Evaluation of Ki-67 expression in recurrent cases of cholesteatoma

Yıl 2007, Cilt: 17 Sayı: 2, 65 - 69, 18.03.2007

Arif Şanlı İlter Tezer H. Mustafa Paksoy Sedat Aydın Ümit Hardal Nagehan Barşık Özdemir

Öz

Objectives: Recurrences are stili a challenge despite appropriate techniques in cholesteatoma surgery. This study was designed to evaluate the level of Ki-67 expres- sion in recurrent cases of cholesteatoma.Patients and Methods: The study included 32 patients 18 males, 14 females; mean age 34 years; range 12 to 63 years who unden/vent surgery for otitis media. Of these, 19 patients had cholesteatoma, and eight patients had recurrent cholesteatoma. Five patients who unden/vent tympanoplasty for chronic otitis media comprised the con- trol group. AH the patients with cholesteatoma unden/vent radical mastoidectomy. At surgery, tissue samples of cholesteatoma vvere taken and prepared for immunohisto- chemical staining. İn Controls, retroauricular skin samples vvere used. The tvvo patient groups vvith cholesteatoma vvere compared vvith respect to Ki-67 expression.Results: Increased cellular proliferation vvas detected in both groups of cholesteatoma. No significant differ- ence vvas found betvveen tvvo cholesteatoma groups vvith respect to Ki-67 staining p>0.05 . Compared to the Controls, patients vvith cholesteatoma and those vvith recurrent cholesteatoma had significantly higher levels of Ki-67 staining p<0.05 and p<0.01, respec- tively .Conclusion: Our results suggest that, despite a higher degree of proliferation in recurrent cholesteatoma cases, treatment failures may be mainly associated vvith the sur- gical technique, accompanying infections, and the type of cholesteatoma.

Anahtar Kelimeler

Cholesteatoma middle ear, Ki-67 antigen, immunohistochemistry

Tekrarlayan kolesteatomlu olgularda Ki-67 ekspresyonunun değerlendirilmesi

Öz

Amaç: Kolesteatom tedavisinde uygun cerrahi tekniklererağmen nüks izlenmesi günümüzde hala bir sorundur. Buçalışmada, tekrarlayan kolesteatomlarda Ki-67 ekspresyonunun düzeyi araştırıldı.Hastalar ve Yöntemler: Çalışmaya, otitis media nedeniyle ameliyat edilen 32 hasta alındı. Olguların 19’u kolesteatomlu, sekizi tekrarlayan kolesteatomlu kronik otitis medianedeniyle ameliyat edildi. Kronik otitis media nedeniyletimpanoplasti yapılan beş hasta ise kontrol grubu olarakayrıldı. Kolesteatomlu hastaların hepsine radikal mastoidektomi yapıldı. Ameliyat sırasında kolesteatom dokusundan alınan parçalar immünohistokimyasal boyamaişlemi için hazırlandı. Kontrol grubunda retroaurikülerderi örnekleri kullanıldı. Ki-67 belirteci kullanılarak, kolesteatomlu iki hasta grubu karşılaştırıldı.Bulgular: Kontrol grubuna göre, kolesteatomlu her ikigrupta hücresel proliferasyonda artış saptandı. Ki-67 boyanma yüzdeleri açısından, kolesteatomlu iki grup arasında anlamlı farklılık bulunmadı p>0.05 . Kolesteatomlugrupta Ki-67 değerleri kontrol grubuna göre anlamlı düzeyde yüksek bulunurken p

Anahtar Kelimeler

Kolesteatom, orta kulak, Ki-67 antijeni, immünohistokimya

Kaynakça

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