Comparative Effects of Ovariectomy and Flutamid on Body-Uterus Weight and Uterine Histology in the Ovariectomized Rat Model+

Abstract

Objective: To determine the effect of flutamide on uterine histology and to ascertain whether flutamide regulates body weight gain in the ovariectomized rat model. Materials and Methods: We used 36 sexually mature female Wistar-Albino rats in this study. The study was performed in the Department of Medical Science Application and Research Centre of Dicle University, Diyarbakir. We divided the model rats into 3 groups: group 1- control group, group 2- sham-operated group and group 3- bilaterally ovariectomized group. In addition, we gave flutamide to group 3. Results: We found that the body weight was higher in the ovariectomized rats than the control rats, unlike the decreased uterine net weight. Furthermore, body weight decreased slightly after flutamide therapy, however uterine net weight increased. After flutamide applications, the length of uterinal epithelium, the number of uterinal glands, infiltration of the stromal cells increased and the lumen of uterinal glands were dilateded. Conclusion: As a result, an improvement was observed after flutamide applications while bilateral ovariectomy was determined to cause atrophy in the uterine epithelium of rat. Our study may be source for further research examining the relationship between the androgen receptor antagonist flutamide and reproductive function in female rats. Key words: Flutamide, Anti-androgen, Ovariectomy, Uterine histology, Body weight

Keywords

• Flutamide, Anti-androgen, Ovariectomy, Uterine histology, Body weight

Ovarektomize Ratlarda Flutamid Uygulamasının Vücut Ağırlığı ve Uterus Histolojisi Üzerine Etkilerinin Karşılaştırılması

Öz

Amaç: Ovarektomize ratlarda Flutamid kullanımının vücut ağırlığı ve uterus dokusu üzerine etkilerinin araştırılması. Materyal ve Metod: 36 adet Wistar Albino türü dişi sıçanlar Dicle üniversitesi DÜSAM merkezinden seçildi. Sıçanlar 3 guruba ayrıldı. Grup 1 - kontrol grubu, grup 2 - sham-operasyon, grup 3-bilateral ovarektomi ve flutamid verilen grup. Bulgular: Çalışmada ovarektomize ratların vücut ağırlığı kontrol ratlardan daha fazla bulunurken, uterus ağırlığının azaldığı gözlemlenmiştir. Bununla birlikte flutamid kullanımı sonrasında vücut ağırlığı çok önemli olmamakla beraber azalırken uterus ağırlığı artmıştır. Flutamid uygulamasından sonra uterus epitelinin yüksekliği, uterinal bezlerin sayısı artarken bezlerin lumenlerinde genişleme gözlemlenmiştir. Sonuç: Bilateral ovarektomi ratların uterus epitelinde atrofi şekillendirirken flutamid uygulamasının düzeltici etkilerinin olduğu gözlemlenmiştir. Bu çalışmada bir androjen reseptörü olan flutamid ile dişi ratların üreme fonksiyonları arasındaki ilişkinin daha ilerki çalışmalara kaynak oluşturabileceği görüşündeyiz. Anahtar kelimeler: Flutamid, Anti-androjen, Ovarektomi, Uterus histolojisi, Vücut ağırlığı.

Anahtar Kelimeler

• Flutamid, Anti-androjen, Ovarektomi, Uterus histolojisi, Vücut ağırlığı.

Kaynakça

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40. Corresponding Author
41. Dr. BernaGüney SARUHAN
42. Departments of Histology and Embryology,
43. Veterinary Medicine Faculty, University of Dicle, Diyarbakir
44. Tel : [phone]-8627
45. Tel : [phone]
46. E-Mail : [e-mail]