The effect of vitamin E on histopathologic healing and lipid peroxidation levels in experimentally induced traumatic tympanic membrane perforations

Yıl 2003, Cilt: 10 Sayı: 3, 87 - 92, 01.06.2003

Nihat Susaman Şinasi Yalçın İrfan Kaygusuz Turgut Karlıdağ Üzeyir Gök Nevin İlhan İbrahim Hanifi Özercan

Öz

Objectives: To examine the favorable effects of vitamin E on tympanic membrane perforations induced mechanically in guinea pigs.Study Design: Bilateral tympanic membrane perforations of 1.8 mm were induced in 40 guinea pigs. The animals were ran- domly divided into two groups equal in number. One group remained untreated, while the othervvas administered vitamin E 100 mg/kg/day through intramuscular injections. On days 1,3, 5, and 7, five animals in each group were randomly sacrificed.Histopathologic changes in the tympanic membranes were evaluated and malondialdehyde levels were determined.Results: Significant increases were observed in epithelial thickness, fibroblastic proliferation, and neovascularization in the study group p<0.05 . Epithelial thickness was found to be increased in both groups beginning from the first day; however, this increase was more rapid in the study group. Although malondialdehyde levels showed significant increases on days 3 and 5 in both groups p<0.05 , they returned to the first day values in vitamin E-treated animals on day 7, vvhereas Con­ trols stili maintained high malondialdehyde levels.Conclusion: Vitamin E hastens the healing process of traumatic tympanic membrane perforations.

Anahtar Kelimeler

Antioxidants, guinea pigs, lipid peroxides/metabolism, malondialdehyde/metabolism, tympanic membrane/injuries/drug effects, vitamin E/administration & dosage, wound healing/drug effects

Travmatik timpanik membran perforasyonlarında E vitamininin histopatolojik iyileşme ve lipid peroksidasyon düzeyleri üzerine etkileri: Deneysel çalışma

Öz

Amaç: E vitamininin, mekanik etkiyle oluflturulan timpanikmembran perforasyonlarında iyileflme üzerine olası olumlu etkilerini ortaya koymak. Çal ı flma Planı: Kırk adet guinea pig’in her iki timpanik membranında, standart 1.8 mm’lik perforasyon oluflturuldu. Denekler 20’flerli iki gruba ayrıldı. Bir gruba tedavi uygulanmazken,diğer gruba 100 mg/kg/gün E vitamini intramusküler enjeksiyonla verildi. Perforasyon oluflturulduktan sonra, 1, 3, 5 ve 7.günlerde gruplardan rastgele seçilen befl kobayın yaflamısonlandırıldı. Timpanik membrandaki histopatolojik değifliklikler değerlendirildi ve doku malondialdehid düzeyleri ölçüldü. Bulgular: Epitel kalınlığı, fibroblast proliferasyonu ve neovaskülarizasyon çalıflma grubunda anlamlı derecedefarklı bulundu p

Anahtar Kelimeler

Antioksidan, guinea pig, lipid peroksitleri/metabolizma, malondialdehid/metabolizma, timpanik membran/yaralanma/ilaç etkisi, vitamin E/uygulama ve doz, yara iyileflmesi/ilaç etkisi

Kaynakça

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