Comparative Analysis of Using Isozyme and Issr-Pcr Markers for Population Differentiation of Cyprinid Fish

Yıl 2013, , - , 01.02.2013

Oxana Nikolaevna Zhigileva Oxana Georgievna Baranova Viktor Valentinovich Pozhidaev Ivan Sergeevich Brol Tatyana Ivanovna Moiseenko

https://doi.org/10.4194/1303-2712-v13_1_19

Öz

The article presents data on genetic variability and differentiation of three species of cyprinid fish - roach Rutilus rutilus, ide Leuciscus idus and dace Leuciscus leuciscus baikalensis in the rivers of Siberia, Russia. From the three species studied ide has the lowest indices of genetic variability and more pronounced population differentiation. About 40% of isozyme loci are polymorphic in this species and the same percent - for nuclear DNA inter-simple sequence repeat (ISSR) markers; interpopulation component accounts for 62% of genetic variability. In roach indicators of genetic variability are higher, especially for DNA markers (67%), and interpopulation differentiation is less pronounced (FST = 0.550; GST = 0.214). Dace has the most significant genetic diversity and low differentiation of populations. 48% of the isozyme loci and 83% of multilocus DNA markers are polymorphic in this species, the interpopulation component accounts for 27% and 19% for two types of markers, respectively. In comparison with allozymes, ISSR markers provide higher estimates of genetic distances while making intrageneric comparisons and similar or somewhat lower values - while making a comparison of different groups. Isozyme markers provide more precise geographical differences of population groups of fish.

Anahtar Kelimeler

Cyprinid fish, Siberia rivers, Genetic differentiation, Allozymes, ISSR

Kaynakça

• Altukhov, J.P. 2003. Genetic processes in populations. ICC Akademkniga, Moscow, 431 pp.
• Alves, M.J., Coelho, M.M. and Collares-Pereira, M.J. 1997. The Rutilus alburnoides complex (Cyprinidae): evidence for a hybrid origin. Journal of Zoological Systematics and Evolutionary Research, 35(1): 1–10. doi: 1111/j.1439-0469.1997.tb00398.x
• Baranyi, Ch., Gollmann, G. and Bobin, M. 1997. Genetic and morphological variability in roach Rutilus rutilus, from Austria. Hydrobiologia, 350(1-3): 13–23. doi: 1023/A:1003056311181
• Barinova, A., Yadrenkina, E., Nakajima, M. and Taniguchi, N. 2004. Identification and characterization of microsatellite DNA markers developed in ide Leuciscus idus and Siberian roach Rutilus rutilus. Molecular Ecology Notes, 4(1): 86–88.
• Bender, W., Pierre, S. and Hognes, D.S. 1983. Chromosomal walking and jumping to isolate DNA from Ace and rosy loci of bithorax complex in Drosophila melanogaster. Journal of Molecular Biology, 168: 17– doi: 1016/S00222836(83)80320-9
• Brito, R.M. and Coelho, M.M. 1999. Genetic structure of the Iberian chub, Leuciscus pyrenaicus, in the Tejo drainage. Hydrobiologia, 392(2): 169–178. doi: 1023/A:1003510909315
• Callejas, C. and Ochando, M.D. 2002. Phylogenetic relationships among Spanish Barbus species (Pisces, Cyprinidae) shown by RAPD markers. Heredity, 89(1): 36–43. doi: 10.1038/sj.hdy.6800091
• Carmona, J.A., Domínguez, J. and Doadrio, I. 2000. Congruence between allozyme and cytochrome b gene sequence data in assessing genetic differentiation within the Iberian endemic Chondrostoma lemmingii (Pisces: Cyprinidae). Heredity, 84: 721–732. doi: 1046/j.1365-2540.2000.00720.x
• Chappaz, R., Gilles, A., Miquelis, A., Cavalli, L. and Martin, J.-F. 1998. Genetic differentiation and hybridization in cyprinid Leuciscus leuciscus. Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie. 321(11): 933–940. doi: 1016/S0764-4469(99)80008-0
• Chelomina, G.N., Rozhkovan, K.V. and Ivanov, S.A. 2008. Discrimination of interspecies hybrids in natural populations of Amur sturgeon fish by means of multilocus RAPD markers. Cytology and Genetics, 5: 342-3
• Costedoat, C., Chappaz, R., Barascud, B., Guillard, O. and Gilles, A. 2006. Heterogeneous colonization pattern of European Cyprinids, as highlighted by the dace complex (Teleostei: Cyprinidae). Molecular Phylogenetics and Evolution, 41(1): 127–148. doi: 1016/j.ympev.2006.04.022
• DeMarais, B.D., Dowling, T.E., Douglas, M.E., Minckley, W.L. and Marsh, P.C. 1992. Origin of Gila seminuda (Teleostei: Cyprinidae) through introgressive hybridization: implications for evolution and conservation. Proceedings of the National Academy of Sciences of the United States of America, 89(7): 2747–2751.
• Dubut, V., Martin, J.F., Gilles, A., van Houdt, J., Chappaz, R. and Costedoat, C. 200 Isolation and characterization of polymorphic microsatellite loci for the dace complex: Leuciscus leuciscus (Teleostei: Cyprinidae). Molecular Ecology Resources, 9(4): 1179–83. doi: 10.1111/j.1755-0998.2009.02594.x.
• Durna, S., Bardakci, F. and Degerli, N. 2010. Genetic diversity of Garra rufa Heckel, 1843 (Teleostei: Cyprinidae) in Anatolia. Biochemical Systematics and Ecology, 38(1): 83–92. doi: 10.1016/j.bse.2009.12.009
• Ecology of fishes of the Ob-Irtysh basin. 2006. KMK Scientific Publications Association, Moscow, 596 pp.
• Fayazi, J., Rahimi, G., Moradi, M., Ashtyani, R. and Galledari, H. 2006. Genetic differentiation and phylogenetic relationships among Barbus xanthopterus (Cyprinidae) populations in southwest of Iran using mitochondrial DNA markers. Pakistan Journal of Biological Sciences, 9(12): 2249–2254. doi: 10.3923/pjbs.2006.2249.2254
• Häunfling, B. and Brandl, R. 2000. Phylogenetics of European cyprinids: insights from allozymes. Journal of Fish Biology, 57(2): 265–276. doi: 1111/j.1095-8649.2000.tb02171.x
• Hänfling, B., Durka, W. and Brandl, R. 2004. Impact of habitat fragmentation on genetic population structure of roach, Rutilus rutilus, in a riparian ecosystem. Conservation Genetics, 5: 247–257. doi: 10.1023/B:COGE.0000030008.20492.2c
• Imsiridou, A., Karakousis, Y. and Triantaphyllidis, C. 1997. Genetic polymorphism and differentiation among chub Leuciscus cephalus L. (Pisces, Cyprinidae) populations of Greece. Biochemical Systematics and Ecology, 25(6): 537–546. doi: 10.1016/S03051978(97)00043-4
• Kartavtsev, Y.Ph. and Hanzawa, N. 2007. Inferences in
• Leuciscinae (Pisces, Cyprinidae) phylogeny and taxonomy based on cytochrome b sequence distances and on enzyme loci diversity. Korean Journal of Genetics, 29(4): 427–435. Ketmaier, V., Cobolli, M., De Matthaeis, E. and Bianco, P.G. 1998. Allozymic variability and biogeographic relationships in two Leuciscus species complexes (Cyprinidae) from southern Europe, with the rehabilitation of the genus Telestes Bonaparte. Italian Journal of Zoology, 65(S1): 41– doi: 1080/11250009809386793
• Kizhevatov, Ya.A. and Kizhevatova A.A. 2012. The siberian dace (Leuciscus leuciscus baicalensis Dubovski, 1874) of the Sob river (lower Ob). Vestnik AGTU. A series of Fish industry, 2: 46–52.
• Koizumi, I. 2011. Integration of ecology, demography and genetics to reveal population structure and persistence: a mini review and case study of streamdwelling Dolly Varden. Ecology of Freshwater Fish, 3: 352–363. doi: 10.1111/j.1600-0633.2010.00480.x
• Korochkin, L.I., Serov, O.A., Pudovkin, A.I., Aronshtam, A.A., Borkin, L.Y., Malecki, I., Polyakova, E. and Manchenko, G.P. 1977. Genetics of isoenzymes. Nauka, Moscow, 278 pp.
• Lamphere, B.A. and Blum, M.J. 2012. Genetic estimates of population structure and dispersal in a benthic stream fish. Ecology of Freshwater Fish, 1: 75–86. doi: 1111/j.1600-0633.2011.00525.x
• Laroche, J., Durand, D.J., Bouvet, Y., Guinand, B. and Brohon, B. 1999. Genetic structure and differentiation among populations of two cyprinids, Leuciscus cephalus and Rutilus rutilus, in a large European river. Canadian Journal of Fisheries and Aquatic Sciences, 56(9): 1659–1667.
• Maurer, H. 1971. Disk-electrophoresis. Theory and practice of electrophoresis in polyacrylamide gel. Mir, Moscow, 243 pp.
• Mezhzherin, S.V. and Lisetsky, I.L. 2004. Genetic structure of crucian carp (Сypriniformes, Cyprinidae, Carassius L. 1758) populations of middle-Dnieper basin. Cytology and Genetics, 38(5): 35–44.
• Mitrofanov, I.V. 1993. The origin and taxonomy of dace genus Leuciscus Agassiz (Cyprinidae) in Central Asia and Kazakhstan. Journal of Ichthyology, 33 (6): 7637
• Mitrofanov, I.V. 2001. Phenetic relationships of Siberian and Kirghiz dace in Kazakhstan. Selevinia. Kazakhstan Journal of Zoology, 1–4: 19–25.
• Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 70: 3321– 33
• Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583–590.
• Östergren J. and Nilsson J. 2012. Importance of life-history and landscape characteristics for genetic structure and genetic diversity of brown trout (Salmo trutta L.). Ecology of Freshwater Fish, 1: 119–133. doi: 1111/j.1600-0633.2011.00529.x
• Richardson, B.J. 1986. Allozyme Electrophoresis. A Handbook for Animal Systematics and Population Studies. Academie Press, London, 410 pp.
• Rubtsova, G.A., Afanasiev, K.I., Malinina, T.V., Shitova, M.V., Rakitskaya, T.A., Prokhorovskaya, V.D. and Zhivotovsky, L.A. 2008. Differentiation of chum salmon Oncorhynchus keta Wallbaum populations as revealed with microsatellite and allozyme markers: A comparative study. Russian Journal of Genetics, 44(7): 841–848. doi: 10.1134/S0016675808070126
• Rutledge, C.J., Zimmerman, E.G. and Beitinger, Th.L. 19 Population genetic responses of two minnow species (Cyprinidae) to seasonal stream intermittency. Genetica, 80(3): 209–219. doi: 10.1007/BF00137328
• Sassaman, C., Yoshiyama, R.M. and Darling, J.D.S. 1983. Temporal stability of lactate dehydrogenase A clines of the high cockscomb Anoplarchus purpurescens. Evolution, 37(3): 472–483. doi: 10.2307/2408261
• Savin, V.A., Varnavskaya, N.V. and Shaporev, R.A. 2009. Patterns of genetic diversity in population complexes of pacific chum salmon Oncorhynchus keta
• Walbaum, from Asia and America, inferred from allozyme polymorphism data. Russian Journal of Genetics, 45(6): 726–737. doi: 10.1134/S0016675809060125
• Shivraman, G.K., Barat, A., Ali, S., Pandey, N.N., Joshi, K.D. and Mahanta, P.C. 2010. An analysis of genetic diversity among indian coldwater fishes (Pisces: Cyprinidae) using RAPD markers. The IUP Journal of Genetics and Evolution, 3(2), 31–40.
• Teletchea, F. 2009. Molecular identification methods of fish species: reassessment and possible applications. Reviews in Fish Biology and Fisheries, 3: 265-293. doi: 1007/s11160-009-9107-4.
• Wilson, A.C., Maxson, L.R. and Sarich V.M. 1974. Two types of molecular evolution: evidence from studies of interspecific hybridization. Proceedings of the National Academy of Sciences of the United States of America, 71: 2843–2847.
• Vasil'eva, E.D., Mina, M.V. and Pavlinov, I.J. 1993. Analysis of the phenetic diversity in dace (subgenus Leuciscus, Cyprinidae). Position of Leuciscus danilewskii. Journal of Ichthyology, 33 (4): 475-485.
• Vrijenhoek, R.C. 1998. Conservation genetics of freshwater fish. Journal of Fish Biology, 53: 394–412.
• Yadrenkina, E.N., Interesova, E.A., Yadrenkin, A.V. and Khakimov, R.M. 2005. On the spatial differentiation of populations of cyprinid fish Chany Lake (Western Siberia). Siberian Journal of Ecology, 12(2): 293–304. Yeh, F.C., Yang, R. and Boyle, T. 1999. POPGENE. Version 1.31. / University of Alberta and Centre for International Forestry Research.
• Zhigileva, O.N., Ozhirel’ev, V.V., Brol, I.S. and Pozhidaev, V.V. 2010. Population structure of three fish species (Cypriniformes: Cyprinidae) living in rivers of the Ob-Irtysh basin, by the data of isoenzyme analysis. Journal of Ichthyology, 50(9): 778–787. doi: 1134/S0032945210090110