An evaluation of the radioprotective effect of vitamin E on the salivary glands of radioactive iodine in rats

Yıl 2014, Cilt: 24 Sayı: 1, 21 - 29, 15.02.2014

Filiz Aydoğan Hasan İkbal Atılgan Gökhan Koca Nihat Yumuşak Emine Aydın Murat Sadıç Meliha Korkmaz Salih Tuncal Ethem Erdal Samim

Öz

Objectives: This study aims to examine the acute effect of vitamin E on salivary gland of radioactive iodine RAI; 131I -induced rats and to evaluate whether vitamin E have a radioprotective effect. Materials and Methods: Sixteen Wistar albino rats were used in the study. The rats were divided into two groups. The first group was administered 131I orally and 1 ml physiological saline. The second group was administered 131I and 1 ml vitamin E intraperitoneally. Vitamin E was started two days before the RAI therapy and continued for seven days. On the eighth day, salivary glands were removed and evaluated histopathologically. Results: There was no statistically significant difference between the groups. The changes in the interstitial space of all glands and in acinar epithelial cells of parotid and sublingual glands were observed in a lower number of the rats of vitamin E group, compared to the controls. Except panacinar inflammation, histopathological changes in acinar epithelial cells of the submandibular gland were noticed in a lower number of the rats of vitamin E group. Conclusion: Considering the changes in the interstitial space and acinar epithelial cells in a lower number of the rats of vitamin E group, we conclude that vitamin E may have protective effects for interstitial space of all glands and acinar epithelial cells of the parotid and sublingual glands during the acute period.

Anahtar Kelimeler

131I treatment, antioxidant, histopathology, rat, salivary gland, vitamin E

Radyoaktif iyot verilen sıçanlarda tükürük bezleri üzerinde E vitaminin radyasyondan koruyucu etkisinin değerlendirilmesi

Öz

Amaç: Bu çalışmada radyoaktif iyot RAİ; 131I uygulanmış sıçanların tükürük bezlerinde E vitamininin akut etkisi incelendi ve radyasyondan koruyucu etkiye sahip olup olmadığı değerlendirildi.Gereç ve Yöntem: Çalışmada 16 Wistar albino sıçan kullanıldı. Sıçanlar iki gruba ayrıldı. Birinci grupta 131I oral yolla ve 1 ml serum fizyolojik uygulandı. İkinci grupta 131I ve 1 ml E vitamini intraperitoneal olarak verildi. E vitaminine radyoaktif iyot tedavisinden iki gün önce başlanılarak yedi gün süreyle devam edildi. Sekizinci günde tükürük bezleri çıkarıldı ve histopatolojik olarak incelendi.Bulgular: Gruplar arasında istatistiksel olarak anlamlı fark yoktu. Tüm bezlerin interstisyel alanındaki değişiklikler, parotis ve sublingual bezlerin asiner epitelyal hücrelerindeki değişiklikler kontrol grubuna kıyasla, E vitamini grubunda daha az sayıda sıçanda izlendi. Submandibüler bezin asiner epitelyal hücrelerinde panasiner enflamasyon dışındaki histopatolojik değişiklikler, E vitamini grubunda daha az sayıda sıçanda tespit edildi.Sonuç: İnterstisyel alan ve asiner epitelyal hücrelerde E vitamini grubunda daha az sayıdaki sıçanda değişiklikler gözlendiği göz önüne alındığında, tüm bezlerin interstisyel alanı, parotis ve sublingual bezlerin asiner epitelyal hücreleri için E vitamininin akut dönemde koruyucu etkilerinin olabileceği sonucuna varıldı

Anahtar Kelimeler

I tedavisi, antioksidan, histopatoloji, sıçan, tükürük bezi, E vitamini

Kaynakça

• Chow SM. Side effects of high-dose radioactive iodine for ablation or treatment of differentiated thyroid carcinoma. JHK Coll Radiol 2005;8:127-35.
• Grewal RK, Larson SM, Pentlow CE, Pentlow KS, Gonen M, Qualey R, et al. Salivary gland side effects commonly develop several weeks after initial radioactive iodine ablation. J Nucl Med 2009;50:1605-10.
• Koca G, Gültekin SS, Han U, Kuru S, Demirel K, Korkmaz M. The efficacy of montelukast as a protective agent against 131 I-induced salivary gland damage in rats: scintigraphic and histopathological findings. Nucl Med Commun 2013;34:507-17.
• Jeong SY, Lee J. Radiation sialadenitis induced by high-dose radioactive iodine therapy. Nucl Med Mol Imaging 2010;44:102-9.
• Ma C, Xie J, Jiang Z, Wang G, Zuo S. Does amifostine have radioprotective effects on salivary glands in high- dose radioactive iodine-treated differentiated thyroid cancer. Eur J Nucl Med Mol Imaging 2010;37:1778-85.
• Bhartiya US, Raut YS, Joseph LJ, Hawaldar RW, Rao BS. Evaluation of the radioprotective effect of turmeric extract and vitamin E in mice exposed to therapeutic dose of radioiodine. Indian J Clin Biochem 2008;23:382-6.
• Aydogan F, Aydın E, Tastan E, Arslan N, Senes M, Ünlü İ, Kavuzlu A. Is there a relationship between serum levels of vitamin A, vitamin E, copper and zinc and otitis media with effusion in children? Indian J Otolaryngol Head Neck Surg 2013;65:594-7.
• Weiss JF, Landauer MR. Protection against ionizing radiation by antioxidant nutrients and phytochemicals. Toxicology 2003;189:1-20.
• Fallahi B, Beiki D, Abedi SM, Saghari M, Fard- Esfahani A, Akhzari F, et al. Does vitamin E protect salivary glands from I-131 radiation damage in patients with thyroid cancer? Nucl Med Commun 2013;34:777-86.
• Vayre L, Sabourin JC, Caillou B, Ducreux M, Schlumberger M, Bidart JM. Immunohistochemical analysis of Na+/I- symporter distribution in human extra-thyroidal tissues. Eur J Endocrinol 1999;141:382-6.
• Spitzweg C, Joba W, Schriever K, Goellner JR, Morris JC, Heufelder AE. Analysis of human sodium iodide symporter immunoreactivity in human exocrine glands. J Clin Endocrinol Metab 1999;84:4178-84.
• Almeida JP, Sanabria AE, Lima EN, Kowalski LP. Late side effects of radioactive iodine on salivary gland function in patients with thyroid cancer. Head Neck 2011;33:686-90. teşekkür ederler.
• Konings AW, Coppes RP, Vissink A. On the mechanism of salivary gland radiosensitivity. Int J Radiat Oncol Biol Phys 2005;62:1187-94.
• Bianchi G, Solaroli E, Zaccheroni V, Grossi G, Bargossi AM, Melchionda N, et al. Oxidative stress and anti- oxidant metabolites in patients with hyperthyroidism: effect of treatment. Horm Metab Res 1999;31:620-4.
• Lee SL. Complications of radioactive iodine treatment of thyroid carcinoma. J Natl Compr Canc Netw 2010;8:1277-86.
• Solans R, Bosch JA, Galofré P, Porta F, Roselló J, Selva-O'Callagan A, et al. Salivary and lacrimal gland dysfunction (sicca syndrome) after radioiodine therapy. J Nucl Med 2001;42:738-43.
• Ramos FM, Pontual ML, de Almeida SM, Bóscolo FN, Tabchoury CP, Novaes PD. Evaluation of radioprotective effect of vitamin E in salivary dysfunction in irradiated rats. Arch Oral Biol 2006;51:96-101.