Comparison of adenoid and tonsil core cultures in chronic adenotonsillitis

Yıl 2003, Cilt: 10 Sayı: 3, 105 - 109, 01.06.2003

Sinan Kocatürk Armağan İncesulu Ünsal Erkam Tayfur Demiray Ebru Kandıralı Ali Mert

Öz

Objectives: Tonsil and adenoid core cultures were compared and beta-lactamase producing bacteria were determined in patients with chronic adenotonsillitis.Patients and Methods: Thirty-two patients 21 boys, 11 giriş; mean age 5 years with chronic ade­ notonsillitis underwent elective adenotonsillectomy.The core swaps of tonsil and adenoid tissues were obtained under sterile conditions and were inoculated in %5 sheep blood ağar, eosin methylene blue ağar, and chocolate ağar plates. The frequency of beta-lactamase producing isolates were assessed.Results: Staphylococcus aureus was the most common pathogen both in tonsil and adenoid core cultures.Among pathogenic bacteria isolated from tonsil n=27 and adeonid n=22 cultures, the same strains were iso­lated in 20 cultures 75% . The frequencies of beta-lac­ tamase producing bacteria were 44% and 41% in tonsil and adenoid core cultures, respectively. S. aureus was associated with beta-lactamase production in 88% and 100% in tonsil and adenoid tissues, respectively.Conclusion: Differences betvveen tonsil surface and core bacterial flora may have implications in the etiopathogenesis and treatment of chronic tonsillitis.The range of species isolated seems to be similar for both chronic tonsillitis and adenoiditis.

Anahtar Kelimeler

Adenoids/surgery/microbiology, antibiotics, bacteria/isolation & purification, bacterial infections, betalactamases, child, Staphylococcus aureus, tonsil/microbiology, tonsillectomy, tonsillitis/microbiology/drug therapy/surgery

Kronik adenotonsillitli olgularda adenoid ve tonsil merkez kültürlerinin karşılaştırılması

Öz

Amaç: Kronik adenotonsillitli hastalarda tonsil veadenoid merkez kültürleri karflılafltırıldı ve beta laktamaz üreten bakteriler belirlendi. Hastalar ve Yöntemler: Kronik adenotonsillitli 32hastaya 21 erkek, 11 kız; ort. yafl 5 elektif adenotonsillektomi yapıldı. Tonsil ve adenoid dokusundansteril flartlarda alınmıfl merkez sürüntü kültürleri,aerobik üreme için %5 oranında koyun kanlı agar,eosin metilen mavi agar, çikolatalı agar besiyerlerineekildi. Patojen bakterilerde beta-laktamaz üretmeoranları incelendi. Bulgular: Tonsil ve adenoid merkez kültürlerinde ensık rastlanan patojen Staphylococcus aureus o l a r a kbelirlendi. Tonsil merkez kültürlerinde üreyen 27, adenoid merkez kültürlerinde üreyen 22 patojenin 20’s i n i n %75 aynı olduğu görüldü. Beta-laktamaz üreten bakterilerin oranı, tonsil merkez kültürlerinde %44, adenoid merkez kültürlerinde %41 bulundu. S. aureus’un tonsil merkez kültürlerinde %88, adenoid merkez kültürlerinde %100 oranında beta-laktamaz ürettiği görüldü.Sonuç: Kronik tonsillitte yüzey ve merkez kültürlerinden elde edilen farklı etkenler, hastalığın etyopatogenezi ve tedavisi yönünden önemli olabilir. Ayrıcakronik tonsillit ve adenoidit etkenleri birbirine benzemektedir

Anahtar Kelimeler

Adenoid/cerrahi/mikrobiyoloji, antibiyotik, bakteri/izolasyon ve purifikasyon, bakteriyel enfeksiyon, betalaktamaz, çocuk, Staphylococcus aureus, tonsil/mikrobiyoloji, tonsillektomi, tonsillit/mikrobiyoloji/ilaç tedavisi/cerrahi.

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