Effect Of L-Deprenyl On Nitric Oxide Metabolites In Parkinson’s Disease Patients

Öz

Objectives: L-deprenyl is an irreversible mitochondrial monoamine oxidase type-B (MAO-B) inhibitor and is mostly used in Parkinson's Disease (PD) treatment. However, it is believed that the effect of L-deprenyl in PD is by a different mechanism than MAO-B enzyme inhibition and may be due to several factors. Neuroprotective effect of L-deprenyl on cerebral tissue achieved by NO production has been shown by in vivo and in vitro tissue studies. The purpose of this study was to determine the level of nitric oxide (NO) metabolites, nitrite and nitrate in human serum and to assess whether there is any relationship among serum nitrate–nitrite levels and L-deprenyl in PD. Patients and Methods: Twenty-one patients used L-Deprenile and 16 patients did not. Twenty-four healthy age- and sex matched control were included in the study. Serum total nitrite and nitrate levels were determined spectrophotometrically. Results: The levels of Total nitrite and Nitrate in L-Deprenil using and L-Deprenil not using, and control groups were found to be 63.8±30.1 μmol/L and 67.3±29.0 μmol/L, 62.6±16.2 μmol/L and 59.5±16.2 μmol/L, 66.2±21.8 μmol/L and 62.4±21.2 μmol/L, respectively. Serum total nitrite and nitrate levels were not significantly different among the three groups. Conclusion: These data do not support the concept that neuroprotective effect of L-deprenyl on cerebral tissue is achieved by NO production. Therefore, further in vivo and in vitro studies are needed to exactly clarify the cerebral effects of L-deprenyl and the role of NO in patients with PD. Key words: Parkinson's Disease; L-Deprenyl; Nitric Oxide; Total Nitrite; Nitrate.

Anahtar Kelimeler

• Parkinson's Disease; L-Deprenyl; Nitric Oxide; Total Nitrite; Nitrate.

Parkinson Hastalarinda L-Deprenilin Nitrik Oksit Metabolitlerine Etkisi+

Öz

Amaç: L-deprenil, Parkinson hastalığı tedavisinde sıklıkla kullanılan, irreversibl bir mitokondriyal monoamin oksidaz tip-B (MAO-B) inhibitörüdür ve etkisini MAO-B enzim inhibisyonundan farklı bir mekanizma ile yaptığına inanılmaktadır. L-deprenilin serebral dokudaki nöroprotektif etkisini Nitrik oksit (NO) üretimi yoluyla yaptığını düşündüren, in vivo ve in vitro doku çalışmaları yayınlanmıştır. Bu çalışmanın amacı, Parkinson hastalığında LDeprenil kullanımının NO metabolitlerine etkisini araştırmaktır. Gereç ve Yöntem: Bu çalışmaya L-deprenil kullanan (n=21) ve kullanmayan (n=16) olgular ile yaş ve cinsiyet açısından eşleştirilmiş sağlıklı kontrol grubu (n=24) dahil edildi. Serum total nitrit ve nitrat düzeyleri spektrofotometrik olarak belirlendi. Sonuçlar: L-Deprenil kullanmayan grupta; Total nitrit:62.6±16.2 μmol/L, Nitrat:59.5±16.2 μmol/L, L-Deprenil kullanan grupta; Total nitrit: 63.8±30.1 μmol/L, Nitrat: 67.3±29.0 μmol/L, kontrol grubunda; Total nitrit:66.2±21.8 μmol/L, Nitrat:62.4±21.2 μmol/L bulundu. Serum total nitrit ve nitrat düzeyleri üç grup arasında istatistiksel olarak farklı değildi. Tartışma: Bu bulgular, L-deprenilin serebral dokudaki nöroprotektif etkisinin NO üretimi yoluyla olduğunu ileri süren görüşü desteklememiştir. L-deprenilin serebral doku ve NO metabolizmasına etkisini anlayabilmek için in vivo ve in vitro farklı çalışmalara ihtiyaç vardır. Anahtar kelimeler: Parkinson hastalığı; L-Deprenil; Nitrik oksit; Total nitrit; Nitrat.

Anahtar Kelimeler

• Parkinson hastalığı; L-Deprenil; Nitrik oksit; Total nitrit; Nitrat.

Kaynakça

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