The effect of topical ascorbic acid application on the healing of rat tympanic membrane perforations

Yıl 2003, Cilt: 11 Sayı: 1, 1 - 4, 17.01.2003

Uğur Çınar Özgür Yiğit Berna Uslu Coşkun Seyhan Alkan Özlem Ünsal Gökhan Akgül Burhan Dadaş Tülay Başak

Öz

Objectives: We investigated the effect of topical administration of ascorbic acid on the healing process of tympanic membrane perforations in rats.Design and Methods: Thermal myringotomy was induced in both ears of 22 albino rats. The right and left ears were assigned to topical applications of ascorbic acid and şaline solution, respectively. The same procedure was performed after 48 hours.Perforations were examined daily by otomicroscopy and healing periods were determined. For histopathologic examination, a single rat in which closure of the tympanic membranes was not com- pleted was sacrificed on days 5, 7, 10, and 12. Data were analyzed with the use of the Student’s t-test.Results: The mean durations of healing in the right and left ears were 7.6 and 8.3 days, respectively. No significant differences were found betvveen the applications of ascorbic acid and şaline solution with regard to the duration of closure and histopathologic healing criteria.Conclusion: Our data suggest that topical applica­ tion of ascorbic acid have no beneficial effect on the healing of rat tympanic membrane perforations.

Anahtar Kelimeler

Administration, topical, ascorbic acid/therapeutic use, rats, tympanic membrane/pathology/drug effects, tympanic membrane perforation/pathology/drug effects, wound healing/drug effects

Topikal uygulanan askorbik asidin sıçan kulak zarı perforasyonlarında iyileşmeye etkisi

Öz

Amaç: Sıçan kulak zarında oluflturulan perforasyonlarda topikal uygulanan askorbik asidin kulak zarınınkapanma süresi ve iyileflmesine etkisi arafltırıldı.Çalıflma Planı ve Yöntemler: Deneysel olarak oluflturulan termal miringotomi sonrasında 22 sıçanınsağ kulak zarına topikal askorbik asit, sol kulak zarına ise serum fizyolojik uygulandı. İfllem 48 saat sonra tekrarlandı. Sıçanların ameliyat mikroskobu ilegünlük otoskopik muayeneleri yapıldı ve perforasyonların kapanma süreleri belirlendi. Kulak zarlarının her ikisi de kapanmamıfl olan birer sıçanın yaflamı 5, 7, 10 ve 12. günlerde sonlandırıldı ve iyileflmehistopatolojik olarak değerlendirildi. Veriler Studentt-testi ile yorumlandı.Bulgu l a r : Askorbik asit grubunda kulak zarlarının ortalama kapanma süresi 7.6 gün, serum fizyolojik grubunda 8.3 gün bulundu. İki grup arasında kulak zarlarının kapanma süreleri ve histopatolojik iyileflme ölçütleri açısından anlamlı farklılık bulunmadı.Sonuç: Topikal olarak uygulanan askorbik asidin sıçan kulak zarındaki perforasyonun iyileflmesi üzerine etkili olmadığı sonucuna varıldı

Anahtar Kelimeler

Uygulama, topikal, askorbik asit/terapötik kullanım, sıçan, timpanik membran/patoloji/ilaç etkisi, timpanik membran perforasyonu/patoloji/ilaç etkisi, yara iyileflmesi/ilaç etkisi

Kaynakça

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