Comparative Efficacy of Three Anesthetic Agents on Juvenile African Catfish, Clarias gariepinus (Burchell, 1822)

Yıl 2013, Cilt: 13 Sayı: 1, - , 01.02.2013

Fatih Öğretmen Kaya Gökçek

https://doi.org/10.4194/1303-2712-v13_1_07

Öz

In this study, the efficacy of three anaesthetic agents (clove oil, 2-phenoxyethanol and eugenol) was compared in captive-bred African catfish, Clarias gariepinus (Burchell, 1822). The lowest effective concentrations based on the efficacy criteria of complete anesthesia induction within 180 s and recovery within 300 s were determined to be 50 mg L (induction 193±62 s and recovery time 251±32 s) for clove oil, 750 µl L (induction 145±24 s and recovery time 174±10 s) for 2phenooxyethanol, and 50 mg L (induction 197±29 s and recovery time 310±17 s) for eugenol. The onset of individual phases of anesthesia and recovery times depended significantly on the concentration of the anaesthetic used (P<0.05). An inverse exponential relationship was observed between concentrations of anaesthetic and induction time, whereas exponential relationships were observed between concentrations and recovery times for all anaesthetic agents evaluated. The final conclusion of this study, clove oil is the most suitable agent for juvenile African catfish from the three anesthetics tested.

Anahtar Kelimeler

Clove oil, 2-phenoxyethanol, eugenol, induction time, recovery time.

Comparative Efficacy of Three Anesthetic Agents on Juvenile African Catfish, Clarias gariepinus (Burchell, 1822)

Öz

Bu çalışmada, üç anestezik maddenin (karanfil yağı, fenoksiethanol ve eugenol) etkinliği kültür şartları altında yetiştirilmiş Karabalık’larda karşılaştırılmıştır. Etkin en düşük doz kriteri olan 180 s içinde bayılma ve 300 s içinde ayılma hali karanfil yağında 50 mg L (bayılma 193±62 s ve ayılma 251±32 s), fenoksiethanolde 750 µl L (bayılma 145±24 s ve ayılma 174±10 s) ve eugenolde 50 mg L (bayılma 197±29 s ve ayılma 310±17 s) dozlarında elde edilmiştir. Bireysel olarak bayılma ve ayılma hallerinin başlangıcı, kullanılan anestezik maddenin dozuna bağlı olarak istatistiki açıdan farklı bulunmuştur (P<0,05). Anestezik madde konsantrasyonu ile bayılma süresi arasında ters bir üssel ilişki tespit edilmiş, ancak anestezik madde konsantrasyonu ile ayılma süreleri arasındaki ilişki üssel olarak ölçülmüştür. Bu çalışmanın sonucunda, kullanılan üç anestezik maddeden Karabalıklar için en uygun olanın karanfil yağı olduğu söylenebilir.

Anahtar Kelimeler

Karanfil yağı, fenoksiethanol, eugenol, bayılma süresi, ayılma süresi.

Kaynakça

• Burka, J.F., Hammell, K.L., Horsberg, T.F., Johnson, G.R., Rainnie, D.J. and Speare, D.J. 1997. Drugs in salmonid aquaculture. J. Vet. Pharmacol. Ther., 20: 333-3
• Coyle, S.D., Durborow, R.M. and Tidwell, H.J. 2004. Anaesthetic in Aquaculture: Southern Regional Aquaculture Center (SRAC) Publication, 3900. Texas, 6 pp.
• Endo, T., Ogishima, K., Tanaka, H. and Ohshima, S. 1972. Studies on the anaesthetic eject of eugenol in some freshwater fishes. Bulletin of the Japanese Society of Scientific Fisheries, 38: 761-767.
• Gilderhus, P.A. and Marking, L.L. 1987. Comparative efficacy of 16 anaesthetic chemicals on rainbow trout. N. Am. J. Fish Manag., 7: 288-292.
• Gomes, L.C., Chiparri-Gomes, A.R., Lopes, N.P., Roubach, R. and Araujo-Lima, C.A.R.M. 2001. Efficacy of benzocaine as an anesthetic in juvenile tambaqui Colossoma macropomum. J. World Aqua. Sco., 32: 426-4
• Gullian, M. and Villanueva. J. 2009. Efficacy of tricaine methanesulphonate and clove oil as anaesthetics for juvenile cobia Rachycentron canadum. Aquaculture Res., 40: 852-860.
• Heo, G.J. and Shin, G. 2010. Efficacy of benzocaine as an anaesthetic for Crucian carp (Carassius carassius). Vet. Anaesth. Analg., 37: 132-135.
• Hikasa, Y., Takase, K., Ogasawara, T. and Ogasawara, S. 19 Anaesthesia and recovery with tricaine methanesulfonate, eugenol and tripental sodium in the carp, Cyprinus carpio. Japanese Journal of Veterinary Science, 48: 341-351. Hoskonen, P. and Pirhonen, J. 2004. Temperature ejects on anaesthesia with clove oil in six temperature-zone fishes. Journal of Fish Biology, 64: 1136-1142.
• Hseu, J.R., Yeh, S.L., Chu, Y.T. and Ting, Y.Y. 1998. Comparison of efficacy of five anaesthetic goldlined sea bream, Sparus sarba. Acta. Zool., Taiwan, 9: 35
• Iversen, M., Finstad, B., Mac Kinley, R.S. and Eliiassen, R.S. 2003. The efficacy of metomidate, clove oil, Aqui-S and Benzoak as anaesthetics in Atlantic salmon (Salmo salar L.) smolts and their potential stress-reducing capacity. Aquaculture, 221: 549-566. Keene, J.L.I., Noakes, D.L.G., Moocia, R.D. and Soto, C.G. 19 The efficacy of clove oil as an anesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research, 29: 89-101.
• Marking, L.L. and Meyer, E.P. 1985. Are better anesthetics needed in fisheries? Fisheries, 10: 2-5.
• Mattson, N.S. Riple, T.H. 1989. Metomidate, a better anaesthetic for cod (Gadus morhua) in comparison with benzocaine, MS-222, chlorobutanol and phenoxyethanol. Aquauculture, 83: 89-94.
• Mc Farland, W.N. 1960. The use of anaesthetics for the handling and the transport of fishes. California Fish Game, 46: 407-431.
• Munday, P.L. and Wilson, S.K. 1997. Comparative efficacy of clove oil and other chemicals in anesthetization of Pomacentrus amboinensis, a coral reef fish. Journal of Fish Biology, 51:931-938.
• Mylonas, C.C., Cardinaletti, G., Sigelaki, I. and PolzonettiMagni, A. 2005. Comparative efficacy of clove oil and 2-phenoxyethanol as anaesthetics in aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus auratus) at different temperatures. Aquaculture, 246: 467-481.
• Pawar, H.B., Sanaye, S.V., Sreepada, R.A., Harish, V., Suryavanshi, U. and Ansari, Z.A. 2011. Comparative efficacy of four anaesthetic agents in the yellow seahorse, Hippocampus kuda (Bleeker, 1852). Aquaculture, 311: 155-161.
• Ross, L.G. and Ross, B. 1999. Anesthetics and sedative techniques fro aquatic animals. Blackwell Sci., Oxford, 159 pp.
• Theinpoint, D. and Niemegeers, C.J.E. 1965. 7464- a new potenet anesthetics in fish. International Zoo. Year Book, 5: 202-205.
• Treves-Brown, K.M. 2000. Anaesthetics in applied fish pharmacology. Kluwer Academic Publishers, Dordrecht, Netherlands: 206-217.
• Tytler, P. and Hawkins, A.D. 1981. Vivisection, anesthetics and minor surgery. In: A.D. Hawkins (Ed.) Aquarium Sys. Academic Press, New York: 247-278.
• Velisek, J., Wlasow, T., Gomulka, P., Svobodava, Z., Novotny, L. and Ziomek, A. 2006. Effects of clove oil anaesthesia on catfish (Siluris glanis L.). Acta. Vet. Brno., 75: 99-106.
• Velisek, J., Stara, A., Li, Z.H., Silovska, S. and Turek, J. 20 Comparison of effects of four anaesthetics on blood chemical profiles and oxidative stress biomarkers in rainbow trout. Aquaculture, 310: 3693
• Woody, C.A., Nelson, J. and Ramstad, K. 2002. Clove oil as an anaesthetic for adult sockeye salmon: field trails. Journal of Fish Biology, 60: 3450-347.
• Weber, R.A., Peleterio, J.B., Garcia Martin, L.O. and Aldegunde, M. 200 The efficacy of 2phenoxyethanol, metomidate, clove oil and MS-222 as anaesthetic agents in the Senegalese sole (Solea senegalensis Kaup, 1858). Aquaculture, 288: 147-150. Zar, J.H. 1999. Bio-statistical Analysis, 4 th Edition. Prentice Hall, New Jersey, 929 pp.