Investigation of Ototoxicity of Intratympanic 5-fluorouracil in Rats

Year 2022, Volume: 32 Issue: 4, 115 - 120, 24.01.2023

Meltem Demirdağ Çevikkan , Asım Aslan , Elgin Türköz Uluer , Damla Demirkaya Tas

https://doi.org/10.26650/Tr-ENT.2022.1216068

Abstract

Objective: Although 5-fluorouracil is beneficial in treating cholesteatoma in people, it is unknown if this drug harms the inner ear. We are planning to contribute to the literature by investigating the effects of the intratympanic administration of 5-fluorouracil solution form to the middle ear on the inner ear in rats. Materials and Methods: The study was conducted on 11 Wistar albino male rats: a positive control group was treated with amikacin, a study group was treated with 5-FU, and a negative control group received no treatment. One week after intratympanic drug administration, the rats were sacrificed, and ototoxicity was histopathologically examined by light microscopy and TUNEL (Terminal deoxynucleotidyl transferasemediated dUTP Nick End Labeling) method. Results: There was a significant difference between the two groups treated with amikacin and 5-fluorouracil in terms of apoptosis (p<0.05). The difference in stria vascularis thicknesses was significant between the amikacin group and the 5-fluorouracil group and the negative control group (p<0.05). Conclusion: The intratympanic administration of 5-fluorouracil to rats did not have any ototoxic effects, according to the results of the histological analysis that looked at apoptosis.

Keywords

5-fluorouracil, ototoxicity, intratympanic injection, animal model

Supporting Institution

Scientific Research Projects Coordination Unit of Manisa Celal Bayar University

Project Number

109-2009

Thanks

The authors would like to thank the people that work at experimental animals laboratory.

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