Advanced oxidation protein products as an oxidative stress marker in allergic rhinitis

Yıl 2009, Cilt: 19 Sayı: 6, 279 - 284, 12.12.2009

Fadlullah Aksoy Hasan Demirhan Bayram Veyseller Yavuz Selim Yıldırım Orhan Özturan Filiz Basınoğlu

Öz

Objectives: This study was designed to compare the levels of dityrosine-containing protein cross-link products, also known as advanced oxidation protein products AOPP ; in patients with allergic rhinitis with those in healthy individuals considering the fact that AOPP has the potential to be a marker of oxidative stress specific to proteins in mammalian systems. Patients and Methods: This study was performed on 40 patients 18 males, 22 females; mean age 29 years; range 10 to 53 years with allergic rhinitis admitted to our clinic between December 2008 and January 2009 and on 40 healthy volunteers 16 males, 24 females; mean age 31 years; range 13 to 48 years . Skin prick test was performed to establish a diagnosis of allergic rhinitis in patients with major symptoms and allergic sensitization was also supported with serum IgE levels. Blood samples were obtained and examined in all patients to determine AOPP. Results: Serum AOPP levels were significantly higher in patients with allergic rhinitis 169.0±14.2 μmol/L compared to controls 43.9±3.5 μmol/L; p<0.001 . In addition, mean serum IgE levels of patients with allergic rhinitis 308.2±38.5 IU/ml were found to support the presence of allergic sensitization. Conclusion: Determining the levels of serum AOPP, a well-known marker of protein oxidation, appears to be a useful method in determining the role of oxidative stress in the etiopathogenesis of allergic rhinitis.

Anahtar Kelimeler

Advanced oxidation protein products, allergic rhinitis, oxidative stress, protein oxidation

Alerjik rinitte oksidatif stres göstergesi olarak ileri oksidasyon protein ürünleri

Öz

Amaç: Bu çalışma memeli sistemlerde proteinlere özgü oksidatif stres varlığının bir göstergesi olma potansiyeline dayanarak, alerjik rinitli hastaların plazmalarında ileri oksidasyon protein ürünleri İOPÜ olarak da bilinen ditirozin içerikli protein çapraz bağ ürünleri düzeyinin sağlıklı bireylerle karşılaştırılması amacıyla tasarlandı.Hastalar ve Yöntemler: Bu çalışma Aralık 2008 ile Ocak 2009 tarihleri arasında kliniğimize başvuran ve alerjik rinit tanısı konan 40 hasta 18 erkek, 22 kadın; ort. yaş 29 yıl; dağılım 10-53 yıl ve sağlıklı 40 gönüllü 16 erkek, 24 kadın; ort. yaş 31 yıl; dağılım 13-48 yıl ile gerçekleştirildi. Majör semptomları olan hastalara deri prik testi yapılarak alerjik rinit tanısı kondu ve alerjik sensitizasyon serum IgE düzeyleri ile de desteklendi. İleri oksidasyon protein ürünlerinin tespiti için tüm hastalardan kan örnekleri alındı ve incelendi.Bulgular: Serum İOPÜ düzeyleri alerjik rinitli hastalarda 169.0±14.2 μmol/L kontrol grubuna göre anlamlı şekilde daha yüksek bulundu 43.9±3.5 μmol/L; p

Anahtar Kelimeler

İleri oksidasyon protein ürünleri, alerjik rinit, oksidatif stres, protein oksidasyonu

Kaynakça

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