
• 9. Bozzetti F, Terno G, Longoni C. Parenteral hyperalimentation and wound healing. Surg Gynecol Obstet. 1975; 141:712-4.
• 10. Subbaiah PV, Kaufman D, Bagdade JD. Incorporation of dietary n-3 fatty acids into molecular species of phosphatidyl choline and cholesteryl ester in normal human plasma. Am J Clin Nutr. 1993 ;58 :360-8.
• 11. Kinsella JE. Lipids, membrane receptors, and enzymes: effects of dietary fatty acids. J Parenter Enteral Nutr. 1990; 14: 200-217.
• 12. Pomposelli JJ, Mascioli EA, Bistrian BR, Lopes SM, Blackburn GL. Attenuation of the febrile response in guinea pigs by fish oil enriched diets. J Parenter Enteral Nutr. 1989; 13:136-140.
• 13. Jelen S, Tempel G, Lohninger A, Blümel G. Changes in essential fatty acids in plasma lipid fractions of traumatized patients. J Parenter Enteral Nutr. 1983; 7:142-5.
• 14. Gerster H. The use of n-3 PUFAs (fish oil) in enteral nutrition. Int J Vitam Nutr Res. 1995; 65: 3-20.
• 15. Foitzik T, Eibl G, Schneider P, Wenger FA, Jacobi CA, Buhr HJ. Omega-3 fatty acid supplementation increases anti-inflammatory cytokines and attenuates systemic disease sequelae in experimental pancreatitis. Foitzik T, Eibl G, Schneider P, Wenger FA, Jacobi CA, Buhr HJ. J Parenter Enteral Nutr. 2002; 26: 351-6.
• 16. Bjerve KS, Fischer S, Wammer F, Egeland T. Alpha-Linolenic acid and long-chain omega-3 fatty acid supplementation in three patients with omega-3 fatty acid deficiency: effect on lymphocyte function, plasma and red cell lipids, and prostanoid formation. Am J Clin Nutr. 1989; 49: 290-300.
• 17. Holman RT, Johnson SB, Hatch TF. A case of human linolenic acid deficiency involving neurological abnormalities. Am J Clin Nutr. 1982; 35:617-23.
• 18. Daly JM, Lieberman MD, Goldfine J, Shou J, Weintraub F, Rosato EF, Lavin P. Enteral nu- trition with supplemental arginine, RNA, and omega-3 fatty acids in patients after operation: immunologic, metabolic, and clinical outcome. Surgery. 1992; 112:56-67.
• 19. Cerra FB, Lehmann S, Konstantinides N et al. Improvement in immune function in ICU patients by enteral nutrition supplemented with arginine, RNA, and menhaden oil is independent of nitrogen balance. Nutrition. 1991; 7: 193-9.
• 20. Kaya Y, Yurttaş O. Enteral immünnutrisyonun deneysel yara iyileşmesine etkileri. Ulusal Cerrahi Dergisi. 2000; 6:299-306
• 21. Senkal M, Mumme A, Eickhoff U et al. Early postoperative enteral immunonutrition: clinical outcome and cost-comparison analysis in surgical patients. Crit Care Med. 1997;25(9): 1489-96.
• 22. Whiting CV, Bland PW, Tarlton JF. Dietary n-3 polyunsaturated fatty acids reduce disease and colonic proinflammatory cytokines in a mouse model of colitis. Inflamm Bowel Dis. 2005;11:340-9.
• 23. Gerçek A, Yildirim Ö, Konya D ve ark. Effects of parenteral fish-oil emulsion (omegaven) on cutaneous wound healing in rats treated with dexamethasone. Journal of Parenteral and Enteral Nutrition. 2007; 31:161-166.
• 24. Hendrickse CW, Keighley MR, Neoptolemos JP. Dietary omega-3 fats reduce proliferation and tumor yields at colorectal anastomosis in rats. Gastroenterology. 1995; 109: 431-9.
• 25. Jiborn H, Ahonen J, Zederfeldt B. Healing of experimental colonic anastomoses. III. Collagen metabolism in the colon after left colon resection. Am J Surg 1980; 39: 398-405.