The comparison of the expansion of polyps according to the Ki-67 and computed tomography scores

Yıl 2009, Cilt: 19 Sayı: 4, 179 - 183, 18.08.2009

Sedat Aydın Arif Şanlı İlter Tezer Ümit Hardal Nagehan Özdemir Barışık

Öz

Objectives: The disease extention in nasal polyps was compared by using the mitotic activity rates and the computed tomography scores. Patients and Methods: This study was conducted on 19 nasal polyposis patients 8 males, 11 females; mean age 40.0±13.7 years; range 20 to 63 years . The preoperative computed tomography records of the patients were evaluated according to the Lund-Mackay grading system. The polyp tissues of the same patients were stained with the Ki-67 antigen for immunohistochemical evaluation. The correlation between the radiologic results and the Ki-67 values was compared by means of the Spearman’s correlation test. Results: The mean computed tomography score was observed as 14.3±4.7 range 7-24 . The mean Ki-67 score resulting from the immunohistochemical staining was calculated as 24.3±18.5 range %3.3-73.5 . A significant correlation was determined between the Ki-67 values and the computed tomography scores. “Spearman’s” correlation factor: 0.677; p<0.001 . Conclusion: As the mitotic activity rate of nasal polyps increases, both the volume of the polyps and the computed tomography scores increase as a result of the blockage of the sinus ostiums by the increased polyp volume.

Anahtar Kelimeler

Computed tomography, immunohistochemical assay, Ki-67, Nasal polyp

Polip yaygınlığının Ki-67 ve bilgisayarlı tomografi skorlarına göre karşılaştırılması

Öz

Amaç: Nazal poliplerde, hastalığın yaygınlığı mitotik aktivite oranları ve bilgisayarlı tomografi skorları kullanılarak karşılaştırıldı.Hastalar ve Yöntemler: Çalışmaya 19 nazal polipozis hastası 8 erkek, 11 kadın; ort. yaş 40.0±13.7 yıl; dağılım 20-63 yıl dahil edildi. Hastaların ameliyat öncesi bilgisayarlı tomografi kayıtları LundMackay evreleme sistemine göre değerlendirildi. Aynı hastaların polip dokuları immünohistokimyasal değerlendirme amacıyla Ki-67 antijeni ile boyandı. Radyolojik sonuçlarla Ki-67 boyanma yüzdeleri arasındaki ilişki Spearman korelasyon testi ile karşılaştırıldı.Bulgular: Hastaların bilgisayarlı tomografi skor ortalaması 14.3±4.7 olarak gözlendi. İmmünohistokimyasal boyama sonucunda elde edilen Ki-67 skor ortalaması 24.3±18.5 dağılım %3.373.5 olarak hesaplandı. Ki-67 değerleri ile bilgisayarlı tomografi skorları arasında istatistiksel olarak ileri düzeyde anlamlı ilişki saptandı Spearman korelasyon katsayısı: 0.677; p

Anahtar Kelimeler

Bilgisayarlı tomografi, immünohistokimyasal inceleme, Ki-67, nazal polip

Kaynakça

• Meço C. Nazal Poliplerin epidemiyolojisi. In: İleri F, editör. Nazal polipler. İstanbul: Deomed Medikal Yayıncılık; 2007. s. 1-10.
• Uzun KH. Nazal poliplerin anatomik orijini. In: İleri F, editör. Nazal polipler. İstanbul: Deomed Medikal Yayıncılık; 2007. s. 11-6.
• Uzun KH, Köybaşıoğlu F. Nazal poliplerin histopatolo- jisi. In: İleri F, editör. Nazal polipler. İstanbul: Deomed Medikal Yayıncılık; 2007. s. 17-24.
• Gerdes J, Schwab U, Lemke H, Stein H. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 1983;31:13-20.
• Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol 2000;182:311- 22.
• Yalçın S, Keleş E. Nazal polipoziste tanı ve ayırıcı tanı In: İleri F, editör. Nazal polipler. İstanbul: Deomed Medikal Yayıncılık; 2007. s. 41-56.
• Lund VJ, Mackay IS. Staging in rhinosinusitus. Rhinology 1993;31:183-4.
• Bujia J, Holly A, Sudhoff H, Antoli-Candela F, Tapia MG, Kastenbauer E. Identification of proliferating keratinocytes in middle ear cholesteatoma using the monoclonal antibody Ki-67. ORL J Otorhinolaryngol Relat Spec 1996;58:23-6.
• Huisman MA, De Heer E, Grote JJ. Cholesteatoma epithelium is characterized by increased expression of Ki-67, p53 and p21, with minimal apoptosis. Acta Otolaryngol 2003;123:377-82.
• Hsu MC, Shun CT, Liu CM. Increased epithelial cell proliferation in nasal polyps. J Formos Med Assoc 2002;101:227-9.
• Tezer İ, Şanlı A, Sarısoy B, Şen D, Barışık NÖ. Nazal poliplerdeki epitelyal hücre proliferasyonu ile fizik muayene bulgularının karşılaştırılması. KBB-Forum 2006;5:118-20.
• Özdek A, Meral İ, Bayız Ü, Çaydere M, Kılıç R, Samim E. Nazal polip ve antrokoanal polip dokularında epitel hücre proliferasyonu ve apoptozis inhibisyo- nu: İmmünhistokimyasal çalışma. PTT Hastanesi Tıp Dergisi 2002;24:143-7.
• Demirbağ RN, Batur Ş, Tarlacı A, Bilgili AM, Ersözlü T. Larinks skuamöz hücreli karsinomlarında Ki-67 eks- presyonu ve klinikopatolojik parametrelerle ilişkisi. Haseki Tıp Bülteni 2005;43:58-63.
• Liu M, Lawson G, Delos M, Jamart J, Ide C, Coche E, et al. Predictive value of the fraction of cancer cells immunolabeled for proliferating cell nuclear antigen or Ki67 in biopsies of head and neck carcino- mas to identify lymph node metastasis: comparison with clinical and radiologic examinations. Head Neck 2003;25:280-8.
• Bui MH, Visapaa H, Seligson D, Kim H, Han KR, Huang Y, et al. Prognostic value of carbonic anhydrase IX and KI67 as predictors of survival for renal clear cell carcinoma. J Urol 2004;171:2461-6.
• Sanli A, Tezer I, Paksoy M, Aydin S, Hardal U, Ozdemir NB. Evaluation of Ki-67 expression in recur- rent cases of cholesteatoma. [Article in Turkish] Kulak Burun Bogaz Ihtis Derg 2007;17:65-9.
• Larsen PL, Tos M, Kuijpers W, van der Beek JM. The early stages of polyp formation. Laryngoscope 1992;102:670-7.
• Petruson B, Hansson HA, Petruson K. Insulin-like growth factor I is a possible pathogenic mechanism in nasal polyps. Acta Otolaryngol 1988;106:156-60.
• Elovic A, Wong DT, Weller PF, Matossian K, Galli SJ. Expression of transforming growth factors-alpha and beta 1 messenger RNA and product by eosinophils in nasal polyps. J Allergy Clin Immunol 1994;93:864-9.
• Hassid S, Degaute MP, Dawance S, Rombaut K, Nagy N, Choufani G, et al. Determination of proliferative activity in nasal polyps. J Clin Pathol 1997;50:923-8.
• Coste A, Rateau JG, Bernaudin JF, Peynègre R, Escudier E. Nasal polyposis pathogenesis: a flow cytometric and immunohistochemical study of epithelial cell pro- liferation. Acta Otolaryngol 1996;116:755-61.
• Tryka E, Skomra D, Klatka J, Gieroba R, Olszanski W. Epithelial cell proliferation in nasal polyps. Otolaryngol Pol. 2005;59:523-6. [Abtract]
• Pawliczak R, Lewandowska-Polak A, Kowalski ML. Pathogenesis of nasal polyps: an update. Curr Allergy Asthma Rep 2005;5:463-71.
• Kawabori S, Kanai N, Tosho T. Proliferative activity of mast cells in allergic nasal mucosa. Clin Exp Allergy 1995;25:173-8.
• Reiss M, Neumann U, Reiss G. Importance of computer tomography in preoperative diagnostics of polyposis nasi. Wien Klin Wochenschr 2000;112:851-4. [Abtract]