The effect of systemic prednisolone on propylene glycol-induced otitis media in guinea pig

Yıl 2006, Cilt: 16 Sayı: 5, 214 - 220, 14.10.2006

Fatma Tülin Kayhan Zeynep Algün

Öz

Objectives: İn this histopathologic study, we investi- gated the effect of systemic prednisolone on propylene glycol-induced otitis media in guinea pig ear.Materials and Methods: Ten adult guinea pigs received systemic prednisolone 10 mg, İM, single dose together with a single application of 60% propy­ lene glycol 0.2 mİ, per ear to both middle ears. Ten control guinea pigs were given a single application of 60% propylene glycol 0.2 mİ in the left ear, and a sin­ gle application of şaline 0.2 mİ in the right ear. Four weeks later, the animals were sacrificed and temporal bones were removed and prepared for histological evaluation. Paraffin-embedded specimens were hori- zontally sectioned at 7 microns. One of each five sec- tions was stained with hematoxylin and eosin, and studied under light microscopy.Results: İn the control group, otitis media developed in six of nine propylene glycol-administered ears 66% , whereas ali saline-treated ears remained intact. İn the prednisolone group, only one 6% of 16 ears developed otitis media and cholesteatoma. The difference was statistically significant.Conclusion: Our results suggest that systemic prednisolone has a potent effect to prevent propy­ lene glycol-induced otitis media in guinea pig ears.

Anahtar Kelimeler

Otitis media/chemically induced, prednisolone/therapeutic use, propylene glycols/toxicity

Guinea pigde propilen glikol ile oluşturulan otitis mediada sistemik prednisolonun etkisi

Öz

Amaç: Bu çalışmada guinea pig orta kulağında propilen glikol ile oluşturulan otitis mediada sistemikprednisolonun etkisi histopatolojik olarak araştırıldı.Gereç ve Yöntem: Çalışma grubunda sistemikprednisolon 10 mg, İM, tek doz uygulanan 10 adeterişkin guinea pigin her iki ortakulağına tek doz, 0.2ml %60 propilen glikol verildi. Kontrol grubundaki 10guinea pigin sol kulaklarına tek doz 0.2 ml %60 propilen glikol, sağ kulaklarına tek doz 0.2 ml saline uygulandı. Propilen glikol uygulamasından dört haftasonra hayvanların yaşamı sonlandırıldı. Çıkarılantemporal kemikler histopatolojik inceleme içinhazırlandı. Parafine gömülen örneklerden horizontalplanda yedi mikronluk kesitler alındı. Her beş kesitten biri hemotoksilen-eosin ile boyanıp, ışık mikroskobu ile incelendi.Bulgular: Kontrol grubunda saline uygulanan kulaklarda patolojik değişiklikler görülmezken, propilenglikol uygulanan dokuz kulağın altısında %66 otitismedia gelişti. Prednisolon grubunda ise 16 kulağınsadece birinde %6 otitis media ve kolesteatomagelişimi gözlendi. Bu fark anlamlı bulundu.Sonuç: Sonuçlarımız, guinea pig kulağında propilenglikol ile oluşturulan otitis medianın sistemik prednisolon tedavisiyle önlenebileceğini göstermiştir

Anahtar Kelimeler

Otitis media/kimyasal yolla oluşturulan, prednizolon/terapötik kullanım, propilen glikol/toksisite

Kaynakça

• Jove MA, Vassalli L, Raslan W, Applebaum EL. The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears. Am J Otolaryngol 1990;11:5-9.
• Kim HJ, Chole RA. Experimental models of aural cholesteatomas in Mongolian gerbils. Ann Otol Rhinol Laryngol 1998;107:129-34.
• Wright CG, Meyerhoff WL, Burns DK. Middle ear cholesteatoma: an animal model. Am J Otolaryngol 1985;6:327-41.
• Vassalli L, Harris DM, Gradini R, Applebaum EL. Inflammatory effects of topical antibiotic suspensions containing propylene glycol in chinchilla middle ears. Am J Otolaryngol 1988;9:1-5.
• Masaki M, Wright CG, Lee DH, Meyerhoff WL. Experimental cholesteatoma. Epidermal ingrowth through tympanic membrane following middle ear application of propylene glycol. Acta Otolaryngol 1989;108:113-21.
• Meyerhoff WL, Wright CG, Gerken GM. Effects of middle ear ventilation on cholesteatoma development in experimental animals. Acta Otolaryngol 1990; 110:279-85.
• Pownell PH, Wright CG, Robinson KS, Meyerhoff WL. The effect of cyclophosphamide on development of experimental cholesteatoma. Arch Otolaryngol Head Neck Surg 1994;120:1114-6.
• White SJ, Wright CG, Robinson KS, Meyerhoff WL. Effect of topical hyaluronic acid on experimental cholesteatoma. Am J Otolaryngol 1995;16:312-8.
• Sennaroglu L, Ozkul A, Gedikoglu G, Turan E. Effect of intratympanic steroid application on the develop- ment of experimental cholesteatoma. Laryngoscope 1998;108(4 Pt 1):543-7.
• Wright CG, Bird LL, Meyerhoff WL. Effect of 5-fluo- rouracil in cholesteatoma development in an animal model. Am J Otolaryngol 1991;12:133-8.
• Kayhan FT, Algün Z. Guinea pig orta kulağına uygula- nan propylene glycol’ün etkisi. Otoskop 2001;3:111-4.
• Morizono T, Paparella MM, Juhn SK. Ototoxicity of propylene glycol in experimental animals. Am J Otolaryngol 1980;1:393-9.
• Wright CG, Bird LL, Meyerhoff WL. Tympanic mem- brane microstructure in experimental cholesteatoma. Acta Otolaryngol 1991;111:101-11.
• Imamura S, Nozawa I, Imamura M, Murakami Y. Pathogenesis of experimental aural cholesteatoma in the chinchilla. ORL J Otorhinolaryngol Relat Spec 1999;61:84-91.