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Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis

Yıl 2010, Cilt: 20 Sayı: 3, 111 - 117, 30.06.2010

Öz

Objectives: The aim of this study was to compare the cytokine levels of subjects with nasal polyps NP and comorbid asthma and NP patients without asthma. Patients and Methods: Thirty patients with NP 15 asthmatic and 15 nonasthmatic were included in this prospective study. Nasal secretion samples were collected from the nasal cavities of all subjects. The levels of eleven cytokines TNF-a, TNF-b, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IFN-g were measured using flow cytometric method. Results: The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher in patients with NP and asthma compared with subjects with NP without asthma. IFN-g, IL-4, IL-6 and IL-10 levels were found significantly higher in allergic patients with NP and asthma compared with those without asthma. In nonallergic patients with NP and asthma, the concentrations of TNF-a, IL-5 and IL-6 were significantly higher than in nonallergic patients with NP without asthma. Conclusion: Our results showed that the presence of Th2 cytokines, especially IL-5 and IL-6 in patients with NP and asthma is a prominent feature that relates to increased eosinophilic inflammation. We also found significant influence of asthma and allergy on the cytokine profiles in nasal secretions of patients with NP.

Kaynakça

  • Fokkens W, Lund V, Mullol J. European Position Paper on Rhinosinusitis and Nasal Polyps group. European position paper on rhinosinusitis and nasal polyps 2007. Rhinol Suppl 2007;20:1-136.
  • Erbek SS, Yurtcu E, Erbek S, Atac FB, Sahin FI, Cakmak O. Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis. Arch Otolaryngol Head Neck Surg 2007;133:705-9.
  • Ramanathan M Jr, Lee WK, Spannhake EW, Lane AP. Th2 cytokines associated with chronic rhinosinusitis with polyps down-regulate the antimicrobial immune function of human sinonasal epithelial cells. Am J Rhinol 2008;22:115-21.
  • Král B, Krejsek J, Paráková Z, Kopecky´ O, Vokurková D, Derner V, et al. Some serum activity markers of airways inflammation in difficult-to-control asthma patients. Acta Medica (Hradec Kralove) 1997;40:61-70.
  • Jankowski R, Persoons M, Foliguet B, Coffinet L, Thomas C, Verient-Montaut B. Eosinophil count in nasal secretions of subjects with and without nasal symptoms. Rhinology 2000;38:23-32.
  • Riechelmann H, Deutschle T, Rozsasi A, Keck T, Polzehl D, Bürner H. Nasal biomarker profiles in acute and chronic rhinosinusitis. Clin Exp Allergy 2005;35:1186-91.
  • Riechelmann H, Deutschle T, Friemel E, Gross HJ, Bachem M. Biological markers in nasal secretions. Eur Respir J 2003;21:600-5.
  • Bousquet J. Global initiative for asthma (GINA) and its objectives. Clin Exp Allergy 2000;30 Suppl 1:2-5.
  • Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P, et al. Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. J Allergy Clin Immunol 2004;113:462-9.
  • Lildholdt T, Rundcrantz H, Bende M, Larsen K. Glucocorticoid treatment for nasal polyps. The use of topical budesonide powder, intramuscular betametha- sone, and surgical treatment. Arch Otolaryngol Head Neck Surg 1997;123:595-600.
  • Lund VJ, Mackay IS. Staging in rhinosinusitus. Rhinology 1993;31:183-4.
  • Watelet JB, Gevaert P, Holtappels G, Van Cauwenberge P, Bachert C. Collection of nasal secretions for immu- nological analysis. Eur Arch Otorhinolaryngol 2004; 261:242-6.
  • Bachert C, van Kempen M, Van Cauwenberge P. Regulation of proinflammatory cytokines in seasonal allergic rhinitis. Int Arch Allergy Immunol 1999; 118:375-9.
  • Ohkubo K, Ikeda M, Pawankar R, Gotoh M, Yagi T, Okuda M. Mechanisms of IL-6, IL-8, and GM-CSF release in nasal secretions of allergic patients after nasal challenge. Rhinology 1998;36:156-61.
  • Hens G, Hellings PW. The nose: gatekeeper and trigger of bronchial disease. Rhinology 2006;44:179-87.
  • Rinia AB, Kostamo K, Ebbens FA, van Drunen CM, Fokkens WJ. Nasal polyposis: a cellular-based approach to answering questions. Allergy 2007;62:348- 58.
  • Bachert C, Wagenmann M, Rudack C, Höpken K, Hillebrandt M, Wang D, et al. The role of cytokines in infectious sinusitis and nasal polyposis. Allergy 1998;53:2-13.
  • Hamilos DL, Leung DY, Wood R, Cunningham L, Bean DK, Yasruel Z, et al. Evidence for distinct cytokine expression in allergic versus nonallergic chronic sinus- itis. J Allergy Clin Immunol 1995;96:537-44.
  • Liu CM, Hong CY, Shun CT, Hsiao TY, Wang CC, Wang JS, et al. Inducible cyclooxygenase and interleukin 6 gene expressions in nasal polyp fibroblasts: possible implication in the pathogenesis of nasal polyposis. Arch Otolaryngol Head Neck Surg 2002;128:945-51.
  • Van Zele T, Gevaert P, Watelet JB, Claeys G, Holtappels G, Claeys C, et al. Staphylococcus aureus coloniza- tion and IgE antibody formation to enterotoxins is increased in nasal polyposis. J Allergy Clin Immunol 2004;114:981-3.
  • Hellings PW, Hens G, Meyts I, Bullens D, Vanoirbeek J, Gevaert P, et al. Aggravation of bronchial eosino- philia in mice by nasal and bronchial exposure to Staphylococcus aureus enterotoxin B. Clin Exp Allergy 2006;36:1063-71.
  • Xu G, Xia JH, Zhou H, Yu CZ, Zhang Y, Zuo KJ, et al. Interleukin-6 is essential for Staphylococcal exotoxin B-induced T regulatory cell insufficiency in nasal pol- yps. Clin Exp Allergy 2009;39:829-37.
  • Dellacono FR, Eisma R, Lafreniere D, Leonard G, Kreutzer D. Interferon gamma expression in human nasal polyps. Laryngoscope 1997;107:626-30.
  • Ohori J, Ushikai M, Sun D, Nishimoto K, Sagara Y, Fukuiwa T, et al. TNF-alpha upregulates VCAM-1 and NF-kappaB in fibroblasts from nasal polyps. Auris Nasus Larynx 2007;34:177-83.

Astımlı ve astımlı olmayan nazal polipozisli hastaların nazal sekresyonlarındaki sitokin seviyeleri

Yıl 2010, Cilt: 20 Sayı: 3, 111 - 117, 30.06.2010

Öz

Amaç: Bu çalışmada nazal polip NP ’leri ve hastalığa eşlik eden astımı olan ve olmayan NP’li hastaların sitokin seviyeleri karşılaştırıldı.Hastalar ve Yöntemler: Bu ileriye yönelik çalışmaya, nazal polipleri olan 30 hasta 15 astımlı ve 15 astımlı olmayan dahil edildi. Tüm olguların nazal kavitelerinden nazal sekresyon örnekleri biriktirildi. On bir sitokinin TNF-a, TNF-b, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 ve IFN-g seviyeleri akım sitometri yöntemi ile ölçüldü.Bulgular: Th2 sitokinlerinin IL-5, IL-6 ve IL-10 konsantrasyonları astımı olan NP’li hastalarda, astımı olmayan NP’li hastalara göre anlamlı derecede yüksek bulundu. Nazal polipli ve astımı olan alerjik hastalarda, IFN-g, IL-4, IL-6 ve IL-10 seviyeleri, astımı olmayanlara göre anlamlı derecede yüksek bulundu. Alerjik olmayan, nazal polipleri ve astımı olan hastalarda TNF-a, IL-5 ve IL-6 konsantrasyonları, alerjisi ve astımı olmayan NP’li hastalara göre oldukça yüksek bulundu.Sonuç: Bizim sonuçlarımıza göre, Th2 sitokinlerinin, özellikle de IL-5 ve IL-6’nın varlığı, NP ve astımı olan hastalarda eozinofilik inflamasyonun artışıyla ilişkili belirgin bir özelliktir. Biz aynı zamanda astım ve alerjinin, NP’li hastaların nazal sekresyonlarındaki sitokin profiline önemli etkisinin olduğunu saptadık

Kaynakça

  • Fokkens W, Lund V, Mullol J. European Position Paper on Rhinosinusitis and Nasal Polyps group. European position paper on rhinosinusitis and nasal polyps 2007. Rhinol Suppl 2007;20:1-136.
  • Erbek SS, Yurtcu E, Erbek S, Atac FB, Sahin FI, Cakmak O. Proinflammatory cytokine single nucleotide polymorphisms in nasal polyposis. Arch Otolaryngol Head Neck Surg 2007;133:705-9.
  • Ramanathan M Jr, Lee WK, Spannhake EW, Lane AP. Th2 cytokines associated with chronic rhinosinusitis with polyps down-regulate the antimicrobial immune function of human sinonasal epithelial cells. Am J Rhinol 2008;22:115-21.
  • Král B, Krejsek J, Paráková Z, Kopecky´ O, Vokurková D, Derner V, et al. Some serum activity markers of airways inflammation in difficult-to-control asthma patients. Acta Medica (Hradec Kralove) 1997;40:61-70.
  • Jankowski R, Persoons M, Foliguet B, Coffinet L, Thomas C, Verient-Montaut B. Eosinophil count in nasal secretions of subjects with and without nasal symptoms. Rhinology 2000;38:23-32.
  • Riechelmann H, Deutschle T, Rozsasi A, Keck T, Polzehl D, Bürner H. Nasal biomarker profiles in acute and chronic rhinosinusitis. Clin Exp Allergy 2005;35:1186-91.
  • Riechelmann H, Deutschle T, Friemel E, Gross HJ, Bachem M. Biological markers in nasal secretions. Eur Respir J 2003;21:600-5.
  • Bousquet J. Global initiative for asthma (GINA) and its objectives. Clin Exp Allergy 2000;30 Suppl 1:2-5.
  • Tsicopoulos A, Shimbara A, de Nadai P, Aldewachi O, Lamblin C, Lassalle P, et al. Involvement of IL-9 in the bronchial phenotype of patients with nasal polyposis. J Allergy Clin Immunol 2004;113:462-9.
  • Lildholdt T, Rundcrantz H, Bende M, Larsen K. Glucocorticoid treatment for nasal polyps. The use of topical budesonide powder, intramuscular betametha- sone, and surgical treatment. Arch Otolaryngol Head Neck Surg 1997;123:595-600.
  • Lund VJ, Mackay IS. Staging in rhinosinusitus. Rhinology 1993;31:183-4.
  • Watelet JB, Gevaert P, Holtappels G, Van Cauwenberge P, Bachert C. Collection of nasal secretions for immu- nological analysis. Eur Arch Otorhinolaryngol 2004; 261:242-6.
  • Bachert C, van Kempen M, Van Cauwenberge P. Regulation of proinflammatory cytokines in seasonal allergic rhinitis. Int Arch Allergy Immunol 1999; 118:375-9.
  • Ohkubo K, Ikeda M, Pawankar R, Gotoh M, Yagi T, Okuda M. Mechanisms of IL-6, IL-8, and GM-CSF release in nasal secretions of allergic patients after nasal challenge. Rhinology 1998;36:156-61.
  • Hens G, Hellings PW. The nose: gatekeeper and trigger of bronchial disease. Rhinology 2006;44:179-87.
  • Rinia AB, Kostamo K, Ebbens FA, van Drunen CM, Fokkens WJ. Nasal polyposis: a cellular-based approach to answering questions. Allergy 2007;62:348- 58.
  • Bachert C, Wagenmann M, Rudack C, Höpken K, Hillebrandt M, Wang D, et al. The role of cytokines in infectious sinusitis and nasal polyposis. Allergy 1998;53:2-13.
  • Hamilos DL, Leung DY, Wood R, Cunningham L, Bean DK, Yasruel Z, et al. Evidence for distinct cytokine expression in allergic versus nonallergic chronic sinus- itis. J Allergy Clin Immunol 1995;96:537-44.
  • Liu CM, Hong CY, Shun CT, Hsiao TY, Wang CC, Wang JS, et al. Inducible cyclooxygenase and interleukin 6 gene expressions in nasal polyp fibroblasts: possible implication in the pathogenesis of nasal polyposis. Arch Otolaryngol Head Neck Surg 2002;128:945-51.
  • Van Zele T, Gevaert P, Watelet JB, Claeys G, Holtappels G, Claeys C, et al. Staphylococcus aureus coloniza- tion and IgE antibody formation to enterotoxins is increased in nasal polyposis. J Allergy Clin Immunol 2004;114:981-3.
  • Hellings PW, Hens G, Meyts I, Bullens D, Vanoirbeek J, Gevaert P, et al. Aggravation of bronchial eosino- philia in mice by nasal and bronchial exposure to Staphylococcus aureus enterotoxin B. Clin Exp Allergy 2006;36:1063-71.
  • Xu G, Xia JH, Zhou H, Yu CZ, Zhang Y, Zuo KJ, et al. Interleukin-6 is essential for Staphylococcal exotoxin B-induced T regulatory cell insufficiency in nasal pol- yps. Clin Exp Allergy 2009;39:829-37.
  • Dellacono FR, Eisma R, Lafreniere D, Leonard G, Kreutzer D. Interferon gamma expression in human nasal polyps. Laryngoscope 1997;107:626-30.
  • Ohori J, Ushikai M, Sun D, Nishimoto K, Sagara Y, Fukuiwa T, et al. TNF-alpha upregulates VCAM-1 and NF-kappaB in fibroblasts from nasal polyps. Auris Nasus Larynx 2007;34:177-83.
Toplam 24 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Aleksandar Perić Bu kişi benim

Danilo Vojvodić Bu kişi benim

Vesna Radulović Bu kişi benim

Olivera Miljanović Bu kişi benim

Yayımlanma Tarihi 30 Haziran 2010
Yayımlandığı Sayı Yıl 2010 Cilt: 20 Sayı: 3

Kaynak Göster

APA Perić, A., Vojvodić, D., Radulović, V., Miljanović, O. (2010). Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis. The Turkish Journal of Ear Nose and Throat, 20(3), 111-117.
AMA Perić A, Vojvodić D, Radulović V, Miljanović O. Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis. Tr-ENT. Haziran 2010;20(3):111-117.
Chicago Perić, Aleksandar, Danilo Vojvodić, Vesna Radulović, ve Olivera Miljanović. “Cytokine Levels in Nasal Secretions in Asthmatic and Nonasthmatic Patients With Nasal Polyposis”. The Turkish Journal of Ear Nose and Throat 20, sy. 3 (Haziran 2010): 111-17.
EndNote Perić A, Vojvodić D, Radulović V, Miljanović O (01 Haziran 2010) Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis. The Turkish Journal of Ear Nose and Throat 20 3 111–117.
IEEE A. Perić, D. Vojvodić, V. Radulović, ve O. Miljanović, “Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis”, Tr-ENT, c. 20, sy. 3, ss. 111–117, 2010.
ISNAD Perić, Aleksandar vd. “Cytokine Levels in Nasal Secretions in Asthmatic and Nonasthmatic Patients With Nasal Polyposis”. The Turkish Journal of Ear Nose and Throat 20/3 (Haziran 2010), 111-117.
JAMA Perić A, Vojvodić D, Radulović V, Miljanović O. Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis. Tr-ENT. 2010;20:111–117.
MLA Perić, Aleksandar vd. “Cytokine Levels in Nasal Secretions in Asthmatic and Nonasthmatic Patients With Nasal Polyposis”. The Turkish Journal of Ear Nose and Throat, c. 20, sy. 3, 2010, ss. 111-7.
Vancouver Perić A, Vojvodić D, Radulović V, Miljanović O. Cytokine levels in nasal secretions in asthmatic and nonasthmatic patients with nasal polyposis. Tr-ENT. 2010;20(3):111-7.