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Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study

Yıl 2024, Cilt: 34 Sayı: 2, 37 - 41, 10.06.2024
https://doi.org/10.26650/Tr-ENT.2024.1426956

Öz

Objective: Gentamicin (GM) is a potent antibiotic but is known to cause irreversible ototoxic effects. Nigella sativa oil (NSO) can offer protective prophylaxis against ototoxicity when administered prior to GM treatment. This study aims to assess the impact of delayed NSO administration on GM-induced ototoxicity using the auditory brainstem response (ABR) test.

Material and Methods: Adult male Sprague-Dawley rats were randomly divided into three groups, each consisting of six animals. All groups received intraperitoneal (i.p.) GM (120 mg/kg i.p.) for a duration of 10 days. The first two groups were given 0.3 ml/kg/day NSO for five days, while the third group received 0.9% saline for the same duration. Group 1 involves the early administration of NSO (NSOE) 10 days after starting GM, Group 2 involves the late administration of NSO (NSOL) 25 days after starting GM, and Group 3 is the saline (control) group. Hearing thresholds were recorded for both ears prior to treatment initiation and again 30 days after the start of treatment.

Results: Two rats expired and were excluded from the study. A total of 64 ABR test results were obtained. No difference was found between the pre- and post-treatment ABR values in Groups 1 and 2. However, a significant increase in ABR thresholds was observed only in Group 3. A significant difference was found in post-treatment hearing thresholds (p<0.001) between Group 3 and the other two groups.

Conclusion: The results suggest the delayed administration of NSO within a period of 30 days, whether earlier or later, to be able to effectively reverse the GM-induced ototoxic effects.

Kaynakça

  • 1. Unal OF, Ghoreishi SM, Atas A, Akyürek N, Akyol G, Gürsel B. Prevention of Gentamicin-induced ototoxicity by trimetazidine in animal model. Int J Pediatr Otorhinolaryngol 2005;69(2):193-9. google scholar
  • 2. Xie J, Talaska AE, Schacht J. New developments in aminoglycoside therapy and ototoxicity. Hear Res 2011;281(1-2):28-37. google scholar
  • 3. Edizer DT, Yiğit O, Çınar Z, Gül M, Kara E, Yiğitcan B, et al. Protective role of intratympanic nigella sativa oil against gentamicin-induced hearing loss. Int J Pediatr Otorhinolaryngol 2017;97:83-8. google scholar
  • 4. Wu WJ, Sha SH, McLaren JD, Kawamoto K, Raphael Y, Schacht J. Aminoglycoside ototoxicity in adult CBA, C57BL and BALB mice and the Sprague-Dawley rat. Hear Res 2001;158(1-2):165-78. google scholar
  • 5. Alsuhaibani AAM. Effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats. Ital J Food Saf 2018;7(2):7242. google scholar
  • 6. Arslan S, Gelir E, Armutcu F, Coskun Ö, Gurel A, Sayan H, et al. The protective effect of thymoquinone on ethanol-induced acute gastric damage in the rats. Nutrit Res 2005;25(7):673-7. google scholar
  • 7. Kökten N, Eğilmez OK, Ekici AID, Kalcıoğlu MT, Tekin M, Yeşilada E. The effect of nigella sativa oil on prevention of myringosclerosis in a guinea pig model. Int J Pediatr Otorhinolaryngol 2016;88:52-7. google scholar
  • 8. Sagit M, Korkmaz F, Gürgen SG, Kaya M, Akçadağ A. The protective role of thymoquinone in the prevention of gentamicin ototoxicity. Am J of HN Surg 2014;35:603-9. google scholar
  • 9. Tuna B, Tüzemen G. Protective effects of nigella sativa oil against gentamicin-induced ototoxicity in rats: a dose-ranging study. Int J Pediatr Otorhinolaryngol 2023;164:111405. google scholar
  • 10. Tanoshima R, Khan A, Biala AK. Analyses of Adverse Drug Reactions Nationwide Active Surveillance Network: Canadian Pharmacogenomics Network for Drug Safety Database. J Clin Pharmacol 2019;59(3):356-63. google scholar
  • 11. Rajput K, Edwards L, Brock P, Abiodun A, Simpkin P, Al-Malky G. Ototoxicity-induced hearing loss and quality of life in survivors of pediatric cancer. Int J Pediatr Otorhinolaryngol, 2020;138:110401. google scholar
  • 12. Lanvers-Kaminsky C, Zehnhoff-Dinnesen AA, Parfitt R, Ciarimboli G. Drug-induced ototoxicity: Mechanisms, Pharmacogenetics, and protective strategies. Clin Pharmacol Ther 2017;101(4):491-500. google scholar
  • 13. Rizk HG, Lee JA, Liu YF, Endriukaitis L, Isaac JL, Bullington WM. Drug-Induced Ototoxicity: A Comprehensive Review and Reference Guide Bullington. Pharmacotherapy 2020;40(12):1265-75. google scholar
  • 14. Hamed SA. The auditory and vestibular toxicities induced by antiepileptic drugs. Expert Opinion on Drug Safety 2017;16(11):1281-94. google scholar
  • 15. Meyers RM. Ototoxic effects of gentamicin. Arch Otolaryngol 1970;92(2):160-2. google scholar
  • 16. Lesniak W, Pecoraro VL, Schacht J. Ternary complexes of gentamicin with iron and lipid catalyze formation of reactive oxygen species. Chem Res Toxicol 2005;18(2):357-64. google scholar
  • 17. Prezant TR, Agapian JV, Bohlman MC, Bu X, Oztas S, Qiu WQ, et al. Mitochondrial ribosomal RNA mutation associated with both antibiotic-induced and non-syndromic deafness. Nat Genet 1993;4(3):289-94. google scholar
  • 18. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17(4):299-305. google scholar
  • 19. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14(5):323-8. google scholar
  • 20. Kanter M, Coskun O, Uysal H. The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone, on ethanol-induced gastric mucosal damage. Arch Toxicol 2006;80(4):217-7. google scholar
  • 21. Kooti W, Hasanzadeh-Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chin J Nat Med 2016;14(10):732-45. google scholar
  • 22. Jaswal A, Sharma M, Raghuvanshi S, Sharma S, Reshi MS, Uthra C, et al. Therapeutic Efficacy of Nigella Sativa Linn. against Antituberculosis Drug-Induced Hepatic Injury in Wistar Rats. J Environ Pathol Toxicol Oncol 2016;35(1):59-71. google scholar
  • 23. Hassana SS, Razzaquec A, Ahmad Z, Pazdernik V, Amin SN. Does post-treatment thymoquinone reverse high-dose, atorvastatin-induced hepatic oxidative injury in rats? Can J Physiol Pharmacol 2018;96(1):51-9. google scholar
Yıl 2024, Cilt: 34 Sayı: 2, 37 - 41, 10.06.2024
https://doi.org/10.26650/Tr-ENT.2024.1426956

Öz

Kaynakça

  • 1. Unal OF, Ghoreishi SM, Atas A, Akyürek N, Akyol G, Gürsel B. Prevention of Gentamicin-induced ototoxicity by trimetazidine in animal model. Int J Pediatr Otorhinolaryngol 2005;69(2):193-9. google scholar
  • 2. Xie J, Talaska AE, Schacht J. New developments in aminoglycoside therapy and ototoxicity. Hear Res 2011;281(1-2):28-37. google scholar
  • 3. Edizer DT, Yiğit O, Çınar Z, Gül M, Kara E, Yiğitcan B, et al. Protective role of intratympanic nigella sativa oil against gentamicin-induced hearing loss. Int J Pediatr Otorhinolaryngol 2017;97:83-8. google scholar
  • 4. Wu WJ, Sha SH, McLaren JD, Kawamoto K, Raphael Y, Schacht J. Aminoglycoside ototoxicity in adult CBA, C57BL and BALB mice and the Sprague-Dawley rat. Hear Res 2001;158(1-2):165-78. google scholar
  • 5. Alsuhaibani AAM. Effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats. Ital J Food Saf 2018;7(2):7242. google scholar
  • 6. Arslan S, Gelir E, Armutcu F, Coskun Ö, Gurel A, Sayan H, et al. The protective effect of thymoquinone on ethanol-induced acute gastric damage in the rats. Nutrit Res 2005;25(7):673-7. google scholar
  • 7. Kökten N, Eğilmez OK, Ekici AID, Kalcıoğlu MT, Tekin M, Yeşilada E. The effect of nigella sativa oil on prevention of myringosclerosis in a guinea pig model. Int J Pediatr Otorhinolaryngol 2016;88:52-7. google scholar
  • 8. Sagit M, Korkmaz F, Gürgen SG, Kaya M, Akçadağ A. The protective role of thymoquinone in the prevention of gentamicin ototoxicity. Am J of HN Surg 2014;35:603-9. google scholar
  • 9. Tuna B, Tüzemen G. Protective effects of nigella sativa oil against gentamicin-induced ototoxicity in rats: a dose-ranging study. Int J Pediatr Otorhinolaryngol 2023;164:111405. google scholar
  • 10. Tanoshima R, Khan A, Biala AK. Analyses of Adverse Drug Reactions Nationwide Active Surveillance Network: Canadian Pharmacogenomics Network for Drug Safety Database. J Clin Pharmacol 2019;59(3):356-63. google scholar
  • 11. Rajput K, Edwards L, Brock P, Abiodun A, Simpkin P, Al-Malky G. Ototoxicity-induced hearing loss and quality of life in survivors of pediatric cancer. Int J Pediatr Otorhinolaryngol, 2020;138:110401. google scholar
  • 12. Lanvers-Kaminsky C, Zehnhoff-Dinnesen AA, Parfitt R, Ciarimboli G. Drug-induced ototoxicity: Mechanisms, Pharmacogenetics, and protective strategies. Clin Pharmacol Ther 2017;101(4):491-500. google scholar
  • 13. Rizk HG, Lee JA, Liu YF, Endriukaitis L, Isaac JL, Bullington WM. Drug-Induced Ototoxicity: A Comprehensive Review and Reference Guide Bullington. Pharmacotherapy 2020;40(12):1265-75. google scholar
  • 14. Hamed SA. The auditory and vestibular toxicities induced by antiepileptic drugs. Expert Opinion on Drug Safety 2017;16(11):1281-94. google scholar
  • 15. Meyers RM. Ototoxic effects of gentamicin. Arch Otolaryngol 1970;92(2):160-2. google scholar
  • 16. Lesniak W, Pecoraro VL, Schacht J. Ternary complexes of gentamicin with iron and lipid catalyze formation of reactive oxygen species. Chem Res Toxicol 2005;18(2):357-64. google scholar
  • 17. Prezant TR, Agapian JV, Bohlman MC, Bu X, Oztas S, Qiu WQ, et al. Mitochondrial ribosomal RNA mutation associated with both antibiotic-induced and non-syndromic deafness. Nat Genet 1993;4(3):289-94. google scholar
  • 18. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res 2003;17(4):299-305. google scholar
  • 19. Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14(5):323-8. google scholar
  • 20. Kanter M, Coskun O, Uysal H. The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone, on ethanol-induced gastric mucosal damage. Arch Toxicol 2006;80(4):217-7. google scholar
  • 21. Kooti W, Hasanzadeh-Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary-Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chin J Nat Med 2016;14(10):732-45. google scholar
  • 22. Jaswal A, Sharma M, Raghuvanshi S, Sharma S, Reshi MS, Uthra C, et al. Therapeutic Efficacy of Nigella Sativa Linn. against Antituberculosis Drug-Induced Hepatic Injury in Wistar Rats. J Environ Pathol Toxicol Oncol 2016;35(1):59-71. google scholar
  • 23. Hassana SS, Razzaquec A, Ahmad Z, Pazdernik V, Amin SN. Does post-treatment thymoquinone reverse high-dose, atorvastatin-induced hepatic oxidative injury in rats? Can J Physiol Pharmacol 2018;96(1):51-9. google scholar
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Kulak Burun Boğaz
Bölüm Araştırma Makaleleri
Yazarlar

Bilge Tuna 0000-0001-6890-133X

Gökhan Tüzemen 0000-0001-9358-2019

Yayımlanma Tarihi 10 Haziran 2024
Gönderilme Tarihi 28 Ocak 2024
Kabul Tarihi 29 Nisan 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 34 Sayı: 2

Kaynak Göster

APA Tuna, B., & Tüzemen, G. (2024). Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study. The Turkish Journal of Ear Nose and Throat, 34(2), 37-41. https://doi.org/10.26650/Tr-ENT.2024.1426956
AMA Tuna B, Tüzemen G. Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study. Tr-ENT. Haziran 2024;34(2):37-41. doi:10.26650/Tr-ENT.2024.1426956
Chicago Tuna, Bilge, ve Gökhan Tüzemen. “Is Gentamicin-Induced Ototoxicity Reversible With Delayed Administration of Nigella Sativa Oil? An Experimental Study”. The Turkish Journal of Ear Nose and Throat 34, sy. 2 (Haziran 2024): 37-41. https://doi.org/10.26650/Tr-ENT.2024.1426956.
EndNote Tuna B, Tüzemen G (01 Haziran 2024) Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study. The Turkish Journal of Ear Nose and Throat 34 2 37–41.
IEEE B. Tuna ve G. Tüzemen, “Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study”, Tr-ENT, c. 34, sy. 2, ss. 37–41, 2024, doi: 10.26650/Tr-ENT.2024.1426956.
ISNAD Tuna, Bilge - Tüzemen, Gökhan. “Is Gentamicin-Induced Ototoxicity Reversible With Delayed Administration of Nigella Sativa Oil? An Experimental Study”. The Turkish Journal of Ear Nose and Throat 34/2 (Haziran 2024), 37-41. https://doi.org/10.26650/Tr-ENT.2024.1426956.
JAMA Tuna B, Tüzemen G. Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study. Tr-ENT. 2024;34:37–41.
MLA Tuna, Bilge ve Gökhan Tüzemen. “Is Gentamicin-Induced Ototoxicity Reversible With Delayed Administration of Nigella Sativa Oil? An Experimental Study”. The Turkish Journal of Ear Nose and Throat, c. 34, sy. 2, 2024, ss. 37-41, doi:10.26650/Tr-ENT.2024.1426956.
Vancouver Tuna B, Tüzemen G. Is Gentamicin-Induced Ototoxicity Reversible with Delayed Administration of Nigella Sativa Oil? An Experimental Study. Tr-ENT. 2024;34(2):37-41.