The Effects of Montelukast Against Methotrexate-Induced Acute Renal Damage

Abstract

Objective: The effects of montelukast against methotrexate-induced renal damage were investigated. Materials and Methods: 35 Wistar albino female rats were divided into 5 groups as follows: Group I: Control; Group II: Montelukast (ML); Group III: Methotrexate (Mtx); Group IV: Montelukast treatment after methotrexate application (Mtx+ML); Group V: Montelukast treatment before methotrexate application (ML+Mtx). At the end of the experiment, the kidney tissues and the blood of rats were collected. Malondialdehyde (MDA) levels were determined from kidney tissues. Blood urea nitrogen (BUN) and creatinine (Cr) were assessed in the serum. In addition the kidney tissues were examined histologically. Results: MDA, BUN and Cr levels of of group III significantly increased when compared to groups I and II. These parameters of group IV decreased when comparet to group III. The significant tubul damages were observed in only methotrexate injection group (group III) histologically. In addition, montelukast treatment (in groups IV and V) reduced these histological damages. Conclusion: It was showed that montelukast treatment after methotrexate application could reduce methotrexate-induced experimental renal damage. Key words: Methotrexate; Montelukast; Kidney; Rat.

Keywords

• Methotrexate; Montelukast; Kidney; Rat.

Metotreksat’ın Neden Olduğu Akut Böbrek Hasarına Karşı Montelukast’ın Etkileri

Öz

Amaç: Metotreksat'ın neden olduğu böbrek hasarına karşı montelukast'ın etkileri araştırıldı. Gereç-Yöntem: 35 adet Wistar albino dişi sıçan 5 gruba ayrıldı: Grup I: Kontrol (K); Grup II: Montelukast (ML); Grup III: Metotreksat (Mtx); Grup IV: Önce metotreksat sonra montelukast verilen grup (Mtx+ML); Grup V: Önce montelukast sonra metotreksat uygulanan grup (ML+Mtx) olacak şekilde ayarlandı. Son uygulamalardan 24 saat sonra hayvanlar sakrifiye edilerek böbrek dokuları ve kanları alındı. Böbrek dokuları hem histolojik incelemeler hem de MDA analizi için kullanıldı. Kandan elde edilen serumlarda ise BUN ve Cr seviyeleri değerlendirildi. Bulgular: Sadece metotreksat uygulanan grupta kontrol grubuna gore MDA, BUN ve Cr seviyelerinde anlamlı bir artış tespit edildi. Bunun yanı sıra metotreksat enjeksiyonundan sonra montelukast verilen grupta bu parametreler düşmüştü. Histolojik olarak sadece metotreksat verilen grupta belirgin tübül hasarı varken montelukastın her iki uygulaması ise bu hasarları geriletmişti. Sonuç: Metotreksat'ın neden olduğu deneysel böbrek hasarında, montelukast'ın bu hasarı azalttığı ortaya kondu. Anahtar kelimeler: Metotreksat; Montelukast; Böbrek; Rat.

Anahtar Kelimeler

• Metotreksat; Montelukast; Böbrek; Rat.

Kaynakça

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