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Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae

Yıl 2010, Cilt: 10 Sayı: 1, - , 01.02.2010

Öz

The ontogenesis and formation stages of exocrine pancreas in European sea bass (D. labrax) larvae were investigated from hatching to 40 days after hatching (DAH). Histological and enzymatical techniques were used to explain the functional development of the pancreas in D. labrax with the expression of trypsinogen activity. The incipient pancreas appeared as a lamination of the dorsal wall of the digestive tract. It was observed that the primary visible indication of exocrine cell differentiation was polarization. The first zymogen granules and pancreas with exocrine polyhedral cells appeared on 6 DAH and became abundant as a compact structure located dorsal and slightly posterior to the liver. At the same time, firstly, anus and then mouth were opened, and total lengths of larvae were determined as 3.47±0.26 mm. Until larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery enclosure, the stomach, the upper intestine and the pyloric caeca. On the other hand, zymogen granules were more numerous and larger, and a greater quantity of material was carried by the ducts, indicating an increased cellular activity. The specific activity of trypsin was determined as early as after hatching (42.54±6.8 mU/mg protein-1) at 4.28±0.2 mm total length of larvae and increased immediately during the following days especially after exogenous feeding. The highest tryptic activity was detected on 30 DAH as 122.45±11.76 mU/mg protein-1. It is concluded that exocrine pancreas organogenesis is the main critical step of the zymogen granules and trypsin activity is present as early as after hatching and continuously increasing with larval period of D. labrax.

Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae

Yıl 2010, Cilt: 10 Sayı: 1, - , 01.02.2010

Öz

The ontogenesis and formation stages of exocrine pancreas in European sea bass (D. labrax) larvae were investigated from hatching to 40 days after hatching (DAH). Histological and enzymatical techniques were used to explain the functional development of the pancreas in D. labrax with the expression of trypsinogen activity. The incipient pancreas appeared as a lamination of the dorsal wall of the digestive tract. It was observed that the primary visible indication of exocrine cell differentiation was polarization. The first zymogen granules and pancreas with exocrine polyhedral cells appeared on 6 DAH and became abundant as a compact structure located dorsal and slightly posterior to the liver. At the same time, firstly, anus and then mouth were opened, and total lengths of larvae were determined as 3.47±0.26 mm. Until larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery enclosure, the stomach, the upper intestine and the pyloric caeca. On the other hand, zymogen granules were more numerous and larger, and a greater quantity of material was carried by the ducts, indicating an increased cellular activity. The specific activity of trypsin was determined as early as after hatching (42.54±6.8 mU/mg protein-1) at 4.28±0.2 mm total length of larvae and increased immediately during the following days especially after exogenous feeding. The highest tryptic activity was detected on 30 DAH as 122.45±11.76 mU/mg protein-1. It is concluded that exocrine pancreas organogenesis is the main critical step of the zymogen granules and trypsin activity is present as early as after hatching and continuously increasing with larval period of D. labrax.

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Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

H. Okan Kamaci Bu kişi benim

Deniz Çoban Bu kişi benim

Cüneyt Suzer Bu kişi benim

Bahadır Aksu Bu kişi benim

Şahin Saka Bu kişi benim

Kürşat Firat Bu kişi benim

Yayımlanma Tarihi 1 Şubat 2010
Yayımlandığı Sayı Yıl 2010 Cilt: 10 Sayı: 1

Kaynak Göster

APA Kamaci, H. O., Çoban, D., Suzer, C., Aksu, B., vd. (2010). Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae. Turkish Journal of Fisheries and Aquatic Sciences, 10(1).
AMA Kamaci HO, Çoban D, Suzer C, Aksu B, Saka Ş, Firat K. Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae. Turkish Journal of Fisheries and Aquatic Sciences. Şubat 2010;10(1).
Chicago Kamaci, H. Okan, Deniz Çoban, Cüneyt Suzer, Bahadır Aksu, Şahin Saka, ve Kürşat Firat. “Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus Labrax) Larvae”. Turkish Journal of Fisheries and Aquatic Sciences 10, sy. 1 (Şubat 2010).
EndNote Kamaci HO, Çoban D, Suzer C, Aksu B, Saka Ş, Firat K (01 Şubat 2010) Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae. Turkish Journal of Fisheries and Aquatic Sciences 10 1
IEEE H. O. Kamaci, D. Çoban, C. Suzer, B. Aksu, Ş. Saka, ve K. Firat, “Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae”, Turkish Journal of Fisheries and Aquatic Sciences, c. 10, sy. 1, 2010.
ISNAD Kamaci, H. Okan vd. “Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus Labrax) Larvae”. Turkish Journal of Fisheries and Aquatic Sciences 10/1 (Şubat 2010).
JAMA Kamaci HO, Çoban D, Suzer C, Aksu B, Saka Ş, Firat K. Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae. Turkish Journal of Fisheries and Aquatic Sciences. 2010;10.
MLA Kamaci, H. Okan vd. “Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus Labrax) Larvae”. Turkish Journal of Fisheries and Aquatic Sciences, c. 10, sy. 1, 2010.
Vancouver Kamaci HO, Çoban D, Suzer C, Aksu B, Saka Ş, Firat K. Exocrine Pancreas Development and Trypsin Expression in Cultured European Sea Bass (Dicentrarchus labrax) Larvae. Turkish Journal of Fisheries and Aquatic Sciences. 2010;10(1).